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Uenaka K.,Shiga University of Medical Science | Imai S.,Shiga University of Medical Science | Ando K.,Hino Memorial Hospital | Matsusue Y.,Shiga University of Medical Science
Journal of Orthopaedic Science | Year: 2010

Background: A scaffold-free cartilage construct, analogous to those found during embryonic precartilage condensation, has received much attention as a novel modality for tissue-engineered cartilage. In the present study, we developed an uncomplicated culture system by which scaffold-free cartilage-like tissues are produced using cell-cell interactions. With this system, we attempted to prevent dedifferentiation and reverse the phenotypic modulations by adjusting the cell density. We investigated whether low-intensity pulsed ultrasound (LIPUS) enhances matrix synthesis of the scaffold-free cartilage construct. Methods: Rat articular chondrocytes multiplied in monolayers were seeded onto the synthetic porous membrane at stepwise cell densities (i.e., 1.0, 2.0, and 4.0 × 107 cells/cm2) to allow formation of a scaffold-free cartilage construct via cell-cell interaction. The cartilage constructs were then stimulated by LIPUS for 20 min/day. To investigate the effect of LIPUS stimulation on matrix synthesis, expression of mRNA for cartilage matrix molecules was quantified by a real-time reverse transcription-polymerase chain reaction. Synthesis of type II collagen, type I collagen, and proteoglycan was also assessed histologically. Results: Only the chondrocytes cultured at high cell densities in the 2.0 × 10 7cells/cm2 group became concentrated and formed a plate-like construct similar to native articular cartilage by macroscopic and histological assessments. Statistical analysis on the matrix gene expression demonstrated that the levels of type II collagen and aggrecan mRNA of the 2.0 × 107cells/cm2 group were significantly higher than with the other two cell-density groups. Interestingly, the LIPUS application led to a statistically significant enhancement of aggrecan gene expression only in the 2.0 × 107 cells/cm2 group. Conclusions: The current study presents a semi-open static culture system that facilitates production of the scaffold-free constructs from monolayer-cultured chondrocytes. It suggests that the LIPUS application enhances matrix production in the construct, and its combination with the scaffold-free construct might become a feasible tool for production of implantable constructs of better quality. © 2010 The Japanese Orthopaedic Association. Source

Kario K.,Jichi Medical University | Ando S.-I.,Fukuokaken Saiseikai Futsukaichi Hospital | Kido H.,Meimai Central Hospital | Nariyama J.,Osaka General Hospital of West Japan Railway Company | And 7 more authors.
Journal of Clinical Hypertension | Year: 2013

The Ambulatory Blood Pressure Control and Home Blood Pressure (Morning and Evening) Lowering By N-Channel Blocker Cilnidipine (ACHIEVE-ONE) trial is a large-scale clinical study on blood pressure (BP) and pulse rate (PR) in the real world with use of cilnidipine, a unique L/N-type Ca channel blocker, possessing a suppressive action on increased sympathetic activity in patients with essential hypertension. The effects of cilnidipine on morning hypertension were examined. The authors examined 2319 patients treated with cilnidipine for 12weeks. Clinic systolic BP (SBP) decreased by 19.6mm Hg from 155.0mm Hg, whereas morning SBP decreased by 17.0mm Hg from 152.9mm Hg after 12-week cilnidipine treatment. Cilnidipine reduced both morning SBP and PR more markedly in patients with higher baseline morning SBP (-3.2mm Hg and -1.3beats per minute in the first quartile of morning SBP, -30.9mm Hg and -3.2beats per minute in the fourth quartile), and also reduced both morning PR and SBP more markedly in patients with higher baseline morning PR (0.6beats per minute and -15.6mm Hg in <70beats per minute, and -9.7beats per minute and -20.2mm Hg in ≥85beats per minute). Cilnidipine significantly reduced BP and PR in hypertensive patients at the clinic and at home, especially with higher BP and PR in the morning. © 2012 Wiley Periodicals, Inc. Source

Hasegawa H.,Hino Memorial Hospital | Naitoh H.,Hino Memorial Hospital | Tsuchihashi H.,Hino Memorial Hospital | Hanasawa K.,Hino Memorial Hospital | And 2 more authors.
Japanese Journal of Cancer and Chemotherapy | Year: 2010

We report a patient with advanced gastric cancer with rhabdomyolysis. A 29-year-old man consulted a local doctor complaining of dysphagia and heartburn. He developed rhabdomyolysis that was considered to be caused by administered proton pump inhibitor (PPI). The patient has remained without recurrence for 18 months after operation. Source

Hasegawa H.,Hino Memorial Hospital | Naitoh H.,Hino Memorial Hospital | Tsuchihashi H.,Hino Memorial Hospital | Okada K.,Hino Memorial Hospital | And 2 more authors.
Japanese Journal of Cancer and Chemotherapy | Year: 2010

A 57-year-old woman underwent total hysterectomy and bilateral salpingo-oophorectomy for bilateral ovarian tumors (T1b, N0, M0; Stage I b, serous cystoadenocarcinoma) in May 1994. She received 4 courses of CAP (cyclophosphamide 500 mg/m&bsup;2&esup;+epirubicin 50 mg/m&bsup;2&esup;+cisplatin 60 mg/m&bsup;2&esup;) therapy as adjuvant chemotherapy. An increase of the serum CA125 was detected in December 2006, and abdominal CT revealed a mass in the spleen. Since whole-body FDG-PET-CT showed no evidence of local recurrence of the ovarian cancer or multiple organ metastases, a solitary splenic metastasis from ovarian cancer, even though very rare, was strongly suspected. Splenectomy with distal pancreatectomy was undertaken in December 2007. Histopathological examination revealed poorly-differentiated adenocarcinoma, suggesting the final diagnosis of solitary splenic metastasis from ovarian cancer. Periodic CA125 measurements are considered very important even over 10 years after surgery for ovarian cancer. Once solitary splenic metastasis is diagnosed, surgical resection would be the treatment of choice. Source

Tsuchihashi H.,Hino Memorial Hospital | Ito A.,Hino Memorial Hospital | Tsukada H.,Hino Memorial Hospital | Hasegawa H.,Hino Memorial Hospital | And 3 more authors.
Japanese Journal of Cancer and Chemotherapy | Year: 2011

A 56-year-old female patient was admitted, complaining of hematochezia. She was preoperatively diagnosed with poorly-differentiated carcinoma of anorectum with metastases in the lung and mesorectal lymph nodes, and underwent abdominoperineal resection of the rectum. The immunohistochemistry of the rectal tumor showed positive for vimentin and c-kit, and negative for AE1/AE3, S-100, α-SMA, LCA and CD34, which was compatible with gastrointestinal stromal tumor (GIST). Regardless of the administration of imatinib mesylate, multiple metastases in the brain, bone, adrenal glands and inguinal lymph node proceeded in a short term. An excisional biopsy of the inguinal lymph node was performed and immunohistochemistry of the specimen showed positive for S-100, melan-A, HMB45 and tyrosinase. Therefore, we concluded that amelanotic anorectal melanoma (AMM) metastasized to the lymph node, and rechecked the immuno histochemistry of the anorectal tumor. The anorectal tumor showed positive for melan-A, HMB45 and tyrosinase, but negative for S-100. As far as we know, there are few reports of AMM with S-100 negative and c-kit positive. In such cases, making a differential diagnosis between AMM and GIST of the anorectal region can be very confusing. Source

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