Hospital General Doctor Manuel Gea Gonzalez

Tlalpan, Mexico

Hospital General Doctor Manuel Gea Gonzalez

Tlalpan, Mexico

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Garcia G.,National Autonomous University of Mexico | Ramos F.,National Autonomous University of Mexico | Perez R.G.,National Autonomous University of Mexico | Yanez J.,National Autonomous University of Mexico | And 4 more authors.
Journal of Medical Microbiology | Year: 2014

Veterinary medicine has focused recently on reptiles, due to the existence of captive collections in zoos and an increase in the acquisition of reptiles as pets. The protozoan parasite, Entamoeba can cause amoebiasis in various animal species and humans. Although amoebiasis disease is remarkably rare in most species of chelonians and crocodiles, these species may serve as Entamoeba species carriers that transmit parasites to susceptible reptile species, such as snakes and lizards, which can become sick and die. In this study, we identified the Entamoeba species in a population of healthy (disease-free) chelonians, and evaluated their diversity through the amplification and sequencing of a small subunit rDNA region. Using this procedure, three Entamoeba species were identified: Entamoeba invadens in 4.76% of chelonians, Entamoeba moshkovskii in 3.96% and Entamoeba terrapinae in 50%. We did not detect mixed Entamoeba infections. Comparative analysis of the amplified region allowed us to determine the intra-species variations. The E. invadens and E. moshkovskii strains isolated in this study did not exhibit marked differences with respect to the sequences reported in GenBank. The analysis of the E. terrapinae isolates revealed three different subgroups (A, B and C). Although subgroups A and C were very similar, subgroup B showed a relatively marked difference with respect to subgroups A and C (Fst50.984 and Fst51.000, respectively; 10-14% nucleotide variation, as determined by BLAST) and with respect to the sequences reported in GenBank. These results suggested that E. terrapinae subgroup B may be either in a process of speciation or belong to a different lineage. However, additional research is necessary to support this statement conclusively. © 2014 SGM.


Villalobos G.,Institute Investigaciones Biomedicas | Martinez-Hernandez F.,Hospital General Doctor Manuel Gea Gonzalez | De La Torre P.,Institute Investigaciones Biomedicas | Laclette J.P.,Institute Investigaciones Biomedicas | Espinoza B.,Institute Investigaciones Biomedicas
American Journal of Tropical Medicine and Hygiene | Year: 2011

The purpose of this study was to conduct an entomological analysis, determination of feeding sources, and molecular identification of triatomines in five communities of the Istmo de Tehuantepec, Oaxaca. The only found species in two of five searched communities (San Mateo del Mar and Tehuantepec City) was Triatoma phyllosoma. Colonization indices were high in both communities. In San Mateo del Mar, the insects were found indoors and in Tehuantepec City in peridomestic areas. The Trypanosoma cruzi infection indices were 2.1% in San Mateo del Mar and 39.4% in Tehuantepec City. This difference could be related to the high numbers of triatomine feeding on hens in the former community. In contrast, in Tehuantepec, dogs were the principal triatomine feeding sources. All nymphs and adults that were genetically analyzed belonged to the species T. phyllosoma. Low levels of genetic variation were found between vectors from both communities. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.


Uribe-Koch L.M.,Servicio de Ginecologia y Obstetricia | Estrada-Hernandez M.R.,Medico adscrito al servicio de Anatomia patologica | Ojendiz-Nava R.C.,Hospital General Doctor Manuel Gea Gonzalez
Ginecologia y Obstetricia de Mexico | Year: 2013

The pyometra is a rare condition, with an incidence of less than 1%. In patients with cervical cancer, spontaneous rupture of pyometra manifests as a generalized peritonitis, which is extremely rare, in the literature only seven cases are described. This paper reports the case of a patient with a history of postmenopausal vaginal bleeding one month before her admission to the hospital; she attended because of acute abdomen. The CT scan reported air in the abdominal cavity and the uterus with air at the periphery, so she underwent an exploratory laparotomy in which purulent material was found with two perforations in the uterine fundus. She underwent total abdominal extrafacial hysterectomy with histopathological diagnosis of keratinizing squamous cell carcinoma, moderately differentiated.


Villalobos G.,National Polytechnic Institute of Mexico | Martinez-Ibarra J.A.,University of Guadalajara | Martinez-Hernandez F.,Hospital General Doctor Manuel Gea Gonzalez | Lopez-Alcaide S.,National Autonomous University of Mexico | Alejandre-Aguilar R.,National Polytechnic Institute of Mexico
Journal of Vector Ecology | Year: 2012

The control of triatomine insects is necessary because these insects are the principal vectors of Trypanosoma cruzi, the agent of Chagas disease. Nevertheless, some of these vectors, such as Triatoma protracta, have not been studied adequately and their importance and taxonomic status has not yet been defined in detail and must be reevaluated in view of the continuing taxonomic uncertainties associated with the species. To help clarify the taxonomic status of T. protracta, the eggs and genital plates of two morphotypes were analyzed. Qualitative and quantitative morphological differences were observed in two morphotypes, designated T. p. protracta and T. p. nahuatlae according to Ryckman (1962). The morphotype T. p. protracta exhibited large and wide eggs with pores forming large padded polygonal structures, whereas the eggs of the morphotype T. p. nahuatlae were small and smooth. The size of the 9th genital urosternite was longer and wider in females in contrast to males in both morphotypes. However, these size differences were relatively greater in T. p. protracta. The high morphological variation found between the morphotypes of T. protracta suggests that they should be separated. Accordingly, it is probable that this group should be re-classified. © 2012 The Society for Vector Ecology.


Ibarra-Arce A.,Hospital General Doctor Manuel Gea Gonzalez | Garcia-Alvarez M.,Hospital General Doctor Manuel Gea Gonzalez | Cortes-Gonzalez D.,Hospital General Doctor Manuel Gea Gonzalez | Ortiz de Zarate-Alarcon G.,Hospital General Doctor Manuel Gea Gonzalez | And 5 more authors.
Meta Gene | Year: 2015

Cleft lip with or without cleft palate (CL/P) is one of the most common birth defects; it is a multifactorial disease affecting. >. 1/1,000 live births in Europe, and its etiology is largely unknown, although it is very likely genetic and environmental factors contribute to this malformation. Orofacial development is a complex process involving many genes and signaling pathways. Mutations in the gene for the interferon regulatory factor 6 (. IRF6) cause a hereditary dominant malformation syndrome including CL/P, and polymorphisms are associated with non-syndromic CL/P (MIM 119530). Five SNPs at the locus with high heterozygosity in Caucasian populations were chosen for the present research due to their very strong association with CL/P. A case-parent trio study was performed using 292 samples from Mexico. Association with the rs1319435-C/C genotype (. P=. 0.02) was found in patients (73) as compared to pseudocontrols (219), while the genotype rs1319435-T/C was related with protection (. P=. 0.041) in the triad design. Significant over-transmission of the G allele for marker rs2235375 (. P=. 0.049) was found. Only the TACGT haplotype was diminished in the affected child, either in single (. P=. 0.0208) or double (. P=. 0.0208) dose. The pairwise analysis showed rs2235543 and rs2235371 were in strong linkage disequilibrium. These results point to a substantial contribution of IRF6 in the etiology of non-syndromic CL/P in a sample of the Mexican population. © 2015 The Authors. Published by Elsevier B.V.


Sierra-Murguia M.,National Autonomous University of Mexico | Vite-Sierra A.,National Autonomous University of Mexico | Ramos-Barragan V.,Hospital General Doctor Manuel Gea Gonzalez | Lopez-Hernandez J.C.,Hospital General Doctor Manuel Gea Gonzalez | And 2 more authors.
International Journal of Psychology and Psychological Therapy | Year: 2012

Bariatric surgery has proven to be the most effective treatment for severe obesity. Weight loss and long-term maintenance depend on patient's ability to implement permanent lifestyle changes. Presurgical psychological evaluation and intervention are proposed for a better post surgical prognosis. The aim of present study was to make a psychosocial profile with the results of psychological evaluation performed to 129 bariatric surgery candidates and to determine associations between psychological variables with obesity level. Patients were evaluated with an oral interview, the MINI International Neuropsychiatrie Interview, and the following self-reported instruments: Beck Depression Inventory, Anxiety Sensitivity Inventory, Stress Control Perception, Risk Factors related to Eating Behavior Disorders Scale, Quality of Life and Health Inventory. Study population was divided in groups according to obesity level, evaluation results were compared according to these groups. Results in present study show a high prevalence of psychiatric disorders; obesity level was not directly associated with level of psychopathology. Another finding is that at higher level of obesity, there is less concern about weight and food and also there is a significant higher perception of family support. The group of patients with higher concerns about weight and food had lower BMI and less perception of family support. Copyright © 2012 AAC.


PubMed | Hospital General Doctor Manuel Gea Gonzalez, National Autonomous University of Mexico, National Polytechnic Institute of Mexico, Hospital Doctor Manuel Gea Gonzalez and Milpa Alta Institute of Technology
Type: Journal Article | Journal: PloS one | Year: 2016

RhoGDI proteins have been implicated in several human cancers; changes in their expression levels have shown pro- or anti-tumorigenic effects. Pancreatic Ductal Adenocarcinoma (PDAC) is a complex pathology, with poor prognosis, and most patients die shortly after diagnosis. Efforts have been focused on understanding the role of RhoGDIs in PDAC, specially, RhoGDI1 and RhoGDI2. However, the role of RhoGDI3 has not been studied in relation to cancer or to PDAC. Here, we characterized the expression and functionality of RhoGDI3 and its target GTPases, RhoG and RhoB in pancreatic cell lines from both normal pancreatic tissue and tissue in late stages of PDAC, and compared them to human biopsies. Through immunofluorescences, pulldown assays and subcellular fractionation, we found a reduction in RhoGDI3 expression in the late stages of PDAC, and this reduction correlates with tumor progression and aggressiveness. Despite the reduction in the expression of RhoGDI3 in PDAC, we found that RhoB was underexpressed while RhoG was overexpressed, suggesting that cancerous cells preserve their capacity to activate this pathway, thus these cells may be more eager to response to the stimuli needed to proliferate and become invasive unlike normal cells. Surprisingly, we found nuclear localization of RhoGDI3 in non-cancerous pancreatic cell line and normal pancreatic tissue biopsies, which could open the possibility of novel nuclear functions for this protein, impacting gene expression regulation and cellular homeostasis.


PubMed | Hospital General Doctor Manuel Gea Gonzalez and Instituto Nacional Of Rehabilitacion
Type: | Journal: Meta gene | Year: 2015

Cleft lip with or without cleft palate (CL/P) is one of the most common birth defects; it is a multifactorial disease affecting>1/1,000 live births in Europe, and its etiology is largely unknown, although it is very likely genetic and environmental factors contribute to this malformation. Orofacial development is a complex process involving many genes and signaling pathways. Mutations in the gene for the interferon regulatory factor 6 (IRF6) cause a hereditary dominant malformation syndrome including CL/P, and polymorphisms are associated with non-syndromic CL/P (MIM 119530). Five SNPs at the locus with high heterozygosity in Caucasian populations were chosen for the present research due to their very strong association with CL/P. A case-parent trio study was performed using 292 samples from Mexico. Association with the rs1319435-C/C genotype (P=0.02) was found in patients (73) as compared to pseudocontrols (219), while the genotype rs1319435-T/C was related with protection (P=0.041) in the triad design. Significant over-transmission of the G allele for marker rs2235375 (P=0.049) was found. Only the TACGT haplotype was diminished in the affected child, either in single (P=0.0208) or double (P=0.0208) dose. The pairwise analysis showed rs2235543 and rs2235371 were in strong linkage disequilibrium. These results point to a substantial contribution of IRF6 in the etiology of non-syndromic CL/P in a sample of the Mexican population.


PubMed | Hospital General Doctor Manuel Gea Gonzalez
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Apert syndrome (AS) is a frequent acrocephalosyndactyly, with autosomal dominant inheritance. AS has been associated with mutations in fibroblast growth factor receptor 2 (FGFR2), and approximately 99% of cases show 2 of the frequent mutations located in exon IIIa (Ser252Trp or Pro253Arg). The purpose of the present study was to describe the mutations in exon IIIa of FGFR2 in Mexican AS patients and the relationships with clinical features. Exon IIIa of FGFR2 from 6 AS patients was amplified by polymerase chain reaction. Mutations in exon IIIa of the FGFR2 gene were identified by digestion with the restriction endonuclease Bstx1 and polyacrylamide gel electrophoresis. PCR fragments were cloned into the PCR 2.1 vector, and both DNA strands were sequenced using the T7 promoter and M13 universal cloning region oligonucleotides. Sequence alignment was performed using the MEGA software version 5. The patients major clinical features included craniosynostosis, hypertelorism, proptosis, otitis media, midfacial hypoplasia, rhizomelic shortening, and hyperhidrosis. Mutation S252W was present in 4 patients, while the other 2 patients had P253R. In conclusion, either S252W or P253R mutations were present independently in AS patients; however, the 2 mutations were not found together. None of the clinical features were associated with any of the mutations, suggesting that other mutations may be involved in the development of this syndrome.

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