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Pyeongchang, South Korea

Lee K.-G.,Kyung Hee University | Lee S.-G.,Kyung Hee University | Lee H.-H.,Kyung Hee University | Lee H.J.,Kyung Hee University | And 6 more authors.
Chemico-Biological Interactions | Year: 2015

In this study, we investigated the molecular mechanisms underlying the anti-inflammatory effects of α-chaconine in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and in LPS-induced septic mice. α-Chaconine inhibited the expressions of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) at the transcriptional level, and attenuated the transcriptional activity of activator protein-1 (AP-1) by reducing the translocation and phosphorylation of c-Jun. α-Chaconine also suppressed the phosphorylation of TGF-β-activated kinase-1 (TAK1), which lies upstream of mitogen-activated protein kinase kinase 7 (MKK7)/Jun N-terminal kinase (JNK) signaling. JNK knockdown using siRNA prevented the α-chaconine-mediated inhibition of pro-inflammatory mediators. In a sepsis model, pretreatment with α-chaconine reduced the LPS-induced lethality and the mRNA and production levels of pro-inflammatory mediators by inhibiting c-Jun activation. These results suggest that the anti-inflammatory effects of α-chaconine are associated with the suppression of AP-1, and support its possible therapeutic role for the treatment of sepsis. © 2015 Elsevier Ireland Ltd. All rights reserved. Source

Shin D.-H.,Chungbuk National University | Kamal A.H.M.,Chungbuk National University | Suzuki T.,Japan National Agriculture and Food Research Organization | Yun Y.-H.,Highland Agriculture Research Center | And 7 more authors.
Australian Journal of Crop Science | Year: 2010

Buckwheat sprouts are a vegetable to provide health benefit with their nutritionally important substances. Despite several reports on describing buckwheat as preventive medicine, little has been studied on the developmental physiology of buckwheat in protein level. Thus, we attempted for the first time to examine the proteomic profiles of leaf and stem from 7-day-old etiolated sprouts of common (Fagopyrum esculentum Moench.) and tatary buckwheat (Fagopyrum tataricum Gaertn.) seedlings under light and dark. By the gelbased proteomic approach, 166 unique proteins were identified and six proteins were commonly found from both buckwheat species in which 79 and 81 proteins were exclusively belonged to common and tatary buckwheat, respectively. The most abundant proteins were assigned to metabolism and cytoplasmic proteins were dominated by the classification of molecular function and intracellular localization. The sprouting leaf of 7-day-old etiolated common buckwheat seedlings showed light yellow under dark, suggesting the inhibition of light-dependent protochlorophyllide reductase leading to the reduction of chlorophyll biosynthesis. Light-inhibited storage protein, 13S globulin 3, was commonly found in the leaves of both buckwheat species under dark. In particular, the unknown pentatricopeptide repeat-containing proteins were identified in light-induced leaf of common and tatary buckwheat and also identified in dark-induced stem of tatary buckwheat. Thus, it suggests that the different and specific regulators are involved in the sterility and fertility from common and tatary buckwheat in a specific light-dependent manner. The reference proteomic map of buckwheat will give insight for understanding buckwheat physiology and application to buckwheat industry. Source

Lee S.-J.,Kyung Hee University | Shin J.-S.,Kyung Hee University | Choi H.-E.,Kyung Hee University | Lee K.-G.,Kyung Hee University | And 7 more authors.
Food and Chemical Toxicology | Year: 2014

In this study, the authors investigated the molecular mechanism underlying the antiinflammatory effects of the chloroform fraction of the peel of 'Jayoung' (CFPJ), a color-fleshed potato, on lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and in mice with dextran sulfate sodium (DSS)-induced colitis. CFPJ inhibited the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the transcription level, and attenuated the transcriptional activity of nuclear factor-κB (NF-κB) by reducing the translocation of NF-κB depending on degradation of inhibitory κB-α (IκB-α). Furthermore, CFPJ attenuated the phosphorylations of mitogen-activated protein kinase kinases3/6 (MKK3/6) and of p38. In colitis model, CFPJ significantly reduced the severity of colitis and the productions and protein levels of pro-inflammatory mediators in colonic tissue. These results suggest that the anti-inflammatory effects of CFPJ are associated with the suppression of NF-κB and p38 activation in macrophages, and support its possible therapeutic role for the treatment of colitis. © 2013 Elsevier Ltd. Source

Jin Y.I.,Highland Agriculture Research Center | Cho J.H.,Highland Agriculture Research Center | Chang D.C.,Highland Agriculture Research Center | Im J.S.,Highland Agriculture Research Center | And 5 more authors.
Journal of Plant Biotechnology | Year: 2014

In order to research changes of asparagine content according to stage after harvest to storage, it was analysed asparagine content of potato tuber and potato sprout. The results of the asparagine content in potato cultivars after harvest ranked in cv. Superior, cv. Gahwang, cv. Atlantic and cv. Goun in descending order. The content of asparagine in potato was approximately 1300 mg/100g (D.W.) The changes the asparagine content according to storage condition and the difference of asparagine between tuber and sprout can be summarized as follows. In the asparagine content of potato cultivars, Little variation in the content was observed while potato tubers were stored at 4°C. However, after 3 months of storage at 10°C, the content increased by 9.7%, indicating that the content increases as the storage temperature and period increases. The content of asparagine also increased during sprouting of potato tubers. In the sprout, the proximal region contained 21% higher content of asparagines than the distal region, which suggests that the sprout develops with the increasing of the content of asparagines. © Korean Society for Plant Biotechnology. Source

Kim J.I.,Highland Agriculture Research Center | Kwon M.,Highland Agriculture Research Center | Kim G.-H.,Korea University | Kim S.Y.,Dongbu Hannong Co. | Lee S.H.,Seoul National University
Journal of Asia-Pacific Entomology | Year: 2015

The Aphis gossypii imidacloprid-resistant (IR) strain was about 3800-fold resistant to imidacloprid. Synergistic bioassays revealed that metabolic factors were not likely involved in resistance. An isogenic susceptible (IGS) strain was generated and used as references for RNA-seq, qrtPCR and 2DE. There were no noticeable differences between IGS and IR strains in either the transcriptome or proteome profiles, confirming that imidacloprid resistance is likely due to the target site insensitivity. The IR strain was determined to possess a point mutation resulting in an R81T substitution only in the nAChR beta 1 subunit, which had been also reported to be responsible for the reduced sensitivity to imidacloprid in M. persicae. An nAChR beta 1 subunit transcript variant in the N-terminal region was found and an additional point mutation, L80S was also detected along with the R81T mutation in IR strain, suggesting its potential role in resistance. Taken together, the R81T mutation in the nAChR beta 1 subunit, perhaps with the L80S mutation as well, could be the major factor of imidaloprid resistance in the IR strain and can be employed as molecular markers for the detection of imidacloprid resistance in field populations of A. gossypii. •We find new case in imidacloprid resistance mechanism in insect.•Mutations confer the resistance phenotype without P450-mediated detoxification.•The L80S mutation along with the R81T mutation in nAChR can be the main player to develop imidacloprid resistance. © 2015 . Source

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