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Agut H.,University Pierre and Marie Curie | Agut H.,French Institute of Health and Medical Research | Agut H.,Hopitaux Universitaires la Pitie Salpetriere Charles Foix
Bulletin de l'Academie Nationale de Medecine | Year: 2014

Ebola virus belongs to the Filoviridae family and includes several species, including the Ebola Zaire virus, responsible for an initial outbreak of hemorrhagic fever in 1976 and the current epidemic of West Africa. Fruit bats of tropical forests are the likely reservoir of this enveloped RNA virus which, following the incidental infection of a human being, is efficiently transmitted from man to man. Ebola virus exhibits a wide cell and tissue tropism and causes a deleterious acute infection leading to severe sepsis burdened with a heavy mortality. During the acute phase, the virus is very abundant in biological fluids where it can temporarily retain its infectivity for several days after death. Virological diagnosis is done mainly by RT-PCR on a blood sample and needs to be practiced in high security conditions. The pathophysiology of the infection allows to consider curative treatments and vaccination which are urgently needed.


Compain F.,University Pierre and Marie Curie | Frangeul L.,Institute Pasteur Paris | Drieux L.,University Pierre and Marie Curie | Drieux L.,Hopitaux Universitaires la Pitie Salpetriere Charles Foix | And 5 more authors.
Antimicrobial Agents and Chemotherapy | Year: 2014

We report here the complete nucleotide sequence of two IncR replicons encoding multidrug resistance determinants, including β-lactam (bla DHA-1, blaSHV-12), aminoglycoside (aphA1, strA, strB), and fluoroquinolone (qnrB4, aac6′-1b-cr) resistance genes. The plasmids have backbones that are similar to each other, including the replication and stability systems, and contain a wide variety of transposable elements carrying known antibiotic resistance genes. This study confirms the increasing clinical importance of IncR replicons as resistance gene carriers. Copyright © 2014, American Society for Microbiology. All Rights Reserved.


Abrao E.P.,University Pierre and Marie Curie | Burrel S.,University Pierre and Marie Curie | Desire N.,University Pierre and Marie Curie | Bonnafous P.,University Pierre and Marie Curie | And 5 more authors.
Journal of Medical Virology | Year: 2015

Herpes simplex virus type 2 (HSV-2) is the most common cause of genital ulcer disease worldwide. While the contribution of HSV-2 to acquisition and course of human immunodeficiency virus (HIV) infection has been well described, less attention has been paid to the impact of HIV infection on the variability and the pathophysiology of HSV-2 infection. The goal of the present study was to characterize genotypically and phenotypically HSV-2 strains isolated from 12 patients infected by HIV-1 and from 12 HIV-negative patients. Replication capacity analyses were carried out in Vero cells and full-length nucleotide sequences were determined for glycoproteins B (gB), D (gD), G (gG), thymidine kinase (TK), and DNA polymerase (POL) HSV-2 genes. Sequence alignments and phylogenetic trees were performed. No significant differences were found in terms of replication capacity. The interstrain nucleotide identities of the 3 glycoprotein genes (gB, gC, and gG) ranged from 99.5% to 100% among the 24 HSV-2 strains. The phylogenetic analysis showed no clustering of HSV-2 strains when correlating to the HIV status of the patients. A lower variability was observed for the functional proteins TK and DNA polymerase (98.9% to 100% identity). Genetic analysis of TK evidenced mutations related to acyclovir-resistance in two HSV-2 strains. No specific differences regarding replication capacity and gene sequence were found when comparing HSV-2 strains isolated from patients infected with HIV-1 and HIV-negative patients, suggesting that the virological properties of HSV-2 infection are not influenced by HIV-1 infection among co-infected patients. J. Med. Virol. 87:357-365, 2015. © 2014 Wiley Periodicals, Inc.


Houze S.,Center Hospitalier University Bichat Claude Bernard | Paris L.,Hopitaux Universitaires la Pitie Salpetriere Charles Foix
Revue Francophone des Laboratoires | Year: 2015

Summary Rapid diagnostic tests whose interest lies in their implementation without specific equipment by unskilled personnel have grown significantly over the past fifteen years to malaria diagnosis with detection of specific Plasmodium proteins, mainly PfHRP2 and pLDH. If the detection of PfHRP2 makes a very good sensitivity for the diagnosis of Plasmodium falciparum malaria attacks, research pLDH or aldolase are lower for other species, imposing retain microscopic diagnosis reference. This methodology is now being applied to the diagnosis of lymphatic filariasis as well. At the same time, the detection of specific antibody developed for Chagas disease and visceral leishmaniasis and recently schistosomiasis. These tests have sensitivities and specificities variables, the biologist must know before choosing the reagent that will implement in his laboratory.


Vialatte de Pemille C.,Hopitaux Universitaires la Pitie Salpetriere Charles Foix | Bielle F.,Hopitaux Universitaires Pitie Salpetriere Charles Foix | Mokhtari K.,Hopitaux Universitaires la Pitie Salpetriere Charles Foix | Kerboua E.,Head and Neck Unit | And 2 more authors.
World Neurosurgery | Year: 2016

Intracranial germinoma is a rare primary brain cancer, usually located within the midline and mainly affecting Asian pediatric patients. Interestingly, we report here the peculiar case of a young North-African adult patient suffering from a basal ganglia germinoma without the classical ipsilateral cerebral hemiatrophy associated with this location. © 2016 Elsevier Inc.

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