Hokkaido Research Organization HRO Central Agricultural Experiment Station AES

Naganuma, Japan

Hokkaido Research Organization HRO Central Agricultural Experiment Station AES

Naganuma, Japan
SEARCH FILTERS
Time filter
Source Type

Yamashita Y.,Hokkaido Research Organization HRO Central Agricultural Experiment Station AES | Takeuchi T.,Hokkaido Research Organization HRO Central Agricultural Experiment Station AES | Okuyama M.,HRO Kitami AES | Sasaki J.,HRO Kitami AES | And 4 more authors.
Breeding Science | Year: 2015

The yellowing strain of Soybean dwarf virus (SbDV-YS) causes yellowing and yield loss in common bean (Phaseolus vulgaris). The most effective control is achieved through breeding for resistance. An indeterminate climbing cultivar with a white seed coat, ‘Oofuku’, is resistant to SbDV-YS in inoculation tests. We crossed ‘Oofuku’ with an elite cultivar, ‘Taisho-Kintoki’, which is SbDV-YS-susceptible, determinate dwarf with a red-purple seed coat, and performed amplified-fragment-length polymorphism analysis of F3 lines. From nucleotide sequences of the resistant-specific fragments and their flanking regions, we developed five DNA markers, of which DV86, DV386, and DV398 were closely linked to Sdvy-1, a resistance gene. Using the markers, we developed ‘Toiku-B79’ and ‘Toiku-B80’, the near-isogenic lines (NILs) incorporating Sdvy-1 in the background of ‘Taisho-Kintoki’. The NILs had similar growth habit, maturity date and seed shape to those of ‘Taisho-Kintoki’. The quality of boiled beans was also similar, except that the NILs had more seed coat cracking than ‘Taisho-Kintoki’. The NILs showed no SbDV-YS infection in inoculation tests. We suggest that Sdvy-1 is a useful source of SbDV-YS resistance in common bean. © 2014, Japanese Society of Breeding. All rights reserved.


PubMed | HRO Tokachi AES, HRO Kitami AES and Hokkaido Research Organization HRO Central Agricultural Experiment Station AES
Type: Journal Article | Journal: Breeding science | Year: 2015

The yellowing strain of Soybean dwarf virus (SbDV-YS) causes yellowing and yield loss in common bean (Phaseolus vulgaris). The most effective control is achieved through breeding for resistance. An indeterminate climbing cultivar with a white seed coat, Oofuku, is resistant to SbDV-YS in inoculation tests. We crossed Oofuku with an elite cultivar, Taisho-Kintoki, which is SbDV-YS-susceptible, determinate dwarf with a red-purple seed coat, and performed amplified-fragment-length polymorphism analysis of F3 lines. From nucleotide sequences of the resistant-specific fragments and their flanking regions, we developed five DNA markers, of which DV86, DV386, and DV398 were closely linked to Sdvy-1, a resistance gene. Using the markers, we developed Toiku-B79 and Toiku-B80, the near-isogenic lines (NILs) incorporating Sdvy-1 in the background of Taisho-Kintoki. The NILs had similar growth habit, maturity date and seed shape to those of Taisho-Kintoki. The quality of boiled beans was also similar, except that the NILs had more seed coat cracking than Taisho-Kintoki. The NILs showed no SbDV-YS infection in inoculation tests. We suggest that Sdvy-1 is a useful source of SbDV-YS resistance in common bean.

Loading Hokkaido Research Organization HRO Central Agricultural Experiment Station AES collaborators
Loading Hokkaido Research Organization HRO Central Agricultural Experiment Station AES collaborators