Higher Education Institutions in Fujian Province

Zhangzhou, China

Higher Education Institutions in Fujian Province

Zhangzhou, China

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Liu J.,Zhangzhou Normal University | Liu Z.-B.,Xiamen Third Hospital | Huang Q.,China Institute of Technology | Huang Q.,Higher Education Institutions in Fujian Province | And 7 more authors.
Analytical Methods | Year: 2014

A new solid substrate-room temperature phosphorimetry (SS-RTP) method for colchicine (COL) detection has been established based on its strong catalytic effect on H2O2oxidation of acridine yellow (AY), which sharply quenched the room temperature phosphorimetry (RTP) of AY. This highly sensitive (the limit of quantification (LOQ) was 3.1 × 10-13g mL-1), accurate and selective SS-RTP has been successfully applied for COL detection in human serum and tea samples with the results efficiently agreeing with the results for high performance liquid chromatography (HPLC); the results were also in agreement with those obtained by ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS). The activation energy and the reaction rate constant of catalytic reaction were 40.53 kJ mol-1and 3.97 × 10-4s-1, respectively. In addition, the reaction mechanism of catalytic SS-RTP for COL detection was also discussed using infrared spectra (IR), nuclear magnetic resonance (NMR) and electron impact mass spectra (EIMS). This journal is © the Partner Organisations 2014.


Liu J.-M.,Zhangzhou Normal University | Huang Q.,China Institute of Technology | Huang Q.,Higher Education Institutions in Fujian Province | Lin X.,Zhangzhou Normal University
Analytical Methods | Year: 2014

We report a new catalytic biosensor for the detection of rhamnose (Rha) based on combining the high sensitivity of a fluorescence method with the high selectivity of a catalytic reaction. The biosensor has the advantages of high sensitivity (limit of detection 1.9 × 10-17 g mL-1) and selectivity; it is non-radioactive, non-invasive and safe to use. The biosensor was used to determine trace amounts of Rha in cigarettes and jujubes and the results were in good agreement with those obtained by high-performance liquid chromatography. Calcein (R)-Rha was also determined by high-performance liquid chromatography, mass spectrometry and nuclear magnetic resonance. We also report the mechanism used in the catalytic biosensor for the determination of trace amounts of Rha. This journal is © the Partner Organisations 2014.

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