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Yamashita Y.,Hokkaido Research Organization Central Agricultural Experiment Station | Takeuchi T.,Hokkaido Research Organization Central Agricultural Experiment Station | Ohnishi S.,Hokkaido Research Organization Kitami AES | Sasaki J.,Hokkaido Research Organization Kitami AES | Tazawa A.,Hokkaido Research Organization Tokachi AES
Breeding Science | Year: 2013

Soybean dwarf virus (SbDV), a Luteoviridae family member, causes dwarfing, yellowing and sterility of soybean (Glycine max), leading to one of the most serious problems in soybean production in northern Japan. Previous studies revealed that the Indonesian soybean cultivar 'Wilis' is resistant to SbDV and that the resistance can be introduced into Japanese cultivars. A major QTL for SbDV resistance has been reported between SSR markers Sat_217 and Satt211 on chromosome 5. In this study, we named this QTL Rsdv1 (resistance to SbDV) and developed near-isogenic lines incorporating Rsdv1 (Rsdv1-NILs) using Sat_217 and Satt211 markers. The Rsdv1-NILs were resistant to SbDV in greenhouse inoculation and field tests, indicating that Rsdv1 alone is sufficient for the resistance phenotype. We fine-mapped Rsdv1 within the 44-kb region between Sat_11 and Sct_13. None of the six genes predicted in this region was closely related to known virus resistance genes in plants. Thus, Rsdv1 may confer resistance by a previously unknown mechanism. We suggest that Rsdv1 may be a useful source for the Japanese soybean breeding program to introduce SbDV resistance. ©2013 by JAPANESE SOCIETY OF BREEDING.


Ohnishi S.,Hokkaido Research Organization Tokachi Agricultural Experiment Station | Ohnishi S.,Hokkaido Research Organization Central Agricultural Experiment Station | Funatsuki H.,Japan National Agricultural Research Center | Kasai A.,Hirosaki University | And 13 more authors.
Theoretical and Applied Genetics | Year: 2011

In yellow soybean, seed coat pigmentation is inhibited by post-transcriptional gene silencing (PTGS) of chalcone synthase (CHS) genes. A CHS cluster named GmIRCHS (Glycine max inverted-repeat CHS pseudogene) is suggested to cause PTGS in yellow-hilum cultivars. Cold-induced seed coat discoloration (CD), a commercially serious deterioration of seed appearance, is caused by an inhibition of this PTGS upon exposure to low temperatures. In the highly CD-tolerant cultivar Toyoharuka, the GmIRCHS structure differs from that of other cultivars. The aim of this study was to determine whether the variation of GmIRCHS structure among cultivars is related to variations in CD tolerance. Using two sets of recombinant inbred lines between Toyoharuka and CD-susceptible cultivars, we compared the GmIRCHS genotype and CD tolerance phenotype during low temperature treatment. The GmIRCHS genotype was related to the phenotype of CD tolerance. A QTL analysis around GmIRCHS showed that GmIRCHS itself or a region located very close to it was responsible for CD tolerance. The variation in GmIRCHS can serve as a useful DNA marker for marker-assisted selection for breeding CD tolerance. In addition, QTL analysis of the whole genome revealed a minor QTL that also affected CD tolerance. © 2010 Springer-Verlag.


Yamaguchi N.,Hokkaido Research Organization Tokachi Agricultural Experiment Station | Kurosaki H.,Hokkaido Research Organization Central Agricultural Experiment Station | Ishimoto M.,Japan National Institute of Agrobiological Science | Kawasaki M.,Hirosaki University | And 2 more authors.
Plant Production Science | Year: 2015

Early maturity is an important trait for soybean [Glycine max (L.) Merr.] growing in Hokkaido where the growing period is restricted because of the short fall season and early snowfall. Development of an early-maturing line without decreased seed yield is difficult because of the positive correlation between days to maturity and seed yield. In this study, we developed two breeding lines, Tokei 1067 (T1067) and Toiku 251 (T251), that were derived from crosses between Japanese and Polish cultivars. T1067 and T251 had a significantly earlier maturing time than Yukihomare (YH), the standard cultivar in Hokkaido. The seed yield of T251 was similar to that of YH. Moreover, the chilling tolerance levels of the T1067 and T251 lines at the flowering stage were greater than the tolerance level of YH. © 2015, Crop Science Society of Japan. All rights reserved.


PubMed | Hokkaido Research Organization Central Agricultural Experiment Station
Type: Journal Article | Journal: Breeding science | Year: 2012

Soybean dwarf virus (SbDV) causes serious dwarfing, yellowing and sterility in soybean (Glycine max). The soybean cv. Adams is tolerant to SbDV infection in the field and exhibits antibiosis to foxglove aphid (Aulacorthum solani), which transmits SbDV. This antibiosis (termed aphid resistance) is required for tolerance to SbDV in the field in segregated progenies of Adams. A major quantitative trait locus, Raso1, is reported for foxglove aphid resistance. Our objectives were to fine map Raso1 and to reveal whether Raso1 alone is sufficient to confer both aphid resistance and SbDV tolerance. We introduced Raso1 into cv. Toyomusume by backcrossing and investigated the degree of aphid antibiosis to foxglove aphid and the degree of tolerance to SbDV in the field. All Raso1-introduced backcross lines showed aphid resistance. Interestingly, only one Raso1-introduced backcross line (TM-1386) showed tolerance to SbDV in the field. The results demonstrated Raso1 alone is sufficient to confer aphid resistance but insufficient for SbDV tolerance. Tolerance to SbDV was indicated to require additional gene(s) to Raso1. Additionally, Raso1 was mapped to a 63-kb interval on chromosome 3 of the Williams 82 sequence assembly (Glyma1). This interval includes a nucleotide-binding site-leucine-rich repeat encoding gene and two other genes in the Williams 82 soybean genome sequence.


Tomita K.-I.,Hokkaido Research Organization Central Agricultural Experiment Station | Tomita K.-I.,Hokkaido Research Organization Kitami Agricultural Experiment Station | Hiura S.,Hokkaido Research Organization Central Agricultural Experiment Station | Tamagake H.,Hokkaido Research Organization Central Agricultural Experiment Station
Plant Biotechnology | Year: 2013

A wide range of genotypes suitable for a plant regeneration system is needed when utilizing genetic transformation techniques to develop new crop cultivars. We examined the regeneration efficiencies for somatic embryogenesis in 61 genetically diverse sugar beet (Beta vulgaris L.) breeding lines developed in Japan using a previously reported procedure. Frequencies of embryogenic callus formation from seedling leaf and petiole explants ranged from 0 to 89% and those of somatic embryo formation from the calli ranged from 0 to 99%. There was no clear correlation between the two frequencies, suggesting that different genetic backgrounds are involved in the two formation mechanisms. To evaluate each breeding line's capability for somatic embryogenesis, we proposed a somatic embryogenesis capability index based on four parameters. Based on the values of this index, only 21 breeding lines were suitable materials for regeneration via somatic embryogenesis. These breeding lines include four lines with moderate or better resistance to three major sugar beet diseases in Japan and one line with strong resistance to these diseases, and these lines will be useful materials with superior characteristics for developing transgenic breeding lines. To increase the regeneration efficiencies in the other 40 breeding lines, we examined other types of explants from seedlings and the effects of various plant growth regulators. We found that cotyledon explants generally showed improved callus formation and that using thidiazuron instead of N6-benzylaminopurine improved somatic embryo formation in some lines. © 2013 The Japanese Society for Plant Cell and Molecular Biology.

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