Han Y.,Qingdao University |
Zhao Q.,Qingdao University |
Zhou J.,Qingdao University |
Shi R.,Qingdao Hiser Medical Center
American Journal of Cancer Research | Year: 2017
Colorectal cancer (CRC), presenting the third most common malignancy worldwide. In recent years, the aberrantly upregulation or downregulation of miRNAs in CRC have been evidenced in a number of studies. In this study, our results showed that the expression of miR-429 was significantly higher in CRC tissue compared with adjacent non-tumor tissue. In addition, our findings showed that miR-429 level was significantly associated with clinicoplathological features and prognosis of patients with CRC. Moreover, our findings showed that miR-429 exerted oncogenic effect by directly targeting HOXA5, a transcription factor of HOX families that is involved in the development and progression of CRC.
Simultaneous determination of seven ginsenosides in rat plasma by high-performance liquid chromatography coupled to time-of-flight mass spectrometry: Application to pharmacokinetics of Shenfu injection
Li Z.,Shandong University |
Li Z.,Qingdao Hiser Medical Center |
Zhang R.,Qingdao Hiser Medical Center |
Wang X.,Qingdao Hiser Medical Center |
And 3 more authors.
Biomedical Chromatography | Year: 2015
A high-performance liquid chromatography coupled to time-of-flight mass spectrometry (HPLC-TOF MS) method was successfully developed and validated for the identification and determination of seven ginsenosides, Re, Rf, Rb1, Rc, Rb2, Ro and Rd, in a Chinese herbal preparation, Shenfu injection, and rat plasma. Based on the method, the pharmacokinetic profiles of the seven ginsenosides were investigated following intravenous administration of single dose of Shenfu injection to six rats. The established method had high linearity, selectivity, sensitivity, accuracy and precision. The pharmacokinetic results showed that Rb1, Rc and Rb2 had similar pharmacokinetic profiles and relatively long half-life values (19.29±6.36, 29.54±22.91 and 35.60±30.66 h). The half-lives of Rf and Rd were 4.21±3.68 and 8.49±5.20 h, respectively, indicating that they could be metabolized more rapidly than Rb1, Rc and Rb2. © 2014 John Wiley & Sons, Ltd.
Jiang W.-J.,Qingdao Hiser Medical Center
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2012
Background: Increase in ophthalmic optical medical instruments and microsurgical applications leads to retinal photochemical damage and other problems delivery of a variety of devices, so the in-depth study and understanding of its pathogenesis after retina light damage can provide a reference for the clinical treatment of related diseases. Objective: This study was to investigate the therapeutic effect and relative mechanism of erythropoietin (EPO) on mouse retina photic injury by studying the expression of matrix metalloproteinases-2(MMP-2) and MMP-9. Methods: Fifty-two SPF BALB/c mice were randomized into normal control group, simple light-induced group and EPO pretreatment group by balloting method. The mice of simple light-induced group and EPO pretreatment group were continuously irradiated with 6000 lx diffuse light for 4 hours in a home-made box to establish the models of light-induced damage; while recombinant human EPO(rhEPO) of 5000 U/kg was intraperitoneally injected prior to the light exposure in the EPO pretreatment group. The expressions of MMP-2 and MMP-9 were examined at 6, 12, 36, 72, 96 hours and 7 days following light-exposure by immunohistochemistry. Results: Edema and structural disorder of RPE cells appeared in the simple light-induced group after light-exposure and aggravated with lapse of light-exposure time, but no similar change was seen until 7 days in the EPO pretreatment group. The immunohistochemistry findings showed that the expression of MMP-2(A value) in RPE cells was less in the normal mice. However, a large quantity of positive cells appeared in RPE layer 36 hours after light-exposure. Compared with the simple light-induced group, the positive expression of MMP-2 protein in EPO pretreatment group was significantly decreased, showing statistically significant differences among these three groups and different time points (Fgroup= 3.68, P = 0.04; Ftime = 9.13, P = 0.00). There was hardly any MMP-9 expression in the retina of the normal mice. In simple light-induced group, a few of positive cells appeared in RPE layer 6 hours after light-exposure and reached its peak 12 hours following light-exposure. The gradually down-regulation of MMP-9 expression happened 96 hours later following light-irradiation. The expression tendency of MMP-9 in EPO pretreatment group was similar to the simple light-induced group. Significant differences in expressions of MMP-9 were found among different groups and time points (Fgroup = 3.61, P = 0.04; Ftime= 16.91, P = 0.00). Conclusions: MMP-2 and MMP-9 may be involved in the mechanism of retina photic injury by down-regulating the expression of MMP-2 and MMP-9 in RPE cells. Copyright © 2012 by the Chinese Medical Association.
Hu K.-X.,Academy of Military Medicine science |
Wang M.-H.,Academy of Military Medicine science |
Fan C.,Qingdao Hiser Medical Center |
Wang L.,Academy of Military Medicine science |
And 2 more authors.
International Immunopharmacology | Year: 2011
The results of haploidentical hematopoietic stem cell transplantation (HSCT) have been disappointing due to the high incidence of severe graft-versus-host disease (GVHD) and infectious complications. It is well known that mesenchymal stem cells (MSCs) can prevent severe acute GVHD in HSCT. However, there is a controversy concerning whether MSC-mediated suppression of T cell functions is accompanied by inducing T cells maturation effects after HSCT. The CB6F1 (H-2bd) female mice irradiated with 8 Gy 60Co γ-rays were divided into two groups: mice in the MSCs group were infused with MSCs labeled with cm-DiI and mononuclear cells from the bone marrow and spleen of BALB/c (H-2d) mice; the control group was infused with only the mononuclear cells of BALB/c (H-2d) mice. After transplantation, chimerisms of donor MSCs were observed in the recipients. The recovery of the T-lymphocyte subpopulation, the proliferative activity of T-cells after stimulation with ConA, the mixed lymphocytes' reaction between donor and recipient and three parts, and the number of apoptosis thymus cells were compared in two groups. The results showed that MSCs preferentially homed to the thymus and grew there, a more rapid recovery of T-cells in the peripheral blood, and decreased the apoptosis of the thymocytes. Thus MSCs may affect the thymus in order to improve T-cells maturation and immune system recovery. © 2011 Elsevier B.V.
Li T.,Colorectal Center |
Gao F.,Qingdao Hiser Medical Center |
Zhang X.-P.,Colorectal Center
Oncology Reports | Year: 2015
MicroRNAs (miRNAs) are a conserved class of small non-coding RNAs that play important roles in diverse biological processes, including chemoresistance. However, the molecular mechanism as to how miR-203 modulates the chemosensitivity to 5-fluorouracil (5-FU) in colorectal cancer is poorly known. In the present study, we found that miR-203 was downregulated in the 5-FU-resistant cell line LoVo/5-Fu, and was inversely correlated with the extent of 5-FU chemoresistance. Cytotoxicity assay showed that the inhibition of miR-203 expression enhanced 5-FU chemoresistance in colorectal cancer cells, while miR-203 overexpression increased 5-FU chemosensitivity. We then validated that thymidylate synthase (TYMS) was a direct target of miR-203 and miR-203 suppressed TYMS protein levels. Silencing of TYMS enhanced 5-FU chemosensitivity, similar to the roles of miR-203. Finally, we discovered that miR-203 increased the inhibitory effects of 5-FU on tumor growth in vivo. Overall, our data indicate that miR-203 enhances 5-FU chemosensitivity via the downregulation of TYMS in colorectal cancer and provide important insight into the mechanism of 5-FU resistance in colorectal cancer patients. More important, the present study suggests that miR-203 has the potential as a therapeutic strategy for 5-FU-resistant colorectal cancer.
PubMed | Qingdao Hiser Medical Center and Shandong University
Type: Journal Article | Journal: Clinical and experimental pharmacology & physiology | Year: 2016
Icaritin, a hydrolytic product of icariin from the Epimedium genus, exerts anti-tumour effects on a variety of tumour cell types, mainly by inhibiting cell proliferation and inducing apoptosis. However, little is known about the role of icaritin in cancer invasion and epithelial-to-mesenchymal transition (EMT). In the present study, the glioblastoma (GBM) cell line U87MG was used as a model to investigate the effects of icaritin on the invasion and EMT of cancer cells. The results showed that icaritin significantly inhibited the invasion and EMT of GBM cells by targeting extracellular matrix metalloproteinase (EMMPRIN). Furthermore, the findings strongly indicate that the modulatory effect of icaritin on EMMPRIN is mediated via the PTEN/Akt/HIF-1 signalling pathway. The data provide the first experimental evidence of the inhibitory effect of icaritin on cancer cell invasion and EMT, thus highlighting the potential of icaritin to be employed as a promising anti-cancer agent in the treatment of GBM.
PubMed | Huangdao Traditional Chinese Medicine Hospital, Qingdao Hiser Medical Center and Shandong University
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been considered to be one of the most promising candidates in research on treatments for cancer, because it induces apoptosis in a wide variety of cancer cells but not in most normal human cell types. However, many cells including glioblastoma (GBM) cells are resistant to TRAIL-induced apoptosis, which limits the potential application of TRAIL in cancer therapy. Icaritin, a hydrolytic product of icariin from Epimedium Genus, has been identified as a potential therapeutic and preventive agent in renal cell carcinoma and breast cancer. In this study, we investigated whether Icaritin treatment could modulate TRAIL-induced apoptosis in GBM. The effect of icaritin on TRAIL sensitivity was assessed in human GBM U87 and U373 cells. The underlying regulatory cascades were approached by biochemical and pharmacological strategies. We found that nontoxic concentration of icaritin alone had no significant effect on the level of apoptosis, but a combination treatment of TRAIL and icaritin caused a significantly more profound apoptosis. The sensitization was accompanied by c-FLIP down-regulation and inhibition of NF-B activity. Studies have further demonstrated that silencing NF-B alone was sufficient to down-regulate c-FLIP expression and sensitized both tested cells to TRAIL-induced apoptosis. These data suggest that icaritin sensitizes TRAIL-induced tumor cell apoptosis via suppression of NF-B-dependent c-FLIP expression, providing in vitro evidence supporting the notion that icaritin is a potential sensitizer of TRAIL in anticancer therapy against human GBM.
PubMed | Ocean University of China and Qingdao Hiser Medical Center
Type: Journal Article | Journal: Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis | Year: 2016
Propylene glycol alginate sodium sulfate (PSS) is the worlds first oral heparinoid approved by Chinese Food and Drug Administration in 1987. Propylene glycol alginate sodium sulfate is produced by modifying partially hydrolyzed alginate, one of the most abundant marine polysaccharides isolated from brown algae, by epoxypropane esterification and by chemical sulfation. It is used for treating and preventing cardiovascular-related diseases. The low cost (US$1.29/100 tablets, 4 tablets/day), remarkable clinical effects, and convenient oral administration make PSS an ideal long-term prevention drug. Propylene glycol alginate sodium sulfate is available in most drug stores in China, and millions of patients take PSS routinely during the past 27 years. The 22 784 reported clinical cases as well as the structure, preparation, clinical efficacy, adverse reactions, pharmacokinetics, pharmacodynamics, and future perspectives of PSS based on the results of peer-reviewed publications will be discussed. This review should bring the knowledge of PSS gained in China to the world to stimulate in depth academic and clinical studies of PSS.
PubMed | Qingdao Hiser Medical Center
Type: Journal Article | Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics | Year: 2015
OBJECTIVE To assess the association of polymorphisms of TNF-alpha gene (rs1799724, rs1800630, rs1799964 and rs769178) and IL-13 gene (rs2158177 and rs1295687) with susceptibility to asthma among ethnic Chinese in Qingdao region. METHODS For 400 asthma patients and 200 healthy subjects, above polymorphisms were detected with a SNaPshot method. RESULTS For rs2158177, the frequency of genotype of GG in the asthma group was significantly lower than the control group (2.8% vs. 5%, OR = 0.31, 95%CI: 0.12-0.82, P = 0.021). No significant difference was detected in the genotypic frequencies for the remaining 5 polymorphisms between the two groups (All P > 0.05). CONCLUSION The study has indicated that rs2158177 polymorphism of the IL-13 gene is associated with asthma in ethnic Han Chinese from Qingdao. No association has been found between polymorphisms of TNF-alpha gene with susceptibility to asthma.
PubMed | b Bin Zhou Peoples Hospital, QingDao Hiser Medical Center and Xinxiang Central Hospital
Type: Journal Article | Journal: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis | Year: 2015
In the present work, we undertook the complete mitochondrial genome sequencing of an important Lung cancer model inbred rat strain for the first time. The total length of the mitogenome was 16,308bp. It harbored 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and one non-coding control region (D-loop region). The mutation events were also reported.