Peng W.,Fudan University |
Hu J.,Hospital No. 455 of the PLA |
Zhu X.-D.,Fudan University |
Liu X.,Fudan University |
And 3 more authors.
Tumor Biology | Year: 2014
Vemurafenib is a selective and potent small molecule inhibitor of the V600 mutant form of the BRAF protein used in the treatment of melanoma and colorectal cancer. However, vemurafenib has less effect in BRAF mutant colorectal cancer due to the resistance of tumor cell to vemurafenib. To verify whether or not miR-145, a short RNA molecule of microRNA which has been supposed to be a tumor suppressor, is involved in this process, we established vemurafenib-resistant cell line colo205/V and found that the miR-145 expression was significantly downregulated in colo205/V cells compared to normal colo205 cells. Moreover, the overexpression of miR-145 could increase the sensitivity of colo205/V cells to vemurafenib both in vitro and in vivo. In conclusion, miR-145 might be used as a therapeutic target in the treatment of colorectal cancer patients with BRAF V600E mutation. © 2013 International Society of Oncology and BioMarkers (ISOBM).
Gu X.,Hospital No. 455 of the PLA |
Miao X.,Hospital No. 455 of the PLA |
Xu J.,Hospital No. 455 of the PLA |
Cao J.,Hospital No. 455 of the PLA
BioScience Trends | Year: 2012
The present study investigated the effect of the VEGF165 gene on adhesion, migration, and proliferation of endothelial progenitor cells (EPCs) derived from peripheral blood of rabbits. Peripheral blood mononuclear cells were isolated from rabbits by density gradient centrifugation with Ficoll-Plaque Plus. EPCs were characterized by immunofluorescence and immunostaining. A pcDNA 3.0-hVEGF165 expression vector was constructed and EPCs were transfected with the pcDNA 3.0-hVEGF165 gene. The EPCs derived from peripheral blood of rabbits were successfully transfected with pcDNA 3.0-hVEGF165. ELISA showed that the expression of VEGF165 increased significantly in the EPCs transfected with the hVEGF165gene compared to control cells. Compared to control EPCs, EPCs transfected with the hVEGF165 gene hadsignificantly enhanced adhesion, migration, and proliferative ability in vitro.