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Bhat R.,Gulbarga University | Ganachari S.,Gulbarga University | Deshpande R.,Hkes Matoshree Taradevi Rampure Institute Of Pharmaceutical Science | Ravindra G.,Indian Institute of Science | Venkataraman A.,Gulbarga University
Journal of Cluster Science | Year: 2013

In this research paper, we report on the rapid synthesis of silver nanoparticles using dried areca nut (Areca catechu). The microwave exposed aqueous areca nut powder when treated with the aqueous silver salt solution yielded irregular shaped silver nanoparticles. The formation and morphology of the nanoparticles are studied using UV-visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy and atomic force microscopy. The X-ray diffraction studies and energy dispersive X-ray analysis indicate that the particles are crystalline in nature. The understanding of capping of biological moiety is derived from Fourier transform infrared spectroscopy and the thermogravimetric analysis. The green chemistry approach for the synthesis of silver nanoparticles is modest, amenable for large scale commercial production. Further the biologically synthesized silver nanoparticles are known for their potential antibacterial activity. © 2012 Springer Science+Business Media New York. Source


Raghunandan D.,Hkes Matoshree Taradevi Rampure Institute Of Pharmaceutical Science | Ravishankar B.,Gulbarga University | Sharanbasava G.,Gulbarga University | Mahesh D.B.,Gulbarga University | And 3 more authors.
Cancer Nanotechnology | Year: 2011

Biofunctionalized gold and silver nanoparticles synthesized using different plant extracts of guava and clove in vitro anti-cancer efficacy against four different cancer cell lines human colorectal adenocarcinoma, human kidney, human chronic myelogenous, leukemia, bone marrow, and human cervix have been studied and reported. The present experimental study suggests that flavonoids functionalized gold nanoparticles synthesized using aqueous clove buds extract are more potential than guava leaf extract towards anti-cancer activities. The microscopic and 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) assay infer that the functionalized irregular shaped gold nanoparticles synthesized with aqueous clove bud extract showed a satisfactory anti-cancer effect on all the cell lines. The silver nanoparticles synthesized using same extracts are devoid of anti-cancer activity. The XTT assay revealed dose-dependent cytotoxicity to cancer cell lines. The study revealed that the free radicals generated by gold nanoparticles are responsible for anti-cancer effect. To confirm the free-radical scavenging efficacy of gold nanoparticle, nitric oxide assay is followed. We observed that the gold nanoparticles swabbed the free radicals in dose-dependent manner. With continued improvements, these nanoparticles may prove to be potential anti-cancer agents. © 2011 Springer-Verlag. Source


Ganachari S.V.,Gulbarga University | Bhat R.,Gulbarga University | Deshpande R.,Hkes Matoshree Taradevi Rampure Institute Of Pharmaceutical Science | Venkataraman A.,Gulbarga University
BioNanoScience | Year: 2012

In this present investigation, we report a simple, cost-effective, and eco-friendly method of synthesizing colloidal silver nanoparticles by using fungi Penicillium diversum. UV-visible spectroscopy studies were carried out to quantify the formation of silver nanoparticles. The X-ray diffraction pattern suggests the crystallinity of silver nanoparticles. Atomic force microscopy and transmission electron microscopy images show that the silver nanoparticles are polydispersed and are in a size range of 5 to 45 nm with an average size of 20 nm. From the Fourier transform infrared spectroscopy, we presume that the reductase enzyme present in the fungal extract may be responsible for the reduction and stabilization of the silver nanoparticles. The resultant silver nanoparticles showed effective antimicrobial activity against Escherichia coli, Salmonella typhi, Vibrio cholerae, and the clinical isolate of Paratyphia. © 2012 Springer Science+Business Media, LLC. Source


Bhat R.,Gulbarga University | Deshpande R.,Hkes Matoshree Taradevi Rampure Institute Of Pharmaceutical Science | Ganachari S.V.,Gulbarga University | Huh D.S.,Inje University | Venkataraman A.,Gulbarga University
Bioinorganic Chemistry and Applications | Year: 2011

This is a report on photo-irradiated extracellular synthesis of silver nanoparticles using the aqueous extract of edible oyster mushroom (Pleurotus florida) as a reducing agent. The appearance, size, and shape of the silver nanoparticles are understood by UV-visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The X-ray diffraction studies, energy dispersive X-ray analysis indicate that particles are crystalline in nature. Fourier transform infrared spectroscopy analysis revealed that the nanoparticles are covered with biomoieties on their surface. As can be seen from our studies, the biofunctionalized silver nanoparticles thus produced have shown admirable antimicrobial effects, and the synthetic procedure involved is eco-friendly and simple, and hence high range production of the same can be considered for using them in many pharmaceutical applications. Copyright © 2011 Ravishankar Bhat et al. Source


Bhat R.,Gulbarga University | Sharanabasava V.G.,Gulbarga University | Deshpande R.,Hkes Matoshree Taradevi Rampure Institute Of Pharmaceutical Science | Shetti U.,Government Arts and Science College | And 2 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2013

A green chemistry approach to the synthesis of gold nanoparticles using edible mushroom Pleurotus florida (Oyster mushroom) by photo-irradiation method has been attempted. The mixture containing the aqueous gold ions and the mushroom extract was exposed to sunlight; this resulted in the formation of biofunctionalized gold nanoparticles. These nanoparticles were characterized using various techniques like UV-visible spectroscopy; X-ray diffraction studies, Energy dispersive X-ray analysis, Field emission scanning electron microscopy, Atomic force microscopy, Transmission electron microscopy and Fourier transform infrared spectrometry. The obtained biofunctionalized gold nanoparticles showed effective anti-cancer property against four different cancer cell lines A-549 (Human lung carcinoma), K-562 (Human chronic myelogenous leukemia bone marrow), HeLa (Human cervix) and MDA-MB (Human adenocarcinoma mammary gland) and no lethal effect is observed in Vero (African green monkey kidney normal cell) cell lines. © 2013 Elsevier B.V. Source

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