Köthen, Germany
Köthen, Germany

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The invention concerns a simple and effective process for preparing -carotene or lycopene by submerged fermentation with mixed cultures of (+) and () strains of the fungus Blakeslee trispora, characterized by high productivity of the B. trispora strains. The high productivity is achieved by virtue of the inventive regime, which acknowledges both the morphological state of the hemi-strains with their filamentous growth state when the strains are mixed (referred to as mating).

Agency: Cordis | Branch: FP7 | Program: CP-TP | Phase: KBBE.2012.1.2-03 | Award Amount: 7.69M | Year: 2012

BIOFECTOR is an integrated project that develops alternative fertilisation strategies by the use of various bio-effectors (BEs, plant growth promoting microorganisms and natural extraction products). BEs stimulate root growth, solubilise and mineralise sparingly available nutrients, or protect plants from abiotic and biotic stresses. Novel BEs will be isolated, characterized and applied in strategic combination with alternative fertilisation strategies that include organic and low-input farming, use of waste recycling fertilizers, and fertiliser-placement technologies. Bio-effectors addressed comprise fungal strains of Trichoderma, Penicillium and Sebacinales, as well as bacterial strains of Bacillus and Pseudomonades with well-characterized root growth promoting and nutrient solubilising potential. Natural extraction products of seaweed, compost and plant extracts, as well as their purified active compounds are also tested in various combinations. Maize, wheat and tomato are chosen as representative crops. Laboratory and European-wide field experiments assure product adaptation to divers geo-climatic conditions. Viable alternatives to the conventional practice of mineral fertilisation are developed, towards environmental friendly agricultural practice with reduced agrochemical input.

Due to political and economic changes in East Germany in 1989 the animal population decreased drastically. The number of breeding sows decreased from 870.000 in 1989 to 459.00 in 2010. The time factor and animal welfare became key issues for the pig production. Following this individual farm systems were developed and the weaning, insemination and farrowing dates for the group farrowing system have been controlled. The insemination rate increased to 95%. The increased usage of production and zoo technical stimulation measures leaded to decrease of reproduction biologicals. Especially the heat synchronization of the gilts and partial induction of parturition was emphasized. Selective breeding measures next to improvement in the herd health management led to partial changes in the oestrus cycle of the sows. Shorter weaning to insemination intervals, a 3 week lactation period combined with an unexpected elongation of the gestation in high performance sows provoked a change of the production concept according to the "all in all out" principle.

Hausdorf L.,Leibniz Institute fur Agrartechnik Potsdam Bornim e.V. | Mundt K.,Leibniz Institute fur Agrartechnik Potsdam Bornim e.V. | Winzer M.,Hochschule Anhalt | Cordes C.,Hochschule Anhalt | And 3 more authors.
Food Microbiology | Year: 2013

A better and regular control of the production chain of fresh fruits and vegetables is necessary, because a contamination of the product by human- and phyto-pathogenic microorganisms may result in high losses during storage and poses a threat to human health. Therefore, detailed knowledge about the occurrence and the diversity of microorganisms within single processing steps is required to allow target-oriented produce safety control. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successfully used to identify bacterial colonies. Bacteria can be identified with high accuracy by comparing them with generated spectra of a reference database.In this study, spinach and wash water samples were taken of the complete process line of a spinach-washing plant. Bacteria in the samples were grown on plate-count, Arcobacter selective, marine and blood agar. In total, 451 colonies were evaluated by MALDI-TOF MS, 16S rRNA gene sequence and phylogenetic analysis.50% of the detected species belonged to the class of Gammaproteobacteria. Firmicutes were present with 22%. Mostly, the detected species showed 16S rRNA gene sequence dissimilarities larger than 1% to known reference species and, hence, could not be assigned to a distinct species. However, many isolated species belonged to genera which contain pathogenic or opportunistic pathogenic bacteria. In addition, the bacterial diversity on the spinach surface increased after the first washing step indicating a process-borne contamination of the spinach. © 2012 Elsevier Ltd.

Hartmann T.,Ahornstrasse 6 | Schnackel W.,Hochschule Anhalt | Krockel L.,Max Rubner Institute
Fleischwirtschaft | Year: 2013

Prepackaged deli-meats such as sliced emulsion-type sausages are microbiologically highly sensitive foods on which, during prolonged cold storage, besides lactic acid bacteria (LAB) also pathogenic listeria are able to multiply. Suitable LAB can make an important contribution to control listeria in these products, especially when chemical preservatives are omitted. The present study provides data on the growth of Listeria innocua - as a non-pathogenic surrogate for Listeria monocytogenes - and different LAB on sausages produced with and without nitrite (Bologna-type sausage and 'Gelbwurst') during packaged storage al 5 °C under modified atmosphere (100 % N2, N2/CO2 = 70/30). 'Gelbwursf under N2 without protective culture provided the most favourable growth environment for listeria while the opposite was true for Bologna-type sausage under N 2/CO2 with protective culture. LAB were not inhibited by 30 % CO2 and, a relatively weak effect was seen with listeria on 'Gelbwursf. A better control of listerial growth was seen with bacteriocinogenic cultures as compared to cultures devoid of bacteriocin production. The sakacin A producer Lactobacillus (Lb.) sakei Lb706 displayed the strongest antilisterial effect and, also the most favourable sensory characteristics. On 'Gelbwursf under N2, however, strain Lb706 provided sufficient protection only when inoculated at > 106 cfu/g. Of several LAB tested for their sensory effects on both sausage types only, strains of Lb. sakei performed best.

Electrodeionization is a combination of ion exchange with electrodialysis. The method is applicable both for ultrapure water production and environmentally-oriented purposes. However, application in ultrapure water preparation is dominating in practice up to now. The paper reviews the development of electrodeionization and presents the state of art. Basis constructions are shown and main problems are discussed. Occurrence of large differences in resin and electrolyte conductivity is one of the most decisive problems because it hinders continuous electrodeionization operation mode. The way out of the dilemma is the consecutive resin loading and its electrochemical regeneration. A calculation explains the method.

Bergmann M.E.H.,Hochschule Anhalt | Koparal A.S.,Anadolu University
Journal of Hazardous Materials | Year: 2011

Regeneration of spent accumulator acid could be an alternative process for crystallization, neutralisation and disposal. Therefore, for the first time in a study of the possibilities of electrochemical removal of antimony and accumulator acid regeneration on a laboratory scale, two synthetic and several real systems containing sulfuric acid of concentrations ranging between 28% and 36%, and antimony species were tested. Discontinuous electrochemical reactors with anion exchange membranes were successfully used in these experiments, which were conducted at a temperature of 35°C. Removal of antimony using cells that were not divided by a separator, however, was not possible. In selected experiments, by varying the electrode material, type of electrolyte, and cell current, the concentration of antimony could be reduced from the range of 5ppm to 0.15ppm. This resulted in current efficiencies between 0.00002% and 0.001%, and in specific electroenergy demands between 100WhL -1 and 2000WhL -1. In other experiments on substances with antimony contents up to 3500mgL -1, the current efficiencies obtained were more than a thousandfold higher. In contrast to the formally high relative energy consumption parameters absolute demand parameters are relatively small and favour the electrochemical method in small scale application. Besides plate electrodes, 3D-cathodes were used. Copper- and graphite cathodes produced the best results. © 2011 Elsevier B.V.

Due to their high stability and outstanding oxidation power doped diamond materials belong to the most fascinating electrodes in applied electrochemistry. During the last years they have aroused interest in the field of disinfection. As typical for chemical and electrochemical disinfection processes, benefit of disinfection has to be opposed to disadvantages of byproduct formation. Predictions with respect to reaction results are difficult. Essential influence factors are the composition of treated water, electrode potential and hydrodynamic conditions near the electrode surface. In waters of low ionic strength containing chloride ions an enlarged risk for chlorate and Perchlorate formation can be observed. The paper presents own results and summarizes state of development in the field. Formulation of new test and certification routines is recommended.

Schmid F.,University of Hamburg | Goepfert M.S.,University of Hamburg | Kuhnt D.,Hochschule Anhalt | Eichhorn V.,University of Hamburg | And 4 more authors.
Anesthesia and Analgesia | Year: 2011

BACKGROUND: Vital sign monitors and ventilator/anesthesia workstations are equipped with multiple alarms to improve patient safety. A high number of false alarms can lead to a "crying wolf" phenomenon with consecutively ignored critical situations. Systematic data on alarm patterns and density in the perioperative phase are missing. Our objective of this study was to characterize the patterns of alarming of a commercially available patient monitor and a ventilator/anesthesia workstation during elective cardiac surgery. METHODS: We performed a prospective, observational study in 25 consecutive elective cardiac surgery patients. In all patients, identically fixed alarm settings were used. All incoming patient data and all alarms from the patient monitor and the anesthetic workstation were digitally recorded. Additionally, the anesthesia workplace was videotaped from 2 different angles to allow retrospective annotation and correlation of alarms with the clinical situation and assessment of the anesthesiologists' reaction to the alarms. RESULTS: Of the 8975 alarms, 7556 were hemodynamic alarms and 1419 were ventilatory alarms. For each procedure, 359 ± 158 alarms were recorded, representing a mean density of alarms of 1.2/minute. CONCLUSION: Approximately 80% of the total 8975 alarms had no therapeutic consequences. Implementation of procedure-specific settings and optimization in artifact and technical alarm detection could improve patient surveillance and safety. © 2010 International Anesthesia Research Society.

The disclosure describes a method for performing a bandwidth test for communications from a first network station (20) to a second network station (21) of a communication network. Such bandwidth test is based on the so-called PRM model (in words probe rate model) in which iteratively a train of probe sample packets is transmitted from first station (20) to the second station (21) with a constant packet rate per iteration. The packet rate however is adapted to the available bandwidth on the data pipe per iteration. According to the disclosure the number of probe sample packets per train of probe sample packets is varied with the available bandwidth per iteration. This increases the accuracy of the test in particular in the range where a high data rate is available on the data pipe. Also a step decreasing factor (SDF) is applied with which the performance test is improved. Such step decreasing factor is dependent on the network jitter and further improves the performance of the test.

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