Homoeopathic Pharmacopoeia Laboratory

Ghāziābād, India

Homoeopathic Pharmacopoeia Laboratory

Ghāziābād, India
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Tiwari A.K.,UP Council of Sugarcane Research | Khan M.S.,Konkuk University | Kumar P.,North Eastern Regional Institute of Science and Technology | Tiwari A.,Homoeopathic Pharmacopoeia Laboratory
3 Biotech | Year: 2017

The typical phytoplasma symptoms of little leaf, yellowing, chlorosis, witches’ broom, and stunting growth were observed on Acalypha indica plants during the field survey conducted at Lucknow and surrounding districts in year 2015–2016. To confirm the association and possibility of phytoplasma etiology, PCR assays were performed using universal primer pairs (P1/P6) and nested primer pairs (R16F2n/R2) in a total of five diseased samples along with control. A ~1.2 Kb amplicon was observed in nested PCR assay in diseased sample; however, no band was observed in control sample. The positive amplicons were sequenced for 16S rDNA and used for the virtual RFLP analysis and phylogenetic studies. BLASTn search showed 99–100% sequence identities with the ‘Candidatus phytoplasma asteris’ members (16SrI group) and phylogenetic analysis showed closest relationship with member of 16SrI group. The virtual RFLP assigned it as a member of 16SrI-B subgroup. This is the first record of phytoplasma association of ‘Ca. P. asteris’ subgroup B with A. indica in the world. © 2017, Springer-Verlag Berlin Heidelberg.

Chauhan A.,Gurukul Kangri University | Chauhan G.,Gurukul Kangri University | Gupta P.C.,Homoeopathic Pharmacopoeia Laboratory | Goyal P.,Gurukul Kangri University | Kaushik P.,Gurukul Kangri University
Indian Journal of Pharmacology | Year: 2010

The present study was conducted to evaluate the possible antibacterial activity of Anabaena extracts. Anabaena was isolated from a natural source and cultured in vitro. after suitable growth, cyanobacterial culture was harvested using different solvents. Extracts, thus prepared, were evaluated for their antibacterial potential by agar-well diffusion assay against bacterial species of clinical significance. MIC values were determined further to check the concentration ranges for significant inhibition. HPTLC analysis was done to separate the components of active crude extract in an attempt to identify the bio-active chemical entity. Methanol extract exhibited more potent activity than that of hexane and ethyl acetate extracts. No inhibitory effect was found against Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae. Staphylococcus aureus required about 256 μg/ml of the crude methanol extract for effective inhibition. HPTLC evaluation at 254 nm was performed for the separation of a complex mixture of the methanol extract. The results provide evidence that Anabaena sp. extracts might indeed be potential sources of new antibacterial agents.

Kumar G.,Crossing tech | Verma A.,Crossing tech | Joshi P.,Homoeopathic Pharmacopoeia Laboratory | Arya A.,Crossing tech
Medicinal Plants | Year: 2013

Biologically synthesized silver nanoparticles are being widely used in the field of medicine. This report focuses on extracellular biosynthesis of silver nanoparticles using leaf extracts of four commonly occurring weed. The synthesis of silver nanoparticles was detected by changing colour of plant leaf extracts from green to brown after treatment with AgNO3 and UV-Vis spectrophotometric analysis. These phyto-synthesized silver nanoparticles were tested for their antibacterial activity using agar well diffusion method. The test bacteria were E. coli, Staphylococcus aureus and Bacillus sp. Among all the test organisms Bacillus sp. followed by Staphylococcus aureus were found to be susceptible to all the silver nanoparticles with maximum inhibition by Sonchus asper whereas E. coli was least affected. The results suggest use of phytoextracts as an easy alternative for silver nanoparticles synthesis over the conventional methods.

Tiwari L.,Homoeopathic Pharmacopoeia Laboratory | Pande P.C.,Kumaon University
Asian Agri-History | Year: 2010

The Himalayas have a great diversity of plants and other natural resources. Himalayan people have close relationship with nature for their basic needs such as food, fuel, fodder, and medicine. For health care, they use their own medicine system, which is based on the ancient cultural traditions. The Himalayan people believe that displeasure of local gods is the cause of diseases. They use "magico-religious" practices and natural therapies to treat diseases. The Himalayan Medicine System is interesting and is intimately connected with Ayurveda. This article discusses the relationship of Himalayan Medicine System and Ayurveda and also describes the concept of Himalayan therapies for incurable diseases.

Tiwari L.,Homoeopathic Pharmacopoeia Laboratory | Tiwari L.,Kumaon University | Pande P.C.,Homoeopathic Pharmacopoeia Laboratory | Pande P.C.,Kumaon University
Asian Agri-History | Year: 2011

Central Himalaya covers the state of Uttarakhand in North India. Livestock occupies a very important place in human life in Uttarakhand Himalaya and is an integral part of agriculturebased economy of the state. More than 70% of the rural population of Uttarakhand Himalaya depends upon animals for their economical needs. Majority of the families adopted cattle and buffalo livestock rearing pattern because these animals were thought to provide direct/ indirect income through sale of milk, domestic consumption of milk, and use of dung as farmyard manure. The present paper deals with the traditional animal husbandry practices of Uttarakhand Himalaya.

PubMed | Homoeopathic pharmacopoeia laboratory
Type: Journal Article | Journal: Ancient science of life | Year: 2012

Madicago sativa Linn, commonly known as Alfalfa, is a tonic plant rich in proteins, minerals, enzymes and vitamins, Bulk quantity of the whole plant is required in the pharmaceutical industries especially in homoecopathic pharmacies, Hence, there is a great need to cultivate this plant for sustained supply of the drug. Use of good and adequate phosphatecontaining farm yard manure, timely irrigation and appropriate spacing between plants results in good yield.

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