Entity

Time filter

Source Type


Hafezi Ghahestani Z.,Payame Noor University | Alebooye Langroodi F.,Payame Noor University | Mokhtarzadeh A.,Tabriz University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | And 2 more authors.
Artificial Cells, Nanomedicine and Biotechnology | Year: 2016

Poor solubility of crocetin has limited its use as a potential chemotherapeutic agent. In this study, a crocetin nanocarrier was formulated based on poly(lactic-co-glycolic acid) (PLGA) polymer. The effect of preparation conditions on the size and entrap efficiency of PLGA nanoparticles (NPs) via single emulsion/solvent evaporation method was evaluated. Cytotoxicity of crocetin and crocetin-encapsulated PLGA NPs was assayed in MCF-7 cell line. In PLGA formulations, the optimal condition was found to be 5% polyvinyl alcohol (PVA), crocetin:polymer ratio 1:20 and dichloromethane as organic solvent. Obtained results demonstrated that the encapsulation of crocetin in PLGA significantly increased the cytotoxicity of this compound. © 2016 Informa UK Limited, trading as Taylor & Francis Group Source


Mokhtarzadeh A.,Tabriz University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | Mokhtarzadeh A.,Mashhad University of Medical Sciences | Tabarzad M.,Shahid Beheshti University of Medical Sciences | And 4 more authors.
TrAC - Trends in Analytical Chemistry | Year: 2016

The development of enhanced drug delivery systems is one of the most attractive fields of pharmaceutical sciences, as some of the highly effective chemo/biotherapeutics for cancer treatment can not be administrated due to their high toxicities for normal cells or low stability in physiological media. However, drugs that are currently not administrable will become valuable if specific cell-targeted drug carriers can protect the normal cells from adverse effects and also improve drug pharmacokinetics. Aptamers are attractive and promising biomaterials developed with high affinity and specificity against numerous valuable targets. They could act similar to monoclonal antibodies (mAbs), and offer significant advantages. Combined with aptamers, nanostructures are smart veicles with remarkable properties for drug delivery. Combination of aptamer and nanotechnology has resulted in the production of various targeted drug delivery systems which are highly efficient in therapeutic and diagnostic applications. In this review, some of the efforts related to design and development of aptamer-targeted nanocarriers have been summarized considering: i) Aptamer importance as smart ligands and the aptamer development methods ii) Types of nanostructures combined with aptamers as targeting agent proposed in the literature iii) Cancer specific aptamers evaluated in combination with nanocarriers for diagnostic and therapeutic applications and iv) Discussion of aptamer-based smart nanocarriers according to the trend of related research works. © 2016 Elsevier B.V. Source


Mokhtarzadeh A.,Tabriz University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | Mokhtarzadeh A.,Mashhad University of Medical Sciences | Alibakhshi A.,Shahid Beheshti University of Medical Sciences | And 4 more authors.
Expert Opinion on Biological Therapy | Year: 2016

Introduction: Gene therapy mainly depends on the use of appropriate delivery vehicles with no induction of immune responses and toxicity. The limitations of viral gene carriers such as induction of immunogenicity, random integration in the genome of the host, limitations in the size, has led to a movement toward non-viral systems with much safer properties. Biodegradable and biocompatible polymeric nanocarriers due to several unique properties such as excellent biocompatibility, prolonged gene circulation time, prevented gene degradation, passive targeting by using the enhanced permeability and retention (EPR) effect, and possibility of modulating polymers structure to obtain desirable therapeutic efficacy, are among the most promising systems for gene delivery. However, biodegradable gene delivery systems have some limitations such as inadequate stability and slow release of therapeutics which have to be overcome. Thus, a variety of advanced functional biodegradable delivery systems with more efficient gene delivery activity has recently been introduced. Areas covered: This review summarizes different aspects of biodegradable and biocompatible nano carriers including formulation, mechanism of intracellular uptake, various potential applications of biodegradable nanoparticles and finally recent studies on the therapeutic efficacy of these nanoparticles in sustained delivery of genes. Expert opinion: Biocompatible and biodegradable polymers will play a necessary and important role in developing new and safe carriers for oligonucleotide delivery. More working and the development of optimized polymers will reveal more their efficacy in the treatment of patients via helping in better gene therapy. © 2016 Informa UK Limited, trading as Taylor & Francis Group Source


Najafzadeh N.,Pasteur Institute of Iran | Sedaghat M.M.,Tehran University of Medical Sciences | Sultan S.S.,Tehran University of Medical Sciences | Spotin A.,Pasteur Institute of Iran | And 6 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2014

Introduction: Leishmania major is the causative agent of zoonotic cutaneous leishmaniasis (ZCL), and great gerbils are the main reservoir hosts in Iran. Abarkouh in central Iran is an emerging focal point for which the reservoir hosts of ZCL are unclear. This research project was designed to detect any Leishmania parasites in different wild rodent species. Methods: All rodents captured in 2011 and 2012 from Abarkouh district were identified based on morphological characteristics and by amplification of the rodent cytochrome b (Cyt b) gene. To detect Leishmania infection in rodents, deoxyribonucleic acid (DNA) of each ear was extracted. Internal transcribed spacer-ribosomal deoxyribonucleic acid (ITS-rDNA), microsatellites, kinetoplast deoxyribonucleic acid (kDNA) and cytochrome b genes of Leishmania parasites were amplified by polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequencing were employed to confirm the Leishmania identification. Results: Of 68 captured rodents in the region, 55 Rhombomys opimus were identified and nine Leishmania infections (9/55) were found. In addition, eight Meriones libycus and two Tatera indica were sampled, and one of each was confirmed to be infected. Two Meriones persicus and one Mus musculus were sampled with no infection. Conclusions: The results showed that all 11 unambiguously positive Leishmania infections were Leishmania major. Only one haplotype of L. major (GenBank access No. EF413075) was found and at least three rodents R. opimus, M. libycus and T. indica—appear to be the main and potential reservoir hosts in this ZCL focus. The reservoir hosts are variable and versatile in small ZCL focal locations. © 2014, Sociedade Brasileira de Medicina Tropical. All Rights reserved. Source


Mokhtarzadeh A.,Tabriz University of Medical Sciences | Mokhtarzadeh A.,Higher Education Institute of Rab Rashid | Alibakhshi A.,Shahid Beheshti University of Medical Sciences | Hejazi M.,Gonabad University of Medical Sciences | And 2 more authors.
TrAC - Trends in Analytical Chemistry | Year: 2016

There are several biocompatible and biodegradable biopolymers, which found tremendous medical and pharmaceutical applications and currently receiving unprecedented attention. Various microorganisms can synthesize a variety of these biopolymers such as polysaccharides, polyamides and polyesters, which could be used for development of new generation of drug carriers and tissue repairing materials. The size, charge, chemical structure and other physicochemical properties of bacterial biodegradable polymers represent a good compatibility in development of tissue scaffolds and also as delivery vehicles of therapeutic agents. Here, we highlight recent advances in engineering biopolymers derived from bacteria, especially for drug delivery and tissue engineering. © 2016 Elsevier B.V. Source

Discover hidden collaborations