Hosokawa Powder Technology Research Institute

Osaka, Japan

Hosokawa Powder Technology Research Institute

Osaka, Japan
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Yang M.,Gifu Pharmaceutical University | Yang M.,Copenhagen University | Yamamoto H.,Gifu Pharmaceutical University | Yamamoto H.,Aichi Gakuin University | And 6 more authors.
European Journal of Pharmaceutical Sciences | Year: 2012

Salmon calcitonin, for the treatment of calcium homeostasis and bone remodeling, was used as a model peptide drug and adsorbed on the surface of biodegradable polymeric poly(dl-lactic-co-glycolic acid) (PLGA) nanospheres. Subsequently, the nanospheres were treated using lyophilizer and loaded onto inhalable carrier using Mechanofusion™ to obtain nanocomposite particles suitable for inhalation. The physicochemical properties and in vitro inhalation properties of the nanocomposite particles were investigated. The pulmonary distribution and pharmacological effect were also evaluated in male Wistar rats. The results showed that the drug loading efficiency of salmon calcitonin on PLGA nanospheres were exceeding 96% (w/w). Inhalation efficiency of the lyophilized PLGA nanospheres was largely improved after they were loaded on the surface of inhalable carrier. Over 50% (w/w) of the lyophilized PLGA nanospheres could be deposited in the alveoli section after intratracheal administration to male Wistar rats, while a rapid elimination rate of the lyophilized nanospheres from the lung was found in pulmonary distribution study. The in vivo pharmacological study showed that the nanocomposite particles exhibited superior hypocalcemic action over salmon calcitonion solution and the lyophilized nanospheres. It suggested that the Mechanofusion™ technique can impart improved inhalation properties to the lyophilized nanospheres for pulmonary delivery of therapeutic peptide drugs. © 2012 Elsevier B.V. All rights reserved.


Yang M.,Gifu Pharmaceutical University | Yang M.,Aichi Gakuin University | Yamamoto H.,Gifu Pharmaceutical University | Yamamoto H.,Copenhagen University | And 6 more authors.
European Journal of Pharmaceutical Sciences | Year: 2012

The aim of this study was to investigate two types of chitosan-modified poly (dl-lactic-co-glycolic acid) (PLGA) nanocomposite particles containing salmon calcitonin for pulmonary delivery, which were obtained using spray drying fluidized bed granulation (Agglomaster™) and dry powder coating techniques (Mechanofusion™), respectively. The physicochemical properties, pulmonary distribution, pulmonary clearance rate as well as in vivo hypocalcemia actions of the two types of nanocomposite particles were investigated. As indicated by scanning electron micrographs, soft matrix nanocomposite particles and soft ordered nanocomposite particles were produced by Agglomaster™ and Mechanofusion™, respectively. Both forms of chitosan-modified PLGA nanocomposite particles exhibited a high inhalation efficiency, i.e. more than 50% of the two types of nanocomposite particles could be deposited in the deep lung of male Wistar rats. However, the chitosan-modified PLGA nanocomposite particles designed by Agglomaster™ exhibited superior properties to those obtained by Mechanofusion™ with respect to the redispersibility of fine particles in aqueous liquid, the pulmonary retention time and pharmacological effects. In addition, compared with non-modified PLGA nanocomposite particles, the chitosan-modified PLGA nanocomposite particles obtained by Agglomaster™ exhibited enhanced pulmonary absorption of salmon calcitonin via the lung. The findings in this study suggest that the spray drying fluidized bed granulation technique is superior to the dry powder coating technique for producing chitosan-modified dry powder formulations containing salmon calcitonin for inhalation. This can be attributed to the avoidance of aggregation of chitosan-modified PLGA nanocomposite particles when using Agglomaster™ rather than Mechanofusion™. © 2012 Elsevier B.V. All rights reserved.


Matsusaka S.,Kyoto University | Wei D.,Kyoto University | Yasuda M.,Kyoto University | Yasuda M.,imp Inc | Sasabe S.,Hosokawa Powder Technology Research Institute
Advanced Powder Technology | Year: 2015

An improved airflow method to measure the distribution of adhesive strength between charged particles and a metal substrate in an external electric field is presented. In this study, toner particles were negatively charged with a corona charger and deposited on the substrate. The substrate with the particles on the surface was mounted in a rectangular air channel with parallel electrodes. Air velocity was increased at a constant rate, and entrained particles were detected by a laser particle monitor. By studying the relationships between particle entrainment efficiency and air velocity, the particle-substrate adhesion was analyzed in detail. It was found that particle adhesion increased with the increase in the initial charge of particles. It was also found that the particle adhesion increased in a vertically downward electric field but decreased in the upward electric field. These experimental results cannot be explained by the Coulomb force in the electric field. Therefore, a theoretical model based on charge transfer in the external electric field was proposed. This model explains the variation of the particle-substrate adhesion by considering the image force arising due to the transferred charges. © 2014 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder Technology Japan. All rights reserved.


Kim Y.-H.,Hanyang University | Jung Y.-G.,Changwon National University | Yoon G.S.,Hanyang University | Moon J.,Samsung | And 4 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2012

Rectangular ceria particles were synthesized using the flash creation method. The influence of the morphology of ceria particles and the surfactant concentration on the removal rate was systematically investigated. These ceria slurries with polymeric surfactant molecules as the passivation agents of Si 3N 4 film, shows an exceptional non-Prestonian behaviors. The non-Prestonian behavior can be attributed to the increase in the contact area of the ceria particles with the SiO 2 film, which is dominated by the morphology of the ceria particles. Force measurements using an atomic force microscope (AFM) at different concentrations of polymeric surfactant molecules was used to identify the interactions between the polymeric molecules and the oxide film and analyze the non-Prestonian behavior of ceria slurry having rectangular abrasives. Copyright © 2012 American Scientific Publishers.


Tahara K.,Aichi Gakuin University | Tahara K.,Nagoya City University | Samura S.,Aichi Gakuin University | Tsuji K.,Aichi Gakuin University | And 6 more authors.
Biomaterials | Year: 2011

Chitosan (CS)-modified poly(d,. l-lactide-co-glycolide) (PLGA) nanospheres (NS) were developed and evaluated for use with a nuclear factor kappa B (NF-κB) decoy oligonucleotide (ODN) oral delivery system in an experimental model of ulcerative colitis (UC). Decoy ODN-loaded PLGA NS were prepared by an emulsion solvent diffusion method, and the physicochemical properties of NS were investigated. CS-modified PLGA NS (CS-PLGA NS) showed positive zeta potential, while unmodified PLGA NS (plain-PLGA NS) were negatively charged. Decoy ODN uptake studies with Caco-2 cells using confocal laser scanning microscopy (CLSM) indicated that CS-PLGA NS were more effectively taken up by the cells than plain-PLGA NS. Decoy ODN-loaded CS-PLGA NS were able to improve the stability of ODN against DNase I or an acidic medium, such as gastric juice. Daily oral administration of CS-PLGA NS in a rat model significantly improved dextran sulfate sodium-induced diarrhea, bloody feces, shortening of colon length, and myeloperoxidase activity. Furthermore, decoy ODN-loaded CS-PLGA NS were specifically deposited and adsorbed on the inflamed mucosal tissue of the UC model rat. These results suggested that CS-PLGA NS provide an effective means of colon-specific oral decoy ODN delivery in UC. © 2010 Elsevier Ltd.


Takenaka K.,Osaka University | Nakajima Y.,Osaka University | Setsuhara Y.,Osaka University | Abe H.,Osaka University | Nogi K.,Hosokawa Powder Technology Research Institute
Ceramic Transactions | Year: 2010

A plasma-enhanced nanoparticle-beam deposition method under vacuum has been developed. The deposition rate is 150 nm/min, which is 1-2 orders higher than that obtained using conventional reactive sputter deposition. Using this method, the surface smoothness of the resultant nanoparticle films is significantly enhanced.

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