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Islamabad, Pakistan

Gulfraz M.,Pmas Arid Agriculture University | Iftikhar F.,Honey bee section | Imran M.,Pmas Arid Agriculture University | Zeenat A.,Honey bee section | And 2 more authors.
International Journal of Food Science and Technology | Year: 2011

Antibacterial activities of various honey samples were assessed by using clinical isolates like S. aureus (Gram-positive), E. coli, P. aeruginosa and K. pneumonia (Gram-negative). It was observed that acacia and citrus honey has inhibited growth of all bacterial strains as compared to standard antibiotics (Gentamicine). Inhibition zones for S. aureus (27.4 ± 0.5 mm), E. coli (26.5 ± 0.7 mm), K. pneumonia (24.4 ± 0.5 mm) and P. aeruginosa (22.4 ± 0.2 were observed. Minimum inhibitory concentration of S. aureu (0.068 mg mL-1), E. coli (0.072 mg mL-1), P. aeruginosa (0.084 mg mL-1) and K. pneumonia (0.085 mg mL-1) was obtained for various types of honey samples. Pronounced antibacterial activities as well as standard values of various quality parameters of honey samples are scientifically proven for its use in traditional medicine since ancient time throughout the world. © 2011 The Authors. International Journal of Food Science and Technology © 2011 Institute of Food Science and Technology. Source


Gulfraz M.,Pmas Arid Agriculture University | Iftikhar F.,Honey bee section | Raja S.,Honey bee section | Asif S.,Pmas Arid Agriculture University | And 3 more authors.
African Journal of Biotechnology | Year: 2010

Forty samples of different honey types (Acacia, Ziziphus, Brassica and Citrus) were collected from different areas of Pakistan and analyzed for moisture, pH, total acidity, ash, electrical conductivity, hydroxymethylfurfural (HMF), sucrose, total sugars, invert sugar, protein, proline contents as well as macro and micro elements. The variation in composition of honey samples was observed due to different types of flora. Higher pH (6.56 ± 0.05) was observed for Ziziphus honey, acidity (45.0 ± 2.35 mg/kg) for Citrus, moisture (36.8 ± 1.8%) for Brasica and HMF (32.7 ± 0.49 mg/kg) for Acacia. Whereas, higher concentrations of proline (2.1 ± 0.04 mg/kg) and invert sugar (0.38 ± 0.1%) for Citrus honey and protein (16.5 ± 1.5 g/100g) for Acacia honey were observed. Likewise, a significant level (P < 0.05) of ash, electric conductivity, sucrose, total sugar as well as macro and micro elements was also found in these honey types. Different formulations of honey has significantly inhibited growth of pathogenic microorganisms, Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger when compared to control group, which is an evidence that honey is a therapeutic agent being used since ancient time throughout the world. © 2010 Academic Journals. Source

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