Kitahiroshima, Japan
Kitahiroshima, Japan

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Furuta K.,Hokusan Co. | Nagashima S.,Hokkaido University | Inukai T.,Hokkaido University | Masuta C.,Hokkaido University
Plant Pathology Journal | Year: 2017

One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge. © 2017, Korean Society of Plant Pathology.

PubMed | Kochi University, University of Tsukuba, China Institute of Technology, Hokusan Co. and 2 more.
Type: Journal Article | Journal: Die Naturwissenschaften | Year: 2016

Nesting behavior is considered to be an important element of social living in animals. The spider mites belonging to the genus Stigmaeopsis spend their lives within nests produced from silk threads. Several of these species show cooperative sociality, while the others are subsocial. In order to identify the origins of this social behavior, comparisons of nest sizes, nesting behaviors (making nests continuously or separately), and their associated traits (fecal deposition patterns) were made for eight cogeneric Stigmaeopsis species showing various levels of social development. All of these species inhabit bamboo plants (Poaceae). We initially addressed the proximate factor of nest size variation. The variation in nest size of the eight species corresponded well with the variation in dorsal seta sc1 length, suggesting that nest size variation among species may have a genetic basis. The time spent within a nest (nest duration) increased with nest size on the respective host plants. Nest arrangement patterns varied among species showing different sized nests: Large nest builders continuously extended their nests, while middle and small nest-building species built new separate nests, which resulted in different social interaction times among species, and is thought to be closely related to social development. Fecal deposition behaviors also varied among Stigmaeopsis species, suggesting diversity in anti-predatory adaptations. Finally, we discuss how the variation in sociality observed within this genus is likely the result of nest size variation that initially evolved as anti-predator strategies.

Hokusan Co. and Mitsui Chemicals Agro Inc. | Date: 2012-06-08

A method of reducing the contamination amount of mycotoxin in cereals wherein one or more compounds A selected from the group consisting of ammonium salts, primary to quaternary ammonium salts, alkali metal salts, alkaline earth metal salts and polyvalent metal salts of phosphorous acid and phosphite ester are given to the cereals.

Hikosaka S.,Chiba University | Yoshida H.,Chiba University | Goto E.,Chiba University | Tabayashi N.,Hokusan Co. | Matsumura T.,Japan National Institute of Advanced Industrial Science and Technology
Environmental Control in Biology | Year: 2013

The transgenic everbearing strawberry (Fragaria·ananassa Duch. 'HS 138') can be cultivated in a closed plant production system to produce functional proteins that enhance human immune functions. In this study, we investigated the effects of light quality on fruit growth and the concentration of human adiponectin (hAdi) at three mature stages in transgenic strawberry. hAdi plants were exposed to 3 different light qualities (white [W], blue [B], and red [R]) for a 16-h light period under fluorescent lamps during the flowering and harvest stage; fruits were then harvested at three different mature stages (small green, turning white, and mature red). hAdi concentration increased with fruit maturation, and the mature red stage fruit from the R light treatment group had a significantly greater concentration of hAdi on a fresh-weight basis than all other treatments. There was no relationship between hAdi concentration and fruit fresh weight and the number of days from anthesis to harvest. Although the factors that promoted the production of the functional hAdi proteins were not clear in this study, the results suggest that the fruit growth stages of the transgenic strawberry differed in their response to light quality. For hAdi plants, exposure to red light resulted in the greatest level of functional protein production under the tested treatments.

Idemitsu Kosan Co., Japan National Institute of Advanced Industrial Science, Technology and Hokusan Co. | Date: 2016-10-05

The present invention provides an agent for controlling Escherichia coli diarrhea comprising a Shiga toxin as an active ingredient.

Matsuo K.,Japan National Institute of Advanced Industrial Science and Technology | Kagaya U.,Hokusan Co. | Itchoda N.,Hokusan Co. | Tabayashi N.,Hokusan Co. | Matsumura T.,Japan National Institute of Advanced Industrial Science and Technology
Journal of Bioscience and Bioengineering | Year: 2014

Production of pharmaceutical glycoproteins, such as therapeutic antibodies and cytokines, in plants has many advantages in safety and reduced costs. However, plant-made glycoproteins have N-glycans with plant-specific sugar residues (core β-1,2-xylose and α-1,3-fucose) and a Lewis a (Lea) epitope, Galβ(1-3)[Fucα(1-4)]GlcNAc. Because it is likely that these sugar residues and glycan structures are immunogenic, many attempts have been made to delete them. Previously, we reported the simultaneous deletion of the plant-specific core α-1,3-fucose and α-1,4-fucose residues in Lea epitopes by repressing the GDP-d-mannose 4,6-dehydratase (GMD) gene, which is associated with GDP-l-fucose biosynthesis, in Nicotiana benthamiana plants (rGMD plants, renamed to δGMD plants) (Matsuo and Matsumura, Plant Biotechnol. J., 9, 264-281, 2011). In the present study, we generated a core β-1,2-xylose residue-repressed transgenic N.benthamiana plant by co-suppression of β-1,2-xylosyltransferase (δXylT plant). By crossing δGMD and δXylT plants, we successfully generated plants in which plant-specific sugar residues were repressed (δGMDδXylT plants). The proportion of N-glycans with deleted plant-specific sugar residues found in total soluble protein from δGMDδXylT plants increased by 82.41%. Recombinant mouse granulocyte/macrophage-colony stimulating factor (mGM-CSF) and human monoclonal immunoglobulin G (hIgG) harboring N-glycans with deleted plant-specific sugar residues were successfully produced in δGMDδXylT plants. Simultaneous repression of the GMD and XylT genes in N.benthamiana is thus very useful for deleting plant-specific sugar residues. © 2014 The Society for Biotechnology, Japan.

Ito A.,Japan National Institute of Advanced Industrial Science and Technology | Gotanda T.,The Kitasato Institute | Himeno N.,The Kitasato Institute | Itchoda N.,Japan National Institute of Advanced Industrial Science and Technology | And 4 more authors.
Avian Pathology | Year: 2013

We developed a transgenic potato (TrP/R7) expressing the recombinant R7 (rR7) antigen for use as an oral vaccine to protect against a chicken protozoan disease, chicken leucocytozoonosis. The TrP/R7 potato was produced by Agrobacterium tumefaciens-mediated transformation and regeneration, and the R7 gene insertion into potato chromosomes was confirmed by genomic polymerase chain reaction and Southern hybridization. rR7 antigen expression in TrP/R7 potato was also confirmed by sandwich enzyme-linked immunosorbent assay and western blotting using an antibody against the second-generation schizont of Leucocytozoon caulleryi. A transgenic potato clone with the highest rR7 antigen expression (3 μg rR7 antigen per gram of fresh-weight potato leaves) was selected, cultivated, and used in oral administration experiments to examine its ability to boost immunity. Chickens were immunized with chicken leucocytozoonosis vaccine "Hokken" by injection, and chickens that developed moderate levels of antibody titres were fed with TrP/R7 leaves. Chickens fed with TrP/R7 leaves showed increased antibody responses. In contrast, chickens fed with non-transgenic potato leaves showed a continuous decrease in antibody titres. Furthermore, chickens fed with TrP/R7 potato leaves showed strong resistance against experimental challenge with L. caulleryi infection. This study demonstrates the use of a plant-based oral vaccine to boost immunity against a protozoan disease. © 2013 Copyright Houghton Trust Ltd.

Hokusan Co., Japan National Institute of Advanced Industrial Science and Technology | Date: 2015-02-24

The present invention provides oral compositions which contain interferon (IFN) as an active ingredient for preventing and/or treating periodontal disease. The number of causative microorganisms of periodontal disease can be suppressed by administering the compositions into the oral cavity. IFN of the present invention can produce a sufficient effect even when administered at a very low dose. Furthermore, the compositions of the present invention can also be readily administered to animals such as dogs by formulating them into feed or such.

Japan National Institute of Advanced Industrial Science, Technology, Hokkaido University and Hokusan Co. | Date: 2010-01-18

Disclosed is a technique that facilitates the oral ingestion of adiponectin in a large quantity and enables the expansion of the range of use applications of adiponectin. Specifically disclosed is an appetite control agent for oral administration, which comprises adiponectin as an active ingredient. Particularly, the appetite control agent comprises a transformant capable of expressing adiponectin. Also specifically disclosed is a food composition for controlling appetite, which comprises the appetite control agent.

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