Horticultural Sub Academy

Harbin, China

Horticultural Sub Academy

Harbin, China
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Wang H.,Northeast Forestry University | Wang H.,Horticultural Sub Academy | Wang T.,Northeast Forestry University | Wang T.,Horticultural Sub Academy | And 2 more authors.
Journal of Computational and Theoretical Nanoscience | Year: 2015

The objective of this study is to investigate the interference effects of tourism on the structural composition and species diversity of plant communities in Yabuli National Forest Park. We aim to provide some references for sustainable development of tourism and protection of ecological environment in Yabuli National Forest Park. Some study plots were selected from areas at different altitudes which were far from or near the tourist route. Four typical plant communities in Yabuli were selected. The results show that tourism activities would change the structural compositions of plant communities in Yabuli National Forest Park, and alter the original dominant species, especially herbs. Moreover, the species diversity within the community first increases and then decreases along with the increase of interference intensity, and the species diversity is maximized (S = 19) at the interference intensity 2. Thus, for sustainable tourism development in Yabuli, appropriate measures should be taken to alleviate the interference intensity of tourism activities and thus to eliminate its impacts on plant communities in Yabuli National Forest Park. Copyright © 2015 American Scientific Publishers All rights reserved.

Zhao Z.,Nanjing Forestry University | Zhao Z.,Hebei University of Engineering | Wang H.,Horticultural Sub Academy | Du J.,Hebei University of Engineering | And 3 more authors.
Journal of Biobased Materials and Bioenergy | Year: 2016

Studies on forest carbon sinks in the past years mainly focus on regional and national scale forest ecosystems, but ignore the relationships between topographical changes and forest carbon sinks within each component (vegetation, soil, litter) of forest ecosystem. Here we estimated the carbon storages and carbon densities in forest vegetation, soil and litter in Zu Mountains, Hebei Province, North China, using geographic information system (GIS) and the Geostatistical theory. We mapped the spatial distributions, analyzed and discussed the effects of vegetation types and topographical factors on forest carbon storage and carbon density. Results show that (1) Co-kriging (16.59% higher precision) and ordinary kriging interpolations are reliable at estimating forest carbon density, and Circular model was the Optimal one. (2) Total forest carbon storage is 83.92 × 104 t, with 13.25 × 104 t in vegetation, 69.71 × 104 t in soil, and 0.96 × 104 t in litter. Forest average carbon density is 144.34 t/ha, with 22.79 t/ha in vegetation, 119.90 t/ha in soil, and 1.65 t/ha in litter. (3) Spatial distribution maps of co-kriging interpolation show that the forest carbon density is similar to the DEM trend and significantly correlated with forest type, elevation and aspect. Our results suggest that co-kriging interpolation is suitable to estimate the forest carbon storage and carbon density. Forest carbon storage and carbon density in each component in Zu Mountains are both significantly different with forest type and topography. The natural forest protecting projects and scientific tending measurement are beneficial to carbon accumulation. Copyright © 2016 American Scientific Publishers All rights reserved.

Wang H.,Horticultural Sub Academy | Wang H.,Northeast Forestry University | Wang Z.,Heilongjiang Academy of Agricultural science | Yu H.,Northeast Forestry University
Journal of Animal and Veterinary Advances | Year: 2012

Mesenchymal Stem Cells (MSC) are known to have the ability to differentiate into various lineages of mesenchymal tissue. They are widely distributed in a variety of tissues in the body and are also present in the foetal environment. In this study researchers have evaluated the possibility of MSC or cells with MSC-like potency being present in the Umbilical Cord (UC). To research the biological characteristics of rabbit Umbilical Cord Mesenymal Stem Cells (UCMSCs), the methods of immunocytochemistry and RT-PCR were used to detect the surface markers of UCMSCs. Growth curves were drawn in accordance with the cell numbers. To assess the differentiating capacity of UCMSCs, cells were induced to neural cells. These results suggested that the UCMSCs isolated from rabbit owned the similar biological characteristics with the cells separated from other species and the potential of differentiation of UCMSCs may represent an ideal candidate resource for cellular transplant therapy in tissue engineering.© Medwell Journals, 2012.

Xie L.,Horticultural Sub Academy | Wang X.,Horticultural Sub Academy | Peng M.,Northeast Forestry University | Meng F.,Northeast Forestry University | And 5 more authors.
Biochemical Systematics and Ecology | Year: 2014

Hot pepper (Capsicum annuum L.) is used as an important crop all over the world. To detect the differential genes of hot pepper mutants, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their on-ground controls (W1 and W2), were firstly analyzed using amplified fragment length polymorphisms (AFLPs) markers. In this study, 37 out of random-selected 256 primer combinations could amplify clear high-intensity, reproducible and relatively high polymorphism bands. Accordingly, 287 different bands based on presence/absence or differences in intensity were identified and sequenced, 68 differential genes were classified functionally into different groups using Gene Ontology (GO) terms. According to GO annotation, 68 expressed sequence tags (ESTs) were homologous with known genes, which mainly included transcription factors, protein kinases, metabolic pathways, cellular processes and stress injury. In conclusion, these findings could provide the fundamental basis for further research in breeding hot pepper with high yield or the mechanism of space inducement. © 2014 Elsevier Ltd.

Xie L.,Horticultural Sub Academy | Guo Y.,Horticultural Sub Academy | Meng F.,Northeast Forestry University | Dang H.,Northeast Forestry University | And 4 more authors.
5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 | Year: 2011

t- To investigate the characteristics of change of hot pepper (Capsicum annuum L.) generation from seeds carried by recoverable satellite, we observed the ultrastructure of leaf mesophyll cells and pollen in the first generation (KL06-102hd) and ground control (KL06-102) with an electron microscope (EM). Seven primers amplified a total of thirty-six sites in genomic DNA of KL06-102 and KL06-102hd by random amplified polymorphic DNA (RAPD), of which eighteen sites were polymorphic. The results showed that the ultrastructure of the leaf mesophyll cells and the pollen grains in KL06-102hd changed. Perhaps these results suggested that space environment can induce the changes of hot pepper plant. © 2011 IEEE.

Yu P.,Zhejiang University | Jiang S.,Shanghai Academy of Agricultural science | Wang X.,Horticultural Sub Academy | Bai S.,Zhejiang University | Teng Y.,Zhejiang University
European Journal of Horticultural Science | Year: 2016

Pyrus ussuriensis Maxim. cultivars have long been considered to be directly derived from wild P. ussuriensis, which is distributed in northeastern China. However, recent studies have revealed that P. ussuriensis cultivars are similar to Pyrus pyrifolia, which is extensively cultivated in China. To clarify the origin of cultivated P. ussuriensis, 12 primers of retrotransposon-based sequence-specific amplification polymorphism marker were used to analyze the genetic relationships among 92 Pyrus accessions, including cultivated P. pyrifolia, cultivated and wild P. ussuriensis, and a few Occidental species. We obtained 1,143 scorable fragments using 12 primer combinations, of which 871 were polymorphic (i.e., 76.20%). A dendrogram produced using Dice similarity coefficients and the unweighted pair group method with arithmetic mean indicated that wild and cultivated P. ussuriensis form a group parallel to the P. pyrifolia group, in which a few P. ussuriensis cultivars are scattered and further divided into independent subgroups. A Bayesian model-based analysis of population structures revealed that P. ussuriensis cultivars consist of two major genepools, with one originating from wild P. ussuriensis and the other from cultivated P. pyrifolia. Our results suggest that cultivated P. ussuriensis originated from an interspecific hybridization between P. ussuriensis and P. pyrifolia. Our findings not only provide new insights into the origin of cultivated P. ussuriensis, but may contribute to the improvement of P. ussuriensis cultivars. © ISHS 2016.

Wang Y.S.,ShenYang Agricultural University | Wang Y.S.,Qiqihar University | Liu Z.Y.,ShenYang Agricultural University | Li Y.F.,Horticultural Sub Academy | And 3 more authors.
Genetics and Molecular Research | Year: 2013

Artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. In the present study, genetic analysis showed that a single-dominant gene, Re (red leaf), determines the red leaf trait in ornamental kale. An F2 population consisting of 500 individuals from the cross of a red leaf double-haploid line 'D05' with a white leaf double-haploid line 'D10' was analyzed for the red leaf trait. By combining bulked segregant analysis and sequence-related amplified polymorphism technology, we identified 3 markers linked to the Re/re locus. A genetic map of the Re locus was constructed using these sequence-related amplified polymorphism markers. Two of the markers, Me8Em4 and Me8Em17, were located on one side of Re/re at distances of 2.2 and 6.4 cM, whereas the other marker, Me9Em11, was located on the other side of Re/re at a distance of 3.7 cM. These markers could be helpful for the subsequent cloning of the red trait gene and marker-assisted selection in ornamental kale breeding programs. © FUNPEC-RP.

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