Pan H.,Jiangnan University |
Liao T.,Hongyunhonghe Research Institute of Fragrance and Flavor Industry |
Liao T.,China Tobacco Yunnan Industrial Co. |
Zhu B.,Hongyunhonghe Research Institute of Fragrance and Flavor Industry |
And 7 more authors.
Journal of the Chemical Society of Pakistan | Year: 2015
Extractions of polyphenols from Phyllanthus emblica L. were performed by supercritical CO2 fluid extraction (SFE). A three-level Box-Behnken factorial design using response surface methodology was applied to optimize the main extraction conditions, including the ratio of the solvent to raw material, pressure, extraction temperature and extraction time on the PEP yields. The results showed that the optimal conditions of the CO2 flow rate was 35.9 l/h, pressure was 90.1 MPa, the extraction temperature was 55°C and the extraction time was 1.1 h. Under optimum conditions, PEP yields of 84.19±0.51 mg GAE/100 g were obtained, which closely matches the predicted value of the yield. Simultaneous determination of nine polyphenol compounds in polyphenols using HPLC with fluorescence detection has been successfully achieved. HPLC analysis indicated that the major polyphenols in the SFE extracts consisted of chlorogenic acid, caffeic acid, syrigin, procyanidin B2, (-)-epicatechin, cinnamic acid, coumaric acid, phlorizin and quercetin, of which procyanidin B2 had the highest content of 231.77 mg/kg.