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Gao T.,Highmark Renewables Research LP | Li X.,Highmark Renewables Research LP
Bioresource Technology | Year: 2011

The possibility of using anaerobic digestate effluent (ADE) to replace freshwater and nutrients for bioethanol production was explored. The ethanol concentration yielded from ADE and post-centrifuged ADE supernatant was 79.60 ± 1.75. g/L and 78.33 ± 1.66. g/L, respectively, with a 24% dry mass (DM) of soft wheat. Ethanol production was enhanced in ADE by as much as 18% in comparison to the production in freshwater (66.61 ± 0.28. g/L, p<. 0.01). Without yeast nutrients, ADE fermentation yielded an ethanol concentration of 81.10 ± 2.87. g/L, which was significantly higher than that in freshwater fermentation (59.67 ± 1.79. g/L). Analysis showed that ADE contained rich nitrogen, proteins and minerals. After one-step distillation, the ethanol concentration attained was 700.05 ± 46.20. g/L in ADE as compared to 622.79 ± 32.22. g/L in freshwater (p<. 0.05). © 2010 Elsevier Ltd. Source


Gao T.,Highmark Renewables Research LP | Bowlby E.,Alberta Agriculture and Rural Development | Tong Y.,Highmark Renewables Research LP | Wu J.T.Y.,Alberta Agriculture and Rural Development | And 4 more authors.
Bioresource Technology | Year: 2012

The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5. h in experiment 1 and for 16. h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p<. 0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5. h-treatment and was not present by 16. h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses. © 2012 Elsevier Ltd. Source

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