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Todorova K.,HLA Tissue Typing Laboratory | Zuber K.,HLA Tissue Typing Laboratory | Neuwirt P.,HLA Tissue Typing Laboratory | Arnold R.,Oncology and Tumorimmunology | And 3 more authors.
Tissue Antigens | Year: 2014

We report the new HLA-A*11:192 allele differing from A*11:01 by one nucleotide in exon 2. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Lachmann N.,Charite - Medical University of Berlin | Lachmann N.,HLA Tissue Typing Laboratory | Todorova K.,Charite - Medical University of Berlin | Todorova K.,HLA Tissue Typing Laboratory | And 4 more authors.
Transfusion Medicine and Hemotherapy | Year: 2013

The detection of antibodies against the human leukocyte antigen (HLA) complex has become indispensable in every clinical practice. The development of solid-phase assays like the Luminex allows the standardized measurement of anti-HLA antibodies (HLAab) with high sensitivity, albeit the relevance for some clinical settings remains a matter of debate. In this review we aim to describe the principle of Luminex-based antibody detection, including two modifications that allow identifying solely complement-activating antibodies. We then describe three applications for Luminex: i) detection of HLAab preceding solid-organ transplantation and monitoring of donor-specific antibodies posttransplant as a risk factor for antibody-mediated rejection; ii) presence of HLAab in recipients as a risk for graft failure in hematopoietic stem cell transplantation, especially in haploidentical or mismatched transplantations; iii) role of HLAab in blood transfusion including refractory thrombocytopenia and selection of suitable platelet donors, transfusion-related lung injury after plasma transfusion, and immunization against HLA after red blood cell transfusion despite leukodepletion. Although the Luminex platform constitutes a potent technology for HLA antibody detection, some drawbacks require the well-educated analysis and interpretation of data in critical cases. In addition, Luminex has become an important tool to identify clinically relevant antibodies. © 2013 S. Karger GmbH, Freiburg.

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