Quanzhou, China
Quanzhou, China

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Yang B.,Chinese PLA General Hospital | Yu R.-l.,Chinese PLA General Hospital | Tuo S.,Hospital of Chinese PLA | Tuo C.-w.,Hospital of Chinese PLA | And 6 more authors.
PLoS ONE | Year: 2012

Background: Human xenograft models, resulting from orthotopic transplantation (implantation into the anatomically correct site) of histologically intact tissue into animals, are important for investigating local tumor growth, vascular and lymphatic invasion at the primary tumor site and metastasis. Methodology/Principal Findings: We used surgical orthotopic transplantation to establish a nude mouse model of primary hepatic lymphoma (PHL), HLBL-0102. We performed orthotopic transfer of the HLBL-0102 tumor for 42 generations and characterized the tumor cells. The maintenance of PHL characteristics were supported by immunohistochemical and cytogenetic analysis. We also report the antitumor effect of Cantide, an antisense phosphorothioate oligonucleotide against hTERT, on the growth of HLBL-0102 tumors. We showed a significant, dose-dependent inhibition of tumor weight and serum LDH activity in the orthotopically transplanted animals by Cantide. Importantly, survival was prolonged in Cantide-treated HLBL-0102 tumor-bearing mice when compared to mock-treated mice. Conclusions/Significance: Our study provided the basis for the development of a clinical trial protocol to treat PHL. © 2012 Yang et al.

Wang M.-H.,Hospital of Chinese PLA | Xu J.-F.,Hospital of Chinese PLA
International Eye Science | Year: 2012

AIM: To observe the changes of preoperative and postoperative tear film in the surgical treatment for pterygium by pterygium excision combined with autologous limbus epithelial transplantation, and analyse the influence of pterygium on the function of tear film. METHODS: A total of 24 cases (24 eyes) of pterygium underwent pterygium excision combined with autologous limbus epithelial transplantation. All patients were measured by Schirmer test and tear film break-up test (BUT) on 1 day before operation, 1 month and 2 months after operation respectively. RESULTS: The result of Schirmer test were 15.5±7.81 mm, 15.76±7.39 mm and 16.00±8.05 mm, and BUT were 8.15±3.611, 10.05±3.03, and 10.50±4.26 seconds on 1 day before operation, 1 month and 2 months after operation respectively. The difference of BUT had statistical significance between preoperation and postoperation (P<0.05), but there was no significant change in Schirmer test in different time (P >0.05). CONCLUSION: The form of the ocular surface and the stability of tear film were restored by pterygium excision combined with autologous limbus epithelial transplantation.

Yu Y.-z.,The 474th Hospitol of Chinese PLA | Mi C.-b.,Xinjiang Medical University | Zhao Y.-p.,Hospital of Chinese PLA | Zu Q.,Xinjiang Medical University | And 3 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Various studies have shown the association between masticatory muscle morphology and craniofacial morphology. However, the interaction between masticatory muscle morphology and dental arch form remai ns poorly understood. OBJECTIVE: To investigate the influence of masticatory muscle morphology and function on dental arch forms and the underlying mechanisms. METHODS: A total of 69 males with normal occlusion, aged 23.6 years (mean 18-28 years) were included in this study. Ultrasound scanning was used to measure the perimeter, area, width, length, mean thickness and maximal thickness of masseter muscle and the mean thickness and maximal thickness of anterior temporal muscle. All items were measured under mandibular postural position. The arch forms were measured. Correlation analysis was done between the ultrasound data and dental arch form. RESULTS AND CONCLUSION: There was a positive correlation between the cross-section area of masseter muscle and the length of maxillary antero-middle dental arch, between the maximal thickness of masseter muscle and the width of mandibular middle dental arch and the perimeter of maxillary dental arch (P < 0.05). There was a negative correlation between the thickness of anterior temporal muscle and the length of maxillary anterior and antero-middle dental arch (P < 0.05). The results indicate that the function and morphology of masseter and temporal muscle may be one of the factors influencing the morphology of dental arch.

Zhao Y.,Hospital of Chinese PLA
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2011

BACKGROUND: Pelvic fractures are more common in clinic. There are various internal fixation methods for implantation that are difficult. If not treated properly, it can cause pelvic soft tissue injury, skin necrosis, infection, leading to clinical failure of internal fixation as one important reason. OBJECTIVE: To investigate the optimal internal fixation therapy for pelvic fractures and to evaluate its effect. METHODS: A computer-based online search of VIP database (2004-01/2011) was conducted to retrieve articles regarding screw and plate internal fixation of treatment of pelvic fractures using the keywords of "pelvic fracture, internal fixation, effect evaluation". RESULTS AND CONCLUSION: Pelvic fracture is a high-energy injury with high morbidity and high mortality, and the incidence is increased year by year in clinic. Relevant biomechanical studies are useful to explain the action mechanism and fracture type of pelvic fractures and play a very important role in the final choice of treatment. Anatomic reduction and solid fixation of pelvic fractures as well as screws and plate internal fixation are very important. To develop an implant with small wound, low infection rate, stable fixation and good compatibility is the current direction in the treatment of unstable pelvic fractures.

PubMed | Hospital of Chinese PLA
Type: Journal Article | Journal: Acta oto-laryngologica | Year: 2010

The three most common GJB2 mutations found in the Chinese populations, c.235delC, c.299-300delAT, and c.176-191de1 (16) bp, cannot form gap junctons (GJs) in the plasma membrane. These mutant proteins were retained in the endoplasmic reticulum (ER), suggesting that ER stress (ERS) and subsequent ERS-induced cell death may be responsible for hearing loss caused by these GJB2 truncation mutations.The objective of this study was to investigate the subcellular location of the protein products of three GJB2 mutants (c.235de1C, c.299-300delAT, and c.176-191de1 (16) bp) and to explore the deafness mechanism caused by these GJB2 truncation mutations.Mutant-eGFP fusion protein vectors were constructed by PCR and TA cloning. HEK293 cells were transfected by a liposome-mediated method. Transfected cells were incubated with ER-Tracker and observed under a confocal microscope.Cells transfected with wild type gave characteristic punctuate patterns of GJs in the cell membrane. In contrast, c.235de1C, c.299-300delAT, and c.176-191de1 (16) bp mutant proteins were found to be trapped in the ER, and were therefore unable to form GJs in the plasma membrane.

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