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Wang Y.S.,ShenYang Agricultural University | Wang Y.S.,Qiqihar University | Liu Z.Y.,ShenYang Agricultural University | Li Y.F.,Horticultural Sub Academy | And 3 more authors.
Genetics and Molecular Research | Year: 2013

Artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. In the present study, genetic analysis showed that a single-dominant gene, Re (red leaf), determines the red leaf trait in ornamental kale. An F2 population consisting of 500 individuals from the cross of a red leaf double-haploid line 'D05' with a white leaf double-haploid line 'D10' was analyzed for the red leaf trait. By combining bulked segregant analysis and sequence-related amplified polymorphism technology, we identified 3 markers linked to the Re/re locus. A genetic map of the Re locus was constructed using these sequence-related amplified polymorphism markers. Two of the markers, Me8Em4 and Me8Em17, were located on one side of Re/re at distances of 2.2 and 6.4 cM, whereas the other marker, Me9Em11, was located on the other side of Re/re at a distance of 3.7 cM. These markers could be helpful for the subsequent cloning of the red trait gene and marker-assisted selection in ornamental kale breeding programs. © FUNPEC-RP.

Wang H.,Horticultural Sub Academy | Wang H.,Northeast Forestry University | Wang Z.,Heilongjiang Academy of Agricultural science | Yu H.,Northeast Forestry University
Journal of Animal and Veterinary Advances | Year: 2012

Mesenchymal Stem Cells (MSC) are known to have the ability to differentiate into various lineages of mesenchymal tissue. They are widely distributed in a variety of tissues in the body and are also present in the foetal environment. In this study researchers have evaluated the possibility of MSC or cells with MSC-like potency being present in the Umbilical Cord (UC). To research the biological characteristics of rabbit Umbilical Cord Mesenymal Stem Cells (UCMSCs), the methods of immunocytochemistry and RT-PCR were used to detect the surface markers of UCMSCs. Growth curves were drawn in accordance with the cell numbers. To assess the differentiating capacity of UCMSCs, cells were induced to neural cells. These results suggested that the UCMSCs isolated from rabbit owned the similar biological characteristics with the cells separated from other species and the potential of differentiation of UCMSCs may represent an ideal candidate resource for cellular transplant therapy in tissue engineering.© Medwell Journals, 2012.

Xie L.,Horticultural Sub Academy | Wang X.,Horticultural Sub Academy | Peng M.,Northeast Forestry University | Meng F.,Northeast Forestry University | And 5 more authors.
Biochemical Systematics and Ecology | Year: 2014

Hot pepper (Capsicum annuum L.) is used as an important crop all over the world. To detect the differential genes of hot pepper mutants, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their on-ground controls (W1 and W2), were firstly analyzed using amplified fragment length polymorphisms (AFLPs) markers. In this study, 37 out of random-selected 256 primer combinations could amplify clear high-intensity, reproducible and relatively high polymorphism bands. Accordingly, 287 different bands based on presence/absence or differences in intensity were identified and sequenced, 68 differential genes were classified functionally into different groups using Gene Ontology (GO) terms. According to GO annotation, 68 expressed sequence tags (ESTs) were homologous with known genes, which mainly included transcription factors, protein kinases, metabolic pathways, cellular processes and stress injury. In conclusion, these findings could provide the fundamental basis for further research in breeding hot pepper with high yield or the mechanism of space inducement. © 2014 Elsevier Ltd.

Xie L.,Horticultural Sub Academy | Guo Y.,Horticultural Sub Academy | Meng F.,Northeast Forestry University | Dang H.,Northeast Forestry University | And 4 more authors.
5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 | Year: 2011

t- To investigate the characteristics of change of hot pepper (Capsicum annuum L.) generation from seeds carried by recoverable satellite, we observed the ultrastructure of leaf mesophyll cells and pollen in the first generation (KL06-102hd) and ground control (KL06-102) with an electron microscope (EM). Seven primers amplified a total of thirty-six sites in genomic DNA of KL06-102 and KL06-102hd by random amplified polymorphic DNA (RAPD), of which eighteen sites were polymorphic. The results showed that the ultrastructure of the leaf mesophyll cells and the pollen grains in KL06-102hd changed. Perhaps these results suggested that space environment can induce the changes of hot pepper plant. © 2011 IEEE.

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