Holy Family Maternity Hospital

Warsaw, Poland

Holy Family Maternity Hospital

Warsaw, Poland
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PubMed | Holy Family Maternity Hospital, Institute of Tropical Medicine and Military Institute of Medicine
Type: | Journal: Advances in experimental medicine and biology | Year: 2016

The article presents the results of 11-year study (2005-2015) of Gram-negative bacteria responsible for pneumonia in 2033 mechanically ventilated patients hospitalized in Intensive Care Unit. Of 8796 biological samples, consisting mainly of bronchial aspirate (97.9%), 2056 bacterial strains were isolated and subjected to identification. VITEK 2 was used to determine drug susceptibility (classified according to the EUCAST criteria). ESBL, MBL and KPC-producing strains were identified by means of phenotypic methods using appropriate discs. The findings were that the predominant bacteria responsible for infections consisted of Enterobacteriaceae (42.0%), Acinetobacter baumannii (37.2%), Pseudomonas aeruginosa (16.1%), and Stenotrophomonas maltophila (4.7%). We observed a rise in the number of bacteria causing pneumonia throughout the study period, especially in S. maltophila and Enterobacteriaceae ESBL (+). Gram-negative bacilli were 100% susceptible to colistin, apart from naturally resistant strains such as Proteus mirabilis, Serratia marcescens, whereas Enterobacteriaceae ESBL (+) were susceptible to imipenem and meropenem. Acinetobacter baumannii strains exhibited the lowest drug susceptibility. In conclusion, we report an increase in the prevalence of pneumonia associated with Gram-negative bacteria in mechanically ventilated intensive care patients. Colistin remains the most effective drug against the majority of Gram-negative bacteria. Therapeutic problems are common in the course of treatment of Acinetobacter baumannii infections.


PubMed | Holy Family Maternity Hospital, Institute of Tropical Medicine, Military Institute of Medicine and Polish National Medicines Institute
Type: | Journal: Advances in experimental medicine and biology | Year: 2016

The article presents the prevalence of Neisseria meningitidis carriage with the identification of sero- and genogroups in professional soldiers serving in the Polish Armed Forces. A total of 1246 soldiers from the 10th Armored Cavalry Brigade in witoszw, Poland were examined in the period January-February 2016. Microbiological tests were performed using standard methods (culture, incubation, microscopy, biochemical, and automated identification with VITEK cards). Neisseria meningitidis isolates from carriers were subjected to a slide agglutination test for the identification of serogroups, next bacterial DNA was isolated and genogroups were identified based on the results of PCR. Of the 1246 soldiers tested, 65 were found to be carriers of N. meningitidis. Serogroups of 36 isolates and genogroups of 56 meningococcal isolates were determined. The genogrouping identified the isolates as belonging to group B (n=34; 52.3%), E29 (n=8; 12.3%), C (n=6; 9.2%), Y (n=6; 9.2%), and W (n=2; 3.1%). The primers which were used did not make it possible to determine the genogroup of nine isolates. In conclusion, the overall carrier rate of N. meningitidis amounted to 5.2%, with the serogroup B being predominant, which is similar to that reported in the general population in Poland and Central Europe.

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