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Leon D.A.,Universitary Hospital Dr Peset | Gayete J.V.,Universitary Hospital Dr Peset | Munoz C.A.,Cytology Unit | Batalla C.C.,University of Valencia | And 2 more authors.
Journal of Reproduction and Contraception | Year: 2014

Objective: To study the identification of the cause of specific sperm abnormalities. Methods: Two adult men with specific alterations in sperm morphology causing 100% immobility were included in this study. The study of sperm used: transmission electron microscopy (both patients); apoptotic markers, DNA fragmentation test and fluorescence in-situ hybridization (patient 1) and immunocytochemistry study of sperm flagellum using anti-β tubulin antibodies and ciliary activity test (patient 2). Results: Increased DNA fragmentation (52.6%) and apoptosis biomarkers were detected in patient 1, and loss of the central pair of microtubules in patient 2 ('9+0' axoneme); the nasal ciliary activity was normal. Conclusion: Results suggest an apoptotic origin of the abnormalities in the sperm from patient 1 and dysplasia of the fibrous sheath in patient 2. © 2014 The Editorial Board of Journal of Reproduction and Contraception.

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