Higher Education Institute of Rab Rashid

Tabrīz, Iran

Higher Education Institute of Rab Rashid

Tabrīz, Iran

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Ebrahimian M.,Ferdowsi University of Mashhad | Ebrahimian M.,Mashhad University of Medical Sciences | Taghavi S.,Mashhad University of Medical Sciences | Mokhtarzadeh A.,Gonabad University of Medical Sciences | And 3 more authors.
Applied Biochemistry and Biotechnology | Year: 2017

In recent years, much effort has been focused on an appropriate combination of chemotherapeutic drugs and nucleic acids to exploit additive or synergistic therapeutic effects and overcome many obstacles such as the reduction of side effects and drug resistance. Short hairpin RNA (shRNA) has designed to allow the production of small interfering RNA (siRNA) within the cells and offer long-lasting silencing of target genes. In this study, alkyl-modified polyethylenimine (PEI 10 kD) was used for co-delivery of doxorubicin (DOX) encapsulated into poly lactic-co-glycolic acid (PLGA) nanoparticles (NPs) and Bcl-xL shRNA (one class of molecules that block apoptosis of tumor cells) into breast cancer cells. Our results demonstrated that modification of PEI with alkyl chain could enhance the induction of apoptosis in tumor cells by suppression of Bcl-xL gene using Bcl-xL shRNA more than PEI alone. On the other hand, DOX encapsulated into PLGA had more synergistic effect with shRNA in comparison with DOX alone. In conclusion, combination of PLGA-DOX NPs and alkyl-PEI/shRNA complexes may have promising applications in breast cancer therapy. © 2017 Springer Science+Business Media New York


Mokhtarzadeh A.,Tabriz University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | Alibakhshi A.,Shahid Beheshti University of Medical Sciences | Hejazi M.,Gonabad University of Medical Sciences | And 2 more authors.
TrAC - Trends in Analytical Chemistry | Year: 2016

There are several biocompatible and biodegradable biopolymers, which found tremendous medical and pharmaceutical applications and currently receiving unprecedented attention. Various microorganisms can synthesize a variety of these biopolymers such as polysaccharides, polyamides and polyesters, which could be used for development of new generation of drug carriers and tissue repairing materials. The size, charge, chemical structure and other physicochemical properties of bacterial biodegradable polymers represent a good compatibility in development of tissue scaffolds and also as delivery vehicles of therapeutic agents. Here, we highlight recent advances in engineering biopolymers derived from bacteria, especially for drug delivery and tissue engineering. © 2016 Elsevier B.V.


Mokhtarzadeh A.,Tabriz University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | Mokhtarzadeh A.,Mashhad University of Medical Sciences | Tabarzad M.,Shahid Beheshti University of Medical Sciences | And 4 more authors.
TrAC - Trends in Analytical Chemistry | Year: 2016

The development of enhanced drug delivery systems is one of the most attractive fields of pharmaceutical sciences, as some of the highly effective chemo/biotherapeutics for cancer treatment can not be administrated due to their high toxicities for normal cells or low stability in physiological media. However, drugs that are currently not administrable will become valuable if specific cell-targeted drug carriers can protect the normal cells from adverse effects and also improve drug pharmacokinetics. Aptamers are attractive and promising biomaterials developed with high affinity and specificity against numerous valuable targets. They could act similar to monoclonal antibodies (mAbs), and offer significant advantages. Combined with aptamers, nanostructures are smart veicles with remarkable properties for drug delivery. Combination of aptamer and nanotechnology has resulted in the production of various targeted drug delivery systems which are highly efficient in therapeutic and diagnostic applications. In this review, some of the efforts related to design and development of aptamer-targeted nanocarriers have been summarized considering: i) Aptamer importance as smart ligands and the aptamer development methods ii) Types of nanostructures combined with aptamers as targeting agent proposed in the literature iii) Cancer specific aptamers evaluated in combination with nanocarriers for diagnostic and therapeutic applications and iv) Discussion of aptamer-based smart nanocarriers according to the trend of related research works. © 2016 Elsevier B.V.


PubMed | Golestan University of Medical Sciences, Higher Education Institute of Rab Rashid and Tehran University of Medical Sciences
Type: Journal Article | Journal: Journal of medical virology | Year: 2016

Hepatitis B virus (HBV) infection is a major global health problem. Chronically infected people are at risk for progressive hepatic fibrosis and consequent cirrhosis. Hepatitis B surface antigen (HBsAg) level in serum is a complementary marker for intrahepatic HBV DNA and covalently closed circular DNA (cccDNA). Sortilin-1 (SORT1) has been reported to be involved in the post-Golgi vesicle trafficking of Apo lipoproteins degradation pathways. This study was designed to evaluate the hepatic and serum expression of HBsAg and its association with hepatic SORT1 gene expression in patients with chronic HBV. Thirty chronic hepatitis B patients with histological examination results were enrolled in this study. Liver biopsies were analyzed for hepatic HBsAg and SORT1 gene expression by immunohistochemistry and quantitative real time PCR (qRT-PCR), respectively. Twenty seven out of 30 (90%) liver biopsies had positive staining for HBsAg and showed a significant inverse association with hepatic SORT1 fold change gene expression (=-0.5, P=0.042). There was significant association between HBV DNA levels and HBsAg expression in hepatocyte or serum titer of HBsAg (r=0.39, P=0.029; r=0.39, P=0.032 respectively). Serum ALT was also correlated with hepatic activity index (HAI) score (=0.6, P=0.001). Inverse association between hepatic SORT1 gene expression and hepatic HBsAg expression indicates the possible role of sortilin in HBsAg particle formation.


Hasanzadeh M.,Tabriz University of Medical Sciences | Karimzadeh A.,Higher Education Institute of Rab Rashid | Karimzadeh A.,Gonabad University of Medical Sciences | Shadjou N.,Urmia University | And 5 more authors.
Materials Science and Engineering C | Year: 2016

This study reports on the synthesis and characterization of a novel nano-composite, Fe3O4 magnetic nanoparticles/graphene quantum dots (Fe3O4 MNP-GQDs), for sensing of some amino acids. For the first time, as-synthesized GQDs and Fe3O4 MNPs-GQDs was electrodeposited on the glassy carbon electrode (GCE) by cyclic voltammetry (CV) regime in the potential range from − 1.0 to 1.0 V. Fe3O4 MNP-GQDs is engineered to specifically and effectively capture and enhancement the electrochemical signals of some amino acids at physiological pH due to the synergy among GQDs and magnetic nanoparticles. We have illustrated that the obtained Fe3O4 MNPs-GQDs exhibited a much higher electroactivity individual GQDs and Fe3O4 MNPs for the electrooxidation and detection of amino acid which was about 10 fold higher than for GQDs. Magnetic and specific properties of the Fe3O4 MNP-GQDs can be exploited to capture and pre-concentration the amino acids onto its surface, which are important for detection of multi-amino acids. © 2016 Elsevier B.V.


Hasanzadeh M.,Tabriz University of Medical Sciences | Shadjou N.,Urmia University | Mokhtarzadeh A.,Gonabad University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | And 2 more authors.
Materials Science and Engineering C | Year: 2016

Graphene is a 2-D carbon nanomaterial with many distinctive properties that are electrochemically beneficial, such as large surface-to-volume ratio, lowered power usage, high conductivity and electron mobility. Graphene-based electrochemical immune-devices have recently gained much importance for detecting antigens and biomarkers responsible for cancer diagnosis. This review describes fabrication and chemical modification of the surfaces of graphene for immunesensing applications. We also present a comprehensive overview of current developments and key issues in the determination of some biological molecules with particular emphasis on evaluating the models. This review focuses mostly on new developments in the last 5 years in development of chip architecture and integration, different sensing modes that can be used in conjunction with microfluidics, and new applications that have emerged or have been demonstrated; it also aims to point out where future research can be directed to in these areas. © 2016 Elsevier B.V.


Hasanzadeh M.,Tabriz University of Medical Sciences | Karimzadeh A.,Tabriz University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | Mokhtarzadeh A.,Gonabad University of Medical Sciences | Shadjou N.,Urmia University
Analytical and Bioanalytical Electrochemistry | Year: 2016

As a laboratory technique, the analysis of amino acid plays an important role in biochemical, pharmaceutical and biomedical fields. There is, therefore, a need for the rapid and accurate analysis of amino acid. In this report, the Fe3O4 magnetic nanoparticles/graphene quantum dots (Fe3O4 MNP-GQDs) was used for the investigation of electroactive amino acids effect on the electrochemical signals of each other. This sensor used towards probing the interaction study of electroactive amino acids with each other at physiological pH (pH=7.4) by differential pulse voltammetry. The herein described approach is expected to promote the exploitation of electrochemically-based methods for the study of amino acids-amino acid interaction which is necessary in biochemical and biosensing studies. This report may open a new window to direct peptide analysis in the future. © 2016 by CEE (Center of Excellence in Electrochemistry).


PubMed | Tabriz University of Medical Sciences and Higher Education Institute of Rab Rashid
Type: Journal Article | Journal: Drug research | Year: 2016

Despite remarkable progress in cancer treatment, development of drug resistance is still a big burden to eliminate all tumor cells and a mean cause for tumor recurrence. Recent studies have been revealed the contribution of many signaling pathways in acquisition of resistance to chemotherapy. Because of its potential in maintaining the balance between cell proliferation and apoptosis, Notch signaling pathway has mean relevance to various aspects of cancer biology, from cancer stem cells to tumor immunity to multidrug resistance. Therefore, Notch signaling pathway is an attractive target for cancer therapy because targeting Notch signaling could overcome multi drug resistance (MDR). This article will provide a brief overview of the published evidences in support of Notch targeting in reverting multidrug resistance as a safer and novel approach for the improvement of tumor treatment.


PubMed | Tabriz University of Medical Sciences, Higher Education Institute of Rab Rashid, Urmia University and Gonabad University of Medical Sciences
Type: | Journal: Materials science & engineering. C, Materials for biological applications | Year: 2016

A novel nanobiopolymer film was electrodeposited on the surface of glassy carbon through cyclic voltammetry from dopamine, -cyclodextrin, and phosphate buffer solution in physiological pH (7.40). The electrochemical behavior of polydopamine-Beta-cyclodextrin modified glassy carbon electrode was investigated for electro-oxidation and determination of some amino acids (l-Cysteine, l-Tyrosine, l-Glycine, and l-Phenylalanine). The modified electrode was applied for selected amino acid detection at physiological pH using cyclic voltammetry, differential pulse voltammetry and chronoamperometry, chronocoulometery. The linear concentration range of the proposed sensor for the l-Glycine, l-Cysteine, l-Tyrosine, and l-Phenylalanine were 0.2-70, 0.06-0.2, 0.01-0.1, and 0.2-10M, while low limit of quantifications were 0.2, 0.06, 0.01, and 0.2M, respectively. The modified electrode shows many advantages as an amino acid sensor such as simple preparation method without using any specific electron transfer mediator or specific reagent, good sensitivity, short response time, and long term stability.


PubMed | Tabriz University of Medical Sciences, Gonabad University of Medical Sciences, Urmia University and Higher Education Institute of Rab Rashid
Type: | Journal: Materials science & engineering. C, Materials for biological applications | Year: 2016

This study reports on the synthesis and characterization of a novel nano-composite, Fe3O4 magnetic nanoparticles/graphene quantum dots (Fe3O4 MNP-GQDs), for sensing of some amino acids. For the first time, as-synthesized GQDs and Fe3O4 MNPs-GQDs was electrodeposited on the glassy carbon electrode (GCE) by cyclic voltammetry (CV) regime in the potential range from -1.0 to 1.0V. Fe3O4 MNP-GQDs is engineered to specifically and effectively capture and enhancement the electrochemical signals of some amino acids at physiological pH due to the synergy among GQDs and magnetic nanoparticles. We have illustrated that the obtained Fe3O4 MNPs-GQDs exhibited a much higher electroactivity individual GQDs and Fe3O4 MNPs for the electrooxidation and detection of amino acid which was about 10 fold higher than for GQDs. Magnetic and specific properties of the Fe3O4 MNP-GQDs can be exploited to capture and pre-concentration the amino acids onto its surface, which are important for detection of multi-amino acids.

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