Yang R.,Michigan State University |
Song B.,Michigan State University |
Sun Z.,Michigan State University |
Lai K.W.C.,City University of Hong Kong |
And 5 more authors.
Nanomedicine: Nanotechnology, Biology, and Medicine | Year: 2015
We present the nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis and mimic the effect of pathophysiological modulation of intercellular adhesion. Nanosurgery successfully severs the intermediate filament bundles and disrupts cell-cell adhesion similar to the desmosomal protein disassembly in autoimmune disease, or the cationic modulation of desmosome formation. Our nanomechanical analysis revealed that adhesion loss results in a decrease in cellular stiffness in both cases of biochemical modulation of the desmosome junctions and mechanical disruption of intercellular adhesion, supporting the notion that intercellular adhesion through intermediate filaments anchors the cell structure as focal adhesion does and that intermediate filaments are integral components in cell mechanical integrity. The surgical process could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. From the Clinical Editor: This team of authors performed nanosurgery on the cytoskeleton of live cells using AFM based nanorobotics to achieve adhesiolysis, and mimic the effect of pathophysiological modulation of intercellular adhesions. This method could potentially help reveal the mechanism of autoimmune pathology-induced cell-cell adhesion loss as well as its related pathways that lead to cell apoptosis. © 2015 Elsevier Inc.
Fang Y.,City University of Hong Kong |
Iu C.Y.Y.,Hong Kong Baptist University |
Lui C.N.P.,Hong Kong Baptist University |
Zou Y.,Hong Kong Baptist University |
And 5 more authors.
Scientific Reports | Year: 2014
Glutamate-mediated neurodegeneration resulting from excessive activation of glutamate receptors is recognized as one of the major causes of various neurological disorders such as Alzheimer's and Huntington's diseases. However, the underlying mechanisms in the neurodegenerative process remain unidentified. Here, we investigate the real-time dynamic structural and mechanical changes associated with the neurodegeneration induced by the activation of N-methyl-D-aspartate (NMDA) receptors (a subtype of glutamate receptors) at the nanoscale. Atomic force microscopy (AFM) is employed to measure the three-dimensional (3-D) topography and mechanical properties of live SH-SY5Y cells under stimulus of NMDA receptors. A significant increase in surface roughness and stiffness of the cell is observed after NMDA treatment, which indicates the time-dependent neuronal cell behavior under NMDA-mediated neurodegeneration. The present AFM based study further advance our understanding of the neurodegenerative process to elucidate the pathways and mechanisms that govern NMDA induced neurodegeneration, so as to facilitate the development of novel therapeutic strategies for neurodegenerative diseases.
Hong Kong Productivity Council | Date: 2013-06-07
An electric vehicle charger includes a DC/DC converter and control circuits. The DC/DC converter includes an inverter module; a transformer module connected to the inverter module; and a converter module connected to the transformer module. The control circuits includes a multi-loop feedback control system connected to the converter module; and gate driving circuits connected to the multi-loop feedback control system and the inverter module. The inverter module includes an IGBT bridge. The transformer module includes a transformer. The converter module includes a diode rectifier bridge.
Hong Kong Productivity Council | Date: 2014-02-17
The present application is directed to a method of waterless processing of textile materials using supercritical fluid, including the use of supercritical fluid to dye the textile materials, the use of supercritical fluid for a pre-processing procedure of cleansing the textile materials, and the use of supercritical fluid to wash off unfixed dyes and cleansing of the textile materials and to perform the post-processing procedure of adding functional materials after the use of supercritical fluid to dye the textile materials. The present application provides an integrated technology of pre-processing, dyeing and post-processing of textile materials using supercritical fluid. At the same time, cleansing and dyeing are performed which effectively raises the efficiency.
Whittington C.M.,C.M. Whittington and Assoc. Ltd. |
Whittington C.M.,C.M. Whittington and Assoc. Pty Ltd. |
Lo W.Y.,Hong Kong Productivity Council |
Yau M.Y.,Hong Kong Productivity Council
Transactions of the Institute of Metal Finishing | Year: 2016
High deposition rates and low internal stress of the deposit from nickel sulphamate electrolytes without the use of addition agents, offset the greater cost compared to the conventional Watts nickel solution. Chloride or bromide ions can be added to the electrolyte to improve conductivity and aid dissolution of the nickel anode with little to no anode polarisation. Anodes can be either sulphur activated or pure nickel forms. These choices have been studied for a high speed nickel sulphamate solution used in reel-to-reel plating of connector strip for the electronics industry. The results provide guidance about the influence of halides and anode form on maintaining consistently low tensile stress, while presenting the opportunity for power savings and reducing the need for regular anode cleaning to remove insoluble residue from sulphur activated nickel. Over 6 years of industrial practice, pure electrolytic nickel anodes with nickel chloride added to the electrolyte have provided cost effective operating conditions combined with satisfactory deposit properties in more than 30 reel-to-reel plating lines in Asia. © 2016 Institute of Materials Finishing.