Kamal R.,Himachal Institute of Dental science |
Journal of Natural Science, Biology and Medicine | Year: 2011
The nasolabial cyst is a rare nonodontogenic cyst originating in maxillofacial soft tissues. In the previous reports and literature, it is mentioned that nasolabial cyst clinical features of an asymptomatic swelling beneath the ala of nose and characteristic lip swelling are diagnostic. This is a case report of a 36-year-old male, who reported to our department with the chief complaint of swelling lateral to right ala of nose since 4 months. Characteristic lip swelling was missing. The reason for this unusual finding is not documented in the literature.
PubMed | Dental College and Research Center, Shree Sai Dental College and Research Institute, Shree Guru Gobind Singh Tricentenary University, Himachal Institute of Dental science and Modern Dental College and Research Center
Type: Journal Article | Journal: Journal of International Society of Preventive & Community Dentistry | Year: 2016
Acrylic teeth separates from the denture base and remains a major worry in day-to-day routine dental procedure. The present study was conducted to comparatively evaluate different mechanical modifications of acrylic teeth on bond strength between Lucitone 199 heat cure resin and cross-linked teeth.The test specimens, central incisors (21) were demarcated into four groups. Group 1 was the control group, whereas Group 2, Group 3, and Group 4 were experimental groups modified with round groove, vertical groove, and T-shaped groove, respectively. The preparation of masterpiece was done by aligning the long axis of the central incisor teeth at 45 to the base of a wax block (8 mm 10 mm 30 mm), with ridge lap surface contacting the base. These test specimen (21) was prepared by Lucitone 199 heat cure resin. Evaluation of bond strength of all the specimens was done using universal tester (materials testing machine). Shapiro-Wilk Test, one-way analysis of variance (ANOVA), and Bonferroni test were done to do statistical investigation.Group 1 specimens prepared by Lucitone 199 heat cure resin showed the lowest bond strength and Group 4 specimens prepared with T-shaped groove packed with Lucitone 199 exhibited the highest bond strength.The bond strength between Lucitone 199 heat cure resin and cross-linked teeth was increased when mechanical modifications was done on denture teeth. The specimens prepared with T-shaped groove packed with Lucitone 199 heat cure resin showed the highest bond strength followed by Group 3, Group 2, and lastly Group 1 prepared by Lucitone 199 heat cure resin.
Chauhan I.,Himachal Institute of Dental science |
Beena V.T.,Government Dental College |
Srinivas L.,Rajiv Gandhi Center for Biotechnology |
Sathyan S.,Rajiv Gandhi Center for Biotechnology |
Banerjee M.,Rajiv Gandhi Center for Biotechnology
Journal of Interferon and Cytokine Research | Year: 2013
Oral lichen planus (OLP) is a chronic mucocutaneous condition that affects the oral mucous membrane as well as skin. It is a chronic cell-mediated autoimmune condition where the T-cell-mediated immune response plays an important part in the pathogenesis by causing damage to basal keratinocytes in oral mucosa. Cytokine gene polymorphisms have an unquestionable role in the orchestration of the immune response, leading to different functional scenarios, which in turn influence the outcome of the disease establishment and evolution. The purpose of this study was to understand the role of these cytokine gene polymorphisms in the tumor necrosis factor-Alpha (TNF-α), interleukin-1β (IL-1β), and IL-6 genes with OLP in 101 individuals of Malayalam-speaking ethnicity from South India (Kerala). We further investigated the role of these polymorphisms in patients suffering from OLP with other comorbid factors. Genotyping was carried out by polymerase chain reaction-restriction fragment length polymorphism. The results demonstrate that the A allele in the TNF-α -308 polymorphism could play an important role in the susceptibility to OLP. IL-1β +3954 in OLP was associated with other comorbid factors in both allelic and genotypic combinations. However, when patients suffering from OLP were stratified to understand the involvement of other comorbid factors, we observed that the T and C alleles were independent risk factors for chronic periodontitits and diabetes mellitus. On the other hand, IL-6 -597 did not show any disease association with OLP in the study population. This study indicates that proinflammatory cytokines are an important factor in understanding the disease burden of OLP and their comorbid factors. © Copyright 2013, Mary Ann Liebert, Inc. 2013.
PubMed | Daswani Dental College and Research Center, Himachal Institute of Dental science, Institute of Dental science, BRS Dental College and General Hospital and Army Dental Corps
Type: Journal Article | Journal: Journal of forensic dental sciences | Year: 2016
The identification of living or deceased persons using unique traits and characteristics of the teeth and jaws is a cornerstone of forensic science. Teeth have been used to estimate age both in the young and old, as well as in the living and dead. Gradual structural changes in teeth throughout life are the basis for age estimation. Tooth cementum annulation (TCA) is a microscopic method for the determination of an individuals age based on the analysis of incremental lines of cementum.To compare ages estimated using incremental lines of cementum as visualized by bright field microscopy, polarized microscopy, and phase contrast microscopy with the actual age of subject and to determine accuracy and feasibility of the method used.Cementum annulations of 60 permanent teeth were analyzed after longitudinal ground sections were made in the mesiodistal plane. The incremental lines were counted manually using a light, polarized and phase contrast microscopy. Ages were estimated and then compared with the actual age of individual.Analysis of variance (ANOVA), Students t-test, the Pearson product-moment corre (PPMCC) and regression analysis were performed.PPMCC value r = 0.347, 0.542 and 0.989 were obtained using light, polarized and phase contrast microscopy methods respectively.It was concluded that incremental lines of cementum were most clearly visible under a phase contrast microscope, followed by a polarized microscope, and then a light microscope when used for age estimation.
Bhalla G.,Himachal Institute of Dental science |
Astekar M.S.,Institute of Dental science |
Ramesh G.,Rama Dental College and Hospital |
Kaur P.,Darshan Dental College and Hospital |
Sowmya G.V.,Institute of Dental science
Biotechnic and Histochemistry | Year: 2014
Collagenase-3 (matrix metalloproteinase-13) is a metalloproteinase (MMP) that is associated with bone lesions and exhibits variable expression patterns in odontogenic cysts; it may play a role in regulating focal proliferation and maturation of jaw cyst epithelium. We studied the localization, staining intensity and distribution of collagenase-3 in 13 periapical granulomas with epithelium, 16 periapical granulomas without epithelium and 10 radicular cysts using archived formalin fixed, paraffin embedded tissues. A monoclonal antibody against human collagenase-3 was used to evaluate its expression. Immunohistochemical staining intensities of collagenase-3 in all periapical lesions were (-), 4 (10%); (+), 1 (3%); (++), 22 (56%) and (+++), 12 (31%); differences were not statistically significant. Immunohistochemical distribution of collagenase-3 in epithelial cells was (-), 17 (44%); (+), 17 (44%); (++), 5 (13%); in fibroblasts it was (-), 8 (20%); (+), 23 (59%); (++), 8 (21%); in plasma cells it was (-), 7 (18%); (+), 22 (56%); (++), 10 (26%); in macrophages it was (-), 7 (18%); (+), and 15 (38%); and (++), 17 (44%). Statistically significant differences were found in epithelial cells (p = 0.00) and fibroblasts (p = 0.02), whereas differences were not statistically significant for plasma cells and macrophages. Collagenase-3 may play a role in the conversion of a periapical granuloma with epithelium to radicular cyst. MMP's influence not only epithelial rest cell migration, but also invasion of various stromal cells into granulomatous tissue. © 2014 The Biological Stain Commission.
Sharma R.D.,Himachal Institute of Dental science
Indian journal of dental research : official publication of Indian Society for Dental Research | Year: 2011
Recent advances in resin adhesives and restorative materials, as well as an increased demand for esthetics, have lead to the introduction of newer resin-based composites like nanocomposites and light-curing units like light emitting diodes (LEDs). The present study was conducted to evaluate the effect of conventional quartz tungsten halogen (QTH) curing unit and blue LED on marginal adaptation of microhybrid composite (Filtek Z250) and nanocomposite (Filtek Z350) resins. Eighty Class V cavities were prepared on, extracted human premolars and were divided into four groups of 20 each. The four groups were designed according to the restorative resin and light cure unit used microhybrid/QTH, nanocomposites/QTH, microhybrid/LED and nanocomposites/LED. After thermocycling and immersion in 2% Basic Fuschin dye solution, the teeth were sectioned and dye penetration was observed under a stereomicroscope at 20X magnification. Statistical Analysis: All the samples were scored and results were analyzed using the "Kruskal Wallis and Mann Whitney tests" with "Wilcoxone correction." The results revealed least microleakage in microhybrid composites exposed to QTH and maximum in nanocomposites exposed to LED. Conclusion: Microhybrid composites exhibited lesser microleakage than nanocomposite resins.
Batra R.,Himachal Institute of Dental science
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology | Year: 2012
Failures in the normal course of embryonic development of the neural tube may give rise to a great variety of defects as the head and neck region develops. Such abnormalities may be manifested in the form of choristomas. The term choristoma applies to a cohesive tumor like mass consisting of normal cells in an abnormal location. Because of their benign tumor like growth and also their developmental pathogenesis, these need to be differentiated from neoplasms. Choristomas should also be distinguished from hamartomas which are tumor like malformations composed of a focal overgrowth of mature normal cells located where they are normally found. Since choristomas may arise from various tissues, mimicking various neoplasms, this review highlights the pathogenesis of different types of choristomas in the oral cavity. © 2011 Asian Association of Oral and Maxillofacial Surgeons.
Kamal R.,HPGDC |
Dahiya P.,Himachal Institute of Dental science |
Puri A.,Himachal Institute of Dental science
Journal of Oral and Maxillofacial Pathology | Year: 2012
Pyogenic granuloma or granuloma pyogenicum is a well-known oral lesion. The name pyogenic granuloma is a misnomer since the condition is not associated with pus and does not represent a granuloma histologically. Pyogenic granuloma of the oral cavity is known to involve the gingiva commonly. Extragingivally, it can occur on the lips, tongue, buccal mucosa, palate, and the like. A history of trauma is common in such sites. The etiology of the lesion is not known, though it was originally believed to be a botryomycotic infection. It is theorized that pyogenic granuloma possibly originates as a response of tissues to minor trauma and/or chronic irritation, thus opening a pathway for invasion of nonspecific microorganisms, although microorganisms are seldom demonstrated within the lesion. Pathogenesis of pyogenic granuloma is still debatable. Medline and PubMed databases were searched under the following key terms: Pathogenesis of oral pyogenic granuloma, pyogenic granuloma, and oral pyogenic granuloma. This search was limited to articles on human/animal studies which were published in English language. After reviewing the searched articles, the relevant articles were selected for the present review. Through this article, we have tried to summarize and present all the concepts of pathogenesis related to this most common and most mysterious oral lesion.
Roy S.,Himachal Institute of Dental science |
Garg V.,Himachal Institute of Dental science
Journal of Oral and Maxillofacial Pathology | Year: 2013
Objective: Keratocystic odontogenic tumor (KCOT) has an aggressive clinical course and a high tendency of recurrence, while orthokeratinized odontogenic cyst (OOC) has different characteristics and does not show aggressive behaviour. Even the treatment of these two lesions varies considerably. A large number of epithelial molecules have been studied in order to differentiate odontogenic keratocyst (OKC) from OOC, but stromal factors have not been adequately studied. Recently, tumor stroma has evolved as a particular field of interest. In the present study, we aim to evaluate and compare the expression of stromal myofibroblasts (MFs) in these entities and correlate it to its aggressive behavior. The term 'keratocystic odontogenic tumor' has been introduced by WHO in 2005 for odontogenic keratocyst keeping in mind its aggressive behavior, however still many pathologists and clinicians use the term OKC synonymously. Materials and Methods: A total of 10 cases of KCOT and 10 cases of OOC were stained for alpha-smooth muscle actin (αSMA) for demonstration of stromal MFs. MF frequency was assessed as the number of αSMA-positive stromal cells in 10 high power fields, presented as the mean number of positive cells per field. Results: Counts showed that the mean number of positive cells in KCOT (20.6 ± 2.05) was significantly higher than that seen in OOC (10.4 ± 1.06) (P < 0.05). Conclusion: The different behaviors of these lesions are compatible with the finding of the present study. The increased number of stromal MFs in KCOT in comparison to OOC correlates with its aggressive behavior and increased tendency towards recurrence.
Verma G.,Himachal Institute of Dental science
Pain Research and Treatment | Year: 2013
Trigeminal neuralgia (TN) is a clinical condition characterized by paroxysmal attacks of severe and electric shock-like pain along the distribution of one or more branches of the trigeminal nerve. Various medicinal or surgical modalities have been employed in the past with variable success. Newer methods were tried in search of permanent cure or long-lasting pain relief. The purpose of this paper is to present the review of the literature regarding the use of botulinum toxin type-A (BTX-A) in the management of trigeminal neuralgia. © 2013 Gaurav Verma.