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Li X.-F.,Shanghai University of Traditional Chinese Medicine | Hu X.-H.,Hongdu Hospital of Traditional Chinese Medicine | Xu H.,Shanghai University of Traditional Chinese Medicine | Zhao Y.-J.,Shanghai University of Traditional Chinese Medicine | And 6 more authors.
WIT Transactions on the Built Environment | Year: 2014

Most publications have shown that Icariin could stimulate bone fracture healing and treat postmenopausal osteoporosis (OP) in ovariectomized animal model. As another novel animal model of OP, there are few publications about the effect of Icariin in osteoprotegerin-deficient mice. Therefore, our goal is to find the effect on bone formation and underlying mechanisms of Icariin in osteoprotegerin (OPG) knockout (KO) mice. We found that Icariin could significantly reverse the bone loss caused by the defect of OPG. We also found Icariin dramatically increased expression of BMP2, BMP4, RUNX2, OC, WNT1, and WNT3a in OPG KO mice. Icariin also dramatically enhanced the expression of AXIN2, DKK1, TCF1, and LEF1 in OPG KO mice, which are the direct target genes of β-catenin pathway. We also found a high dose of Icariin (50 μM) significantly stimulated osteoblast differentiation, demonstrated by the increase of ALP staining and genes expression of Col 1, Runx2, Osterix, Wnt1, Wnt4, and Bmp7 in BMSCs from OPG KO mice. After transfected with adenovirus in BMSCs from β-cateninfx/fx mice, we found that Icariin stimulated the differentiation of osteoblast by activating the pathway of Wnt/ β-catenin. Together, our findings demonstrate that Icariin significantly inhibits OPG-deficient-induced bone loss through the activation of Wnt/β-catenin signaling. © 2014 WIT Press. Source


Tu S.Q.,Hongdu Hospital of Traditional Chinese Medicine
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2012

To study the curative effects of close nail-pry reduction and internal fixation with hollow screws for treatment of linguiform calcaneus fracture. From May 2006 to October 2009,32 patients (35 feet) with linguiform calcaneus fracture were treated by close nail-pry reduction and internal fixation with hollow screws, including 23 males and 9 females ranging in age from 25 to 46 years, with a mean of 37.6 years. According to Paley classification, 3 cases were Paley II a, and 29 cases were Paley II b. All cases were close fractures. The time from injury to operation was 3 to 10 days after most swelling subsided. Böhler angle and Gissane angle were measured by X-ray before and after operation. The therapeutic effect was assessed according to ZHANG Tie-liang's foot score. All the patients were followed-up for 6 to 18 months, with a mean of 12 months. All fractures gained bone healing. The time of fracture healing averaged 12 months. The fractures healed completely and no infection occurred. According to ZHANG Tie-liang's foot scale, the postoperative function was excellent in 18 feet, good in 10 feet, moderate in 5 feet and poor in 2 feet. The Böhler angle and Gissane angle were significant improved after treatment (P < 0.01). The surgical method of close nail-pry reduction and internal fixation with hollow screws for treatment of linguiform calcaneus fracture can regain the foot function, with minimal injury, fewer complications, earlier recovery and lower costs. Source


Huang X.-H.,Shanghai JiaoTong University | Liu N.-J.,Hongdu Hospital of Traditional Chinese Medicine | Dai L.-H.,Shanghai JiaoTong University | Ma K.,Shanghai JiaoTong University
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2016

Objective: To observe the effects of intrathecal injection of CXCR4 specific antagonist AMD3100 on rats of spared nerve injury (SNI) model. Methods: A total of 60 male SD rats were randomly divided into 3 groups, i.e. sham group (n=10), control group (n=25), and AMD3100 group (n=25). SNI was adopted to establish the neuropathic pain model. Rats of AMD3100 group underwent continuous intrathecal injection of AMD3100 (1μg per mouse, once a day for two weeks), while rats of sham group and control group underwent intrathecal injection of the same volume of normal saline. The paw withdrawal threshold (PWT) under mechanical stimulus of affected extremities of rats was measured by Von-Frey filaments. The expression of glial fibrillary acidic protein (GFAP) in the hippocampus was detected by Western blotting and immunofluorescence 14, 21, 28, and 35 d after first administration. Results: Compared with sham group, PWT of control group significantly decreased after SNI operation and the expression of GFAP in the hippocampus increased. The continuous intrathecal injection of AMD3100 significantly increased the PWT of rats and down-regulated the expression of GFAP in the hippocampus. Conclusion: The intrathecal injection of AMD3100 can down-regulate the expression of GFAP in the hippocampus of rats of SNI model and effectively alleviate symptoms of neuropathic pain of rats. © 2016, Editorial Department of Journal of Shanghai Second Medical University. All right reserved. Source


Fan S.-Y.,Nanchang University | Huang K.-D.,Hongdu Hospital of Traditional Chinese Medicine | Tang Q.-M.,Hongdu Hospital of Traditional Chinese Medicine | Huang Z.-R.,Hongdu Hospital of Traditional Chinese Medicine | And 3 more authors.
Chinese Journal of Inorganic Chemistry | Year: 2014

Fe3O4/P(N-isopropylacrylamide-co-acrylamide) [P(NIPAAm-co-Am)], magnetic thermosensitive composite particles were prepared by emulsifier free polymerization. The structure and morphology of the as-synthesized composite particles were characterized by using TEM. and the magnetic and thermosensitive properties were investigated by Vibrating Sample Magnetometer (VSM) and Dynamic Light Scattering (DLS). In addition. the heat generation ability of the composite particles was investigated under alternating magnetic field. The results show that Fe3O4 nanoparticles are coated by a thermosensitive P (NIPAAm-co-Am) with an LCST about 40°C. The composite particles exhibit relatively high magnetic property and heat generation ability. Furthermore. the heat generation ability of the composite particles under alternating magnetic field can be adjusted by controlling the concentration of composite particles and/or the magnetic field. Source


Fan S.-Y.,Nanchang University | Huang K.-D.,Hongdu Hospital of Traditional Chinese Medicine | Tang Q.-M.,Hongdu Hospital of Traditional Chinese Medicine | Huang Z.-R.,Hongdu Hospital of Traditional Chinese Medicine | And 2 more authors.
Gongneng Cailiao/Journal of Functional Materials | Year: 2013

Research on the magnetic thermo-sensitive composite microspheres for controlled release drug carrier was meaningful. Magnetocaloric effect was used to heat the Fe3O4/P (NIPAAm-co-Am) composite microspheres, and the heat generation abilities of the composited microspheres in alternation magnetic field (AMF) were investigated in the present work. Results show the resultant Fe3O4/P (NIPAAm-co-Am) composite microspheres exhibite excellent superparamagnetic and high heating generation ability. The temperature of the composite microspheres was raised after exposed to AMF, which can stimulate the shrinks of P (NIPAAm-co-Am). Furthermore, the temperature raised after exposed to AMF can be adjusted by controlling the concentration of composite microspheres, the magnetic intensity and the frequency of the AMF. Source

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