Higher Institute of Biotechnology

Monastir, Tunisia

Higher Institute of Biotechnology

Monastir, Tunisia
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Ines M.,Higher Institute of Biotechnology | Ines M.,University of Sfax | Dhouha G.,Higher Institute of Biotechnology | Dhouha G.,University of Sfax
Carbohydrate Research | Year: 2015

Glycolipids, consisting of a carbohydrate moiety linked to fatty acids, are microbial surface active compounds produced by various microorganisms. They are characterized by highly structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface respectively. Rhamnolipids, trehalolipids, mannosylerythritol-lipids and cellobiose lipids are among the most popular glycolipids. Moreover, their ability to form pores and destabilize biological membrane permits their use in biomedicine as antibacterial, antifungal and hemolytic agents. Their antiviral and antitumor effects enable their use in pharmaceutic as therapeutic agents. Also, glycolipids can inhibit the bioadhesion of pathogenic bacteria enabling their use as anti-adhesive agents and for disruption of biofilm formation and can be used in cosmetic industry. Moreover, they have great potential application in industry as detergents, wetting agents and for flotation. Furthermore, glycolipids can act at the surface and can modulate enzyme activity permitting the enhancement or the inhibition of the activity of certain enzymes. © 2015 Elsevier Ltd.


Zidi I.,Higher Institute of Biotechnology | Bouaziz A.,Higher Institute of Biotechnology | Mnif W.,Higher Institute of Biotechnology | Bartegi A.,Higher Institute of Biotechnology | And 2 more authors.
Scandinavian Journal of Immunology | Year: 2010

Golimumab is a new approved humanized antibody for the treatment of rheumatoid arthritis (RA). This antibody belonging to biologic agents is raised against the pro-inflammatory cytokine tumour necrosis factor-α playing an essential role in the initiation of RA. To date, Golimumab administration for patients with RA, as indicated by USA Food and Drug Administration, is subcutaneous combined with methotrexate (MTX). Here, we have reviewed current literature with a focus on characteristics of Golimumab and also have exposed the clinical trials either using MTX or not using MTX. We have also highlighted the incoming clinical trials on Golimumab and have proposed some indications for the future studies based on a setting of clinical data and post-marketing observational studies. These studies will advance rheumatologists' decisions in the beginning of RA therapeutic interventions to insure the best outcomes for patients with RA and to improve their quality of life. © 2010 Blackwell Publishing Ltd.


Mnif I.,Higher Institute of Biotechnology | Mnif I.,University of Monastir | Ghribi D.,Higher Institute of Biotechnology | Ghribi D.,University of Monastir
Crop Protection | Year: 2015

Biopesticides, key components of integrated pest management programs, are receiving practical attention as a means to reduce the amount of synthetic chemical products being used to control plant pests and diseases and to protect stored products. A large number of bacterial derived products have been released, several of which have already played dominant roles in the market. Bacterial pesticides are used to control pests, pathogens and weeds by a variety of mechanisms. Among them, they might act as competitors or inducers of host resistance in plant. Some act by inhibiting growth, feeding, development or reproduction of a pest or pathogen. The aim of this review is to provide an overview of the use of bacterial derived biopesticides for pest management and to discuss the current development and application of their various types. Detailed classification of Bacillus thuringiensis, Bacillus subtilis and Bacillus sphaericus based biopesticide is provided along with their insecticidal, mosquitocidal, nematicidal and antimicrobial activities. The review revealed great potential for further exploitation of bacterial derived biopesticides in plant protection. Pseudomonas sp. derived biopesticides and their potential use as mosquitocide, nematicide, antimicrobial agents and inducer of systemic resistance in plants are also discussed. © 2015 Elsevier Ltd.


Ines M.,Higher Institute of Biotechnology | Ines M.,University of Monastir | Dhouha G.,Higher Institute of Biotechnology | Dhouha G.,University of Monastir
Peptides | Year: 2015

Abstract Lipopeptides are microbial surface active compounds produced by a wide variety of bacteria, fungi and yeast. They are characterized by highly structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface, respectively. Surfactin, iturin and fengycin of Bacillus subtilis are among the most studied lipopeptides. This review will present the main factors encountering lipopeptides production along with the techniques developed for their extraction and purification. Moreover, we will discuss their ability to form pores and destabilize biological membrane permitting their use as antimicrobial, hemolytic and antitumor agents. These open great potential applications in biomediacal, pharmaceutic and agriculture fields. © 2015 Elsevier Inc.


Zidi I.,Higher Institute of Biotechnology | Bouaziz A.,Higher Institute of Biotechnology | Mnif W.,Higher Institute of Biotechnology | Bartegi A.,Higher Institute of Biotechnology | Ben Amor N.,Higher Institute of Biotechnology
Medical Oncology | Year: 2011

Malignancy is one of the comorbidities linked to golimumab, a biological TNF-α blocker. In this systematic review and meta-analysis, we searched different databases and analyzed original publications to elucidate the remaining open question about the real association of malignancies with golimumab therapy. The most frequent cancer in patients treated with golimumab, in association or not with methotrexate, is the lung adenocarcinoma. However, lymphoma is not very commonly represented in these patients. We show that there is no major and evident risk of malignancies associated with golimumab in current scientific literature. An increased risk of malignancies may be associated with golimumab, but this warrants further clinical confirmation. Also, this risk mentioned in different studies must be taken with caution because of number of limits and biases. © 2010 Springer Science+Business Media, LLC.


PubMed | Higher Institute of Biotechnology
Type: | Journal: Carbohydrate research | Year: 2015

Glycolipids, consisting of a carbohydrate moiety linked to fatty acids, are microbial surface active compounds produced by various microorganisms. They are characterized by highly structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface respectively. Rhamnolipids, trehalolipids, mannosylerythritol-lipids and cellobiose lipids are among the most popular glycolipids. Moreover, their ability to form pores and destabilize biological membrane permits their use in biomedicine as antibacterial, antifungal and hemolytic agents. Their antiviral and antitumor effects enable their use in pharmaceutic as therapeutic agents. Also, glycolipids can inhibit the bioadhesion of pathogenic bacteria enabling their use as anti-adhesive agents and for disruption of biofilm formation and can be used in cosmetic industry. Moreover, they have great potential application in industry as detergents, wetting agents and for flotation. Furthermore, glycolipids can act at the surface and can modulate enzyme activity permitting the enhancement or the inhibition of the activity of certain enzymes.


PubMed | Higher Institute of Biotechnology
Type: Journal Article | Journal: Journal of dairy science | Year: 2015

This study was carried out to explore the adaptive mechanisms of Salmonella enterica serovar Typhimurium, in particular the implication of fatty acids (FA) in the remodeling of membrane lipid composition to overcome the combined effects of long-term starvation and -irradiation stresses. In addition, cell surface hydrophobicity was also evaluated. The bacterial strains (control and starved) were treated with a nonlethal -irradiation dose of 0.5 kGy and sublethal doses of 1 kGy. Gas chromatography analysis showed that the FA composition of starved and -irradiated cells was modified. However starvation combined with -irradiation induced more modifications in the FA composition than -irradiation or starvation alone. Indeed, the unsaturated FA-to-saturated FA ratio decreased significantly for both strains compared with -irradiated cells, as main consequence of the cyclic FA formation. Our results showed that starvation, irradiation, or combined stresses significantly influenced the hydrophobicity, and this may have affected the virulence state of Salmonella Typhimurium cells. This study represents one of the few to demonstrate the modifications on bacterial membrane as a cellular response to survive to the ionizing radiation combined with long-term starvation stress.


PubMed | Higher Institute of Biotechnology
Type: Journal Article | Journal: Medical oncology (Northwood, London, England) | Year: 2011

Malignancy is one of the comorbidities linked to golimumab, a biological TNF- blocker. In this systematic review and meta-analysis, we searched different databases and analyzed original publications to elucidate the remaining open question about the real association of malignancies with golimumab therapy. The most frequent cancer in patients treated with golimumab, in association or not with methotrexate, is the lung adenocarcinoma. However, lymphoma is not very commonly represented in these patients. We show that there is no major and evident risk of malignancies associated with golimumab in current scientific literature. An increased risk of malignancies may be associated with golimumab, but this warrants further clinical confirmation. Also, this risk mentioned in different studies must be taken with caution because of number of limits and biases.


PubMed | Higher Institute of Biotechnology
Type: Journal Article | Journal: Scandinavian journal of immunology | Year: 2010

Golimumab is a new approved humanized antibody for the treatment of rheumatoid arthritis (RA). This antibody belonging to biologic agents is raised against the pro-inflammatory cytokine tumour necrosis factor-alpha playing an essential role in the initiation of RA. To date, Golimumab administration for patients with RA, as indicated by USA Food and Drug Administration, is subcutaneous combined with methotrexate (MTX). Here, we have reviewed current literature with a focus on characteristics of Golimumab and also have exposed the clinical trials either using MTX or not using MTX. We have also highlighted the incoming clinical trials on Golimumab and have proposed some indications for the future studies based on a setting of clinical data and post-marketing observational studies. These studies will advance rheumatologists decisions in the beginning of RA therapeutic interventions to insure the best outcomes for patients with RA and to improve their quality of life.


Lagha R.,Higher Institute of Biotechnology | Ben Abdallah F.,Higher Institute of Biotechnology | Masmoudi A.S.,Higher Institute of Biotechnology
Journal of Dairy Science | Year: 2015

This study was carried out to explore the adaptive mechanisms of Salmonella enterica serovar Typhimurium, in particular the implication of fatty acids (FA) in the remodeling of membrane lipid composition to overcome the combination effects of long-term starvation and γ-irradiation stresses. In addition, cell surface hydrophobicity was also evaluated. The bacterial strains (control and starved) were treated with a nonlethal γ-radiation doses of 0.5 kGy and sublethal doses of 1 kGy. Gas chromatography analysis showed that the FA composition of starved and γ-irradiated cells was modified. However starvation combined with γ-irradiation induced more modifications in the FA composition than γ-irradiation or starvation alone. Indeed, the unsaturated FA-to-saturated FA ratio decreased significantly for both strains compared with γ-irradiated cells, as main consequence of the cyclic FA formation. Our results showed that starvation, irradiation, or combined stresses significantly influenced the hydrophobicity, and this may have affected the virulence state of Salmonella Typhimurium cells. This study represents one of the few to demonstrate the modifications on bacterial membrane as a cellular response to survive to the ionizing radiation combined with long-term starvation stress. © 2015 American Dairy Science Association.

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