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Bahri-Sahloul R.,Tunis el Manar University | Ben Fredj R.,University of Sousse | Boughalleb N.,Higher Institute of Agronomy of Chott Mariem | Shriaa J.,Laboratory of Environment Microbiology | And 6 more authors.
Journal of Botany | Year: 2014

Objective. Plant cell culture is an innovative technology to produce a variety of substances. Numerous plants synthesize among their secondary metabolites phenolic compounds which possess antioxidant and antimicrobial effects. Hawthorn (Crataegus) is one of these plants which has long been used in folk medicine and is widely utilized in pharmaceutical preparations mainly in neuro- and cardiosedative actions. Methods and Results. The production of polyphenol by fifty-two-week-old Crataegus azarolus var. aronia calli was studied in relation to growth variation and antioxidant and antimicrobial capacity within a subcultured period. The DPPH and ABTS+ assays were used to characterize the antioxidant actions of the callus cultures. Antimicrobial activity was tested by using disc diffusion and dilution assays for the determination of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values of each active extract. High TEAC TEAC and antimicrobial activity was observed when maximal growth was reached. An optimum of total phenol, proanthocyanidins, flavonoid, (-)-epicatechin, procyanidin B2, chlorogenic acid, and hyperoside was produced during this period. Conclusion. Antioxidant and antimicrobial activities were strongly correlated with total phenols and total flavonoids. Crataegus azarolus var. aronia cells culture represents an important alternative source of natural antioxidants and antimicrobials. © 2014 Radhia Bahri-Sahloul et al. Source

Ines M.,Higher Institute of Biotechnology | Ines M.,University of Sfax | Dhouha G.,Higher Institute of Biotechnology | Dhouha G.,University of Sfax
Carbohydrate Research | Year: 2015

Glycolipids, consisting of a carbohydrate moiety linked to fatty acids, are microbial surface active compounds produced by various microorganisms. They are characterized by highly structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface respectively. Rhamnolipids, trehalolipids, mannosylerythritol-lipids and cellobiose lipids are among the most popular glycolipids. Moreover, their ability to form pores and destabilize biological membrane permits their use in biomedicine as antibacterial, antifungal and hemolytic agents. Their antiviral and antitumor effects enable their use in pharmaceutic as therapeutic agents. Also, glycolipids can inhibit the bioadhesion of pathogenic bacteria enabling their use as anti-adhesive agents and for disruption of biofilm formation and can be used in cosmetic industry. Moreover, they have great potential application in industry as detergents, wetting agents and for flotation. Furthermore, glycolipids can act at the surface and can modulate enzyme activity permitting the enhancement or the inhibition of the activity of certain enzymes. © 2015 Elsevier Ltd. Source

Mnif I.,Higher Institute of Biotechnology | Mnif I.,University of Monastir | Ghribi D.,Higher Institute of Biotechnology | Ghribi D.,University of Monastir
Crop Protection | Year: 2015

Biopesticides, key components of integrated pest management programs, are receiving practical attention as a means to reduce the amount of synthetic chemical products being used to control plant pests and diseases and to protect stored products. A large number of bacterial derived products have been released, several of which have already played dominant roles in the market. Bacterial pesticides are used to control pests, pathogens and weeds by a variety of mechanisms. Among them, they might act as competitors or inducers of host resistance in plant. Some act by inhibiting growth, feeding, development or reproduction of a pest or pathogen. The aim of this review is to provide an overview of the use of bacterial derived biopesticides for pest management and to discuss the current development and application of their various types. Detailed classification of Bacillus thuringiensis, Bacillus subtilis and Bacillus sphaericus based biopesticide is provided along with their insecticidal, mosquitocidal, nematicidal and antimicrobial activities. The review revealed great potential for further exploitation of bacterial derived biopesticides in plant protection. Pseudomonas sp. derived biopesticides and their potential use as mosquitocide, nematicide, antimicrobial agents and inducer of systemic resistance in plants are also discussed. © 2015 Elsevier Ltd. Source

Ines M.,Higher Institute of Biotechnology | Ines M.,University of Monastir | Dhouha G.,Higher Institute of Biotechnology | Dhouha G.,University of Monastir
Peptides | Year: 2015

Abstract Lipopeptides are microbial surface active compounds produced by a wide variety of bacteria, fungi and yeast. They are characterized by highly structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface, respectively. Surfactin, iturin and fengycin of Bacillus subtilis are among the most studied lipopeptides. This review will present the main factors encountering lipopeptides production along with the techniques developed for their extraction and purification. Moreover, we will discuss their ability to form pores and destabilize biological membrane permitting their use as antimicrobial, hemolytic and antitumor agents. These open great potential applications in biomediacal, pharmaceutic and agriculture fields. © 2015 Elsevier Inc. Source

Zidi I.,Higher Institute of Biotechnology | Bouaziz A.,Higher Institute of Biotechnology | Mnif W.,Higher Institute of Biotechnology | Bartegi A.,Higher Institute of Biotechnology | And 2 more authors.
Scandinavian Journal of Immunology | Year: 2010

Golimumab is a new approved humanized antibody for the treatment of rheumatoid arthritis (RA). This antibody belonging to biologic agents is raised against the pro-inflammatory cytokine tumour necrosis factor-α playing an essential role in the initiation of RA. To date, Golimumab administration for patients with RA, as indicated by USA Food and Drug Administration, is subcutaneous combined with methotrexate (MTX). Here, we have reviewed current literature with a focus on characteristics of Golimumab and also have exposed the clinical trials either using MTX or not using MTX. We have also highlighted the incoming clinical trials on Golimumab and have proposed some indications for the future studies based on a setting of clinical data and post-marketing observational studies. These studies will advance rheumatologists' decisions in the beginning of RA therapeutic interventions to insure the best outcomes for patients with RA and to improve their quality of life. © 2010 Blackwell Publishing Ltd. Source

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