Shanghai, China
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Chen M.,Zhejiang University | Duan H.,Fudan University | Xu L.,Zhejiang University | Chen T.,Hospital No 455 Of The Pla
Pakistan Journal of Medical Sciences | Year: 2012

Cavernous sinus metastasis of hypopharyngeal carcinoma is rare. We present a 57-year old Chinese man who was diagnosed as a case with hypopharyngeal carcinoma (T3N2bM0). He received the radical surgery and radiotherapy. After six months, it was found that the metastasis of hypopharyngeal carcinoma appeared in the right cavernous sinus. The patient was treated with Gamma knife radiosurgery. However, 10 months later, the clinical sign and mass reappeared, then Gamma knife radiosurgery was used again at the double dose. After 17 months, the tumor had extended into the right temporal bone and showed metastasis in the abdomen and lung without the metastasis in the neck or the recurrence in the hypopharynx. The patient survived 29 months. Gamma knife radiosurgery may be effective in the treatment of patients with cavernous sinus metastasis.

Peng W.,Fudan University | Hu J.,Hospital No 455 Of The Pla | Zhu X.-D.,Fudan University | Liu X.,Fudan University | And 3 more authors.
Tumor Biology | Year: 2014

Vemurafenib is a selective and potent small molecule inhibitor of the V600 mutant form of the BRAF protein used in the treatment of melanoma and colorectal cancer. However, vemurafenib has less effect in BRAF mutant colorectal cancer due to the resistance of tumor cell to vemurafenib. To verify whether or not miR-145, a short RNA molecule of microRNA which has been supposed to be a tumor suppressor, is involved in this process, we established vemurafenib-resistant cell line colo205/V and found that the miR-145 expression was significantly downregulated in colo205/V cells compared to normal colo205 cells. Moreover, the overexpression of miR-145 could increase the sensitivity of colo205/V cells to vemurafenib both in vitro and in vivo. In conclusion, miR-145 might be used as a therapeutic target in the treatment of colorectal cancer patients with BRAF V600E mutation. © 2013 International Society of Oncology and BioMarkers (ISOBM).

Gu X.,Hospital No 455 Of The Pla | Miao X.,Hospital No 455 Of The Pla | Xu J.,Hospital No 455 Of The Pla | Cao J.,Hospital No 455 Of The Pla
BioScience Trends | Year: 2012

The present study investigated the effect of the VEGF165 gene on adhesion, migration, and proliferation of endothelial progenitor cells (EPCs) derived from peripheral blood of rabbits. Peripheral blood mononuclear cells were isolated from rabbits by density gradient centrifugation with Ficoll-Plaque Plus. EPCs were characterized by immunofluorescence and immunostaining. A pcDNA 3.0-hVEGF165 expression vector was constructed and EPCs were transfected with the pcDNA 3.0-hVEGF165 gene. The EPCs derived from peripheral blood of rabbits were successfully transfected with pcDNA 3.0-hVEGF165. ELISA showed that the expression of VEGF165 increased significantly in the EPCs transfected with the hVEGF165gene compared to control cells. Compared to control EPCs, EPCs transfected with the hVEGF165 gene hadsignificantly enhanced adhesion, migration, and proliferative ability in vitro.

This study sought to evaluate the effects of selective brain cooling on the expression of intercellular adhesion molecule-1 (ICAM-1) mRNA and infiltration of polymorphonuclear leukocytes (PMNLs) and monocytes/macrophages (MPhi) during global cerebral ischemia/ reperfusion (I/R). Global ischemia of the brain was produced by four-vessel occlusion for 30 min followed by reperfusion for 240 min. Thirty-five SD rats were randomly divided into five groups: group I had no ischemia and reperfusion; groups II, III, IV, and V were subjected to ischemia for 30 min at 37 degrees C and reperfusion for 240 min at 37, 35, 32, and 28 degrees C, respectively. Cerebral tissue samples were taken for pathological examination of the infiltration of PMNLs and MPhi and to detect ICAM-1 mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR). The expression of ICAM-1 mRNA and infiltration of PMNLs and MPhi increased more markedly in group II than in group I (p < 0.01), suggesting that hypothermia evidently inhibited ICAM-1 mRNA expression and PMNL and MPhi infiltration in the damaged cerebral tissue. In addition, significant differences were also found between group III and group II (p < 0.05) and among groups IV, V, and II (p < 0.01). These results suggest that I/R injury induces ICAM-1 mRNA expression and PMNL and MPhi infiltration in SD rats and that selective brain cooling, and especially moderate hypothermia (28-32 degrees C), may provide better cerebral protection by markedly inhibiting the expression of ICAM-1 mRNA while decreasing the infiltration of PMNLs and MPhi in the brain.

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