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České Budějovice, Czech Republic

Stiasny K.,Medical University of Vienna | Aberle J.H.,Medical University of Vienna | Chmelik V.,Hospital Ceske Budejovice a.s. | Karrer U.,University of Zurich | And 2 more authors.
Journal of Clinical Virology

Background: Tick-borne encephalitis (TBE) is the most important arbovirus disease in parts of Europe and Asia. Its laboratory diagnosis depends on the detection of specific IgM antibodies which can be impeded by (1) long-time persistence of IgM antibodies after infection, (2) vaccine-induced IgM antibodies, and (3) cross-reactive IgM antibodies from other flavivirus infections. Objectives: To assess the extent of interference factors in the serodiagnosis of TBE that might lead to the false positive assignment of a recent infection. Study design: We quantified TBE virus-specific IgM and IgG antibodies in sera collected at different time points from cohorts of (1) 61 TBE patients, (2) 131 TBE vaccinees, and (3) 42 patients with recent dengue or West Nile virus infections. Results: All of the TBE patients were IgM- and IgG-positive upon hospitalization and 87% of acute TBE sera had IgM antibody titers of >500 Arbitrary Units (AU). These titers rapidly declined and only 16% of TBE patients had low IgM titers ≥9. months after infection. Vaccine-induced as well as flavivirus cross-reactive IgM antibodies were rarely detectable and of low titer. Conclusions: Most of the potential problems of TBE serodiagnosis can be resolved by the quantification of IgM antibodies in a single serum sample taken upon hospitalization. High IgM values (>500. AU in our assay) are indicative of a recent infection. Lower IgM values, however, may require the analysis of a follow-up sample and/or a specific neutralization assay to exclude the possibilities of IgM persistence, vaccine-induced IgM antibodies or heterologous flavivirus infections. © 2012 Elsevier B.V. Source

Votavova H.,Institute of Hematology and Blood Transfusion | Dostalova Merkerova M.,Institute of Hematology and Blood Transfusion | Fejglova K.,Institute of Hematology and Blood Transfusion | Vasikova A.,Institute of Hematology and Blood Transfusion | And 7 more authors.

Objectives: Maternal smoking has a negative effect on all stages of pregnancy. Tobacco smoke-related defects are well established at the clinical level; however, less is known about molecular mechanisms underlying these pathologic conditions. We thus performed a comprehensive analysis of transcriptome alterations induced by smoking in maternal and fetal cells. Study design: Samples of peripheral blood (PB), placenta (PL), and cord blood (UCB) were obtained from pregnant smokers (n = 20) and gravidas without significant exposure to tobacco smoke (n = 52). Gene expression profiles were assayed by Illumina Expression Beadchip v3 for analysis of 24,526 transcripts. The quantile method was used for normalization. Differentially expressed genes were analyzed in the Limma package and the P-values were corrected for multiple testing. Unsupervised hierarchical clustering was performed using average linkage and Euclidean distance. The enrichment of deregulated genes in biological processes was analyzed in DAVID database. Results: Comparative analyses defined significant deregulation of 193 genes in PB, 329 genes in PL, and 49 genes in UCB of smokers. The deregulated genes were mainly related to xenobiotic metabolism, oxidative stress, inflammation, immunity, hematopoiesis, and vascularization. Notably, functional annotation of the affected genes identified several deregulated pathways associated with autoimmune diseases in the newborns of smokers. Conclusions: The study demonstrated maternal smoking causes significant changes in transcriptome of placental and fetal cells that deregulate numerous biological processes important for growth and development of the fetus. An activation of fetal CYP genes showed a limited ability of the placenta to modulate toxic effects of maternal tobacco use. © 2011 Elsevier Ltd. All rights reserved. Source

Rossnerova A.,Academy of Sciences of the Czech Republic | Spatova M.,Academy of Sciences of the Czech Republic | Pastorkova A.,Academy of Sciences of the Czech Republic | Tabashidze N.,Academy of Sciences of the Czech Republic | And 4 more authors.
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

The aim of this study was to analyze genetic damage in human lymphocytes measured using automated image analysis of micronuclei (MN) in a group of 178 mothers and their newborns from two locations in the Czech Republic. The concentrations of benzo[a]pyrene (B[a]P), particulate matter of aerodynamic diameter <2.5μm (PM2.5) and benzene were measured by stationary monitoring in the winter season of 2008/2009 in the capital city of Prague and in Ceske Budejovice, a regional city in a rural area. The 3-month mean concentration of B[a]P before delivery was lower in Prague in comparison with Ceske Budejovice: 1.9±0.5ng/m 3 vs. 3.2±0.2ng/m 3 (p<0.001). The opposite trend was found for PM2.5 and benzene: 27.0±2.5μg/m 3 and 2.5±0.5μg/m 3 vs. 24.5±0.7μg/m 3 and 2.1±0.8μg/m 3 (p<0.001) for Prague vs. Ceske Budejovice, respectively. The average age of the mothers was 31 years (range, 18-49 years). The frequencies of MN per 1000 binucleated cells were 8.35±3.06 vs. 6.47±2.35 (p<0.001) for mothers from Prague and Ceske Budejovice, respectively, and 2.17±1.32 vs. 3.82±2.43 (p<0.001) for newborns from Prague and Ceske Budejovice, respectively. Other factors, including vitamin intake, exposure to tobacco smoke, body mass index (BMI) before pregnancy, the education level of the mothers and the impact of the mothers' and fathers' ages were analyzed in our study. The results suggest that the different sensitivity of the study groups to various mixtures of carcinogenic pollutants could be affected by significant differences in lifestyle factors. Possible higher genetic damage was analyzed in newborns of smoking mothers, and the birth weight of this group was 7.4% lower (p<0.05) in comparison with the newborns of nonsmoking mothers. No impact of the age of the mothers or fathers on MN frequency in the newborns was observed. © 2011 Elsevier B.V. Source

Veleminsky Jr. M.,University of South Bohemia | Veleminsky Jr. M.,Hospital Ceske Budejovice a.s. | Fuxova A.,Hospital Ceske Budejovice a.s. | Petr S.A.K.,University of South Bohemia | Petr S.A.K.,Hospital Ceske Budejovice a.s.
Neuroendocrinology Letters

The authors present cases on rare but serious complications in pregnancy and Puerperium concerning women with cystic aorta medionecrosis, gallbladder carcinoma and Hodgkin lymphoma. They describe the prognosis of pregnant women and their newborns with oncological and non-oncological malignancies while stressing an individual approach. ©2011 Neuroendocrinology Letters. Source

Sokol D.,Hospital Ceske Budejovice a.s. | Fiedler J.,Hospital Ceske Budejovice a.s. | Chlouba V.,Hospital Ceske Budejovice a.s. | Bombic M.,Hospital Ceske Budejovice a.s. | Priban V.,Hospital Ceske Budejovice a.s.
Acta Neurochirurgica

Background: In this article, we present our experience with such operations performed under local anaesthesia. Methods: From January 1997 to November 2007, there were 387 patients operated on for asymptomatic carotid stenosis. Patient data were retrospectively evaluated. Thirty-day neurological morbidity and mortality from six different subgroups were analysed and compared. The numbers of perioperative transient ischaemic attacks, as well as surgical and other perioperative complications were also evaluated. Results: Overall morbidity and mortality was 1.8% (seven patients). Stroke was noted in 1.3% (five patients). Transitory ischaemic attacks within the first 30 days were observed in 1.6% (six patients). Only those patients who had intraluminal shunt insertion were found to have significantly higher morbidity and mortality. (p=0.000018). Myocardial infarction was observed in 0.5% (two patients), one fatal. Conclusion: We have achieved acceptable morbidity and mortality rates (1.8%) according to the parameters set by previous studies such as Asymptomatic Carotid Atherosclerosis Study and Asymptomatic Carotid Stenosis Trial as well as American Heart Association and European Stroke Organisation guidelines. All surgeries were done under local anaesthesia. Shunts were inserted in 22 cases (5.68%). © 2011 Springer-Verlag. Source

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