Sousse, Tunisia
Sousse, Tunisia

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Hasnaoui N.,Higher Agronomic Institute | Hasnaoui N.,Tunis el Manar University | Mars M.,Higher Agronomic Institute | Chibani J.,Laboratory of Biochemistry and Molecular Biology | Trifi M.,Tunis el Manar University
Diversity | Year: 2010

The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD) method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are characterized by a collective resolving power of 12.83. In addition, the cluster analysis has exhibited a parsimonious tree branching independent from the geographic origin of the cultivars. In spite of the relatively low number of primers and cultivars, RAPD constitutes an appropriate procedure to assess the genetic diversity and to survey the phylogenetic relationships in this crop. © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.


Hasnaoui N.,Laboratory of Molecular Genetics | Hasnaoui N.,Higher Agronomic Institute | Buonamici A.,CNR Institute of Plant Genetics | Sebastiani F.,University of Florence | And 4 more authors.
Conservation Genetics Resources | Year: 2010

In the present work, we report the development of 11 microstallite markers (SSR) for Punica granatum. Evaluated on a set of 27 pomegranate accessions sampled in Tunisia, they displayed 25 alleles, with number of alleles per locus ranging between 1 and 4, and an observed heterozygosity from 0.037 and 0.592. This set of SSR markers can be very useful for studies dealing with genetic diversity assessment of germplasm, with cultivars/varieties fingerprinting and pedigree analysis of this economically important fruit species. © Springer Science+Business Media B.V. 2010.


Laboratory studies were conducted to determine the efficiency of Coccinella algerica Kovr (Coleoptera: Coccinellidae) against Pterochloroides persicae (Cholodkovsky) (Hemiptera: Lachninae) and compared against Acyrthosiphum pisum Harris (Hemiptera: Aphidinae). Feeding on P. persicae had a significant effect on predation rate by either fourth stage larvae or adults of C. algerica. The developmental period of C. algerica reared on P. persicae was significantly shorter than those reared on A. pisum (F = 51.45, d.f. = 1.19, P = 0.05). Larvae and adults of C. algerica reared on P. persicae showed a significantly greater mortality than those on A. pisum (F = 27.29, d.f. = 2, P = 0.05). Furthermore, the aphid species has an effect on body weight and fecundity of C. algerica. These results demonstrated that C. algerica is not recommended as a biological control agent of P. persicae in Tunisia.


Hasnaoui N.,Higher Agronomic Institute | Hasnaoui N.,Laboratory of Molecular Genetics | Mars M.,Higher Agronomic Institute | Ghaffari S.,Institute of Arid regions | And 3 more authors.
Industrial Crops and Products | Year: 2011

Tunisian pomegranate genetic resources consist of sweet and sour cultivars, showing large morphometric variability. In the present work we characterized seeds and juice contents of sugars and organic acids of 5 sour and 7 sweet pomegranate cultivars. Results showed that citric acid was predominant in sour pomegranates, while malic acid was the most prevalent in sweet ones. Paradoxically, sour cultivars have higher sugar content than the sweet ones. A strong correlation was found between sourness and citric acid content, which is assumed to be the major factor that determines sour taste in pomegranate fruits. Besides, some of the seed parameters showed a significant positive correlation with acidity. Sweet cultivars were appropriate for fresh consumption and juice production due to several attributes in addition to their sweetness. Equally, sour pomegranate showed several characteristics that could be of great interest for food and nutraceutical industries. © 2010 Elsevier B.V.


Hasnaoui N.,Higher Agronomic Institute | Hasnaoui N.,Tunis el Manar University | Jbir R.,Higher Agronomic Institute | Jbir R.,Tunis el Manar University | And 5 more authors.
International Journal of Food Properties | Year: 2011

Juices made from fruits of 30 Tunisian accessions of pomegranate were studied for their organic acids, sugars, and anthocyanin contents, using high performance liquid chromatography. Among the detected organic acids, malic acid was the major one (>50%) followed by citric acid (>22%), while among sugars, fructose and glucose were most present in pomegranate juice contributing 53.9 and 43.4% of the total sugar content, respectively. The total anthocyanin content ranged from 9-115 mg per litre of juice with the following ranges of the six compounds found: cyanidin-3,5-diglucoside (3.1-74.4 mg/L), delphinidin-3-glucoside (0.7-22.0 mg/L), cyanidin-3-glucoside (0.8-21.0 mg/L), pelargonidin-3-glucoside (0.5-16.1 mg/L), pelargonidin-3,5-diglucoside (0.0-11.8 mg/L), and delphinidin-3,5-diglucoside (0.0-5.4 mg/L). Based on the analyzed parameters, cluster analysis allowed grouping cultivars into two main clusters. One was made of sour cultivars and the second of the sweet ones. Principle component and cluster analyses suggested that the composition of the pomegranate fruits is determined by cultivar rather than cultivation location. Copyright © Taylor & Francis Group, LLC.


Hasnaoui N.,Higher Agronomic Institute | Hasnaoui N.,Laboratory of Molecular Genetics | Buonamici A.,CNR Institute of Plant Genetics | Sebastiani F.,University of Florence | And 3 more authors.
Gene | Year: 2012

Pomegranate (Punica granatum L.) is one of the oldest known edible fruits and more and more it arouse interest of scientific community given its numerous biological activities. However, information about its genetic resources and characterization using reliable molecular markers are still scarce. In the present study, we report the development of 4 new polymorphic SSR markers. They have been used in addition to 11 SSRs previously published to investigate molecular diversity of 33 P. granatum ecotypes. Based on the multi-locus profiles, twenty-two distinctive genotypes were identified. Globally, quite low genetic diversity has been revealed, as measured by allele richness (2.83 per locus) and heterozygosity (He = 0.245; Ho = 0.243), reflecting the narrow genetic background of the plant material. Four synonymous groups could be detected involving 15 accessions. Results of ordination and cluster analysis suggested that almost all the Tunisian cultivars share similar genetic background, and are likely derived from a small number of introductions in ancient times. Results issued from this study provide essential information to project a pomegranate core-collection without plant material duplication and for sustainable management of pomegranate landraces at national and international level. Furthermore, these SSR markers are powerful tool for marker assisted selection (MAS) program and for QTL studies. © 2011 Elsevier B.V.


Baraket G.,Tunis el Manar University | Chatti K.,Tunis el Manar University | Saddoud O.,Tunis el Manar University | Abdelkarim A.B.,Tunis el Manar University | And 3 more authors.
Plant Molecular Biology Reporter | Year: 2011

This study characterises the genetic variability of fig, Ficus carica L., using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. It compares the efficiency and utility of the two techniques in detecting variation and establishing genetic relationships among Tunisian fig cultivars. Our results show that using both marker systems, the Tunisian fig germ plasm is characterised by having a large genetic diversity at the deoxyribonucleic acid level, as most of AFLP bands were detected and all SSR markers were polymorphic. In fact, 351 (342 polymorphic) and 57 (57 polymorphic) bands were detected using AFLP and SSR primers, respectively. SSR markers were the most polymorphic with an average polymorphic information content value of 0.94, while AFLP markers showed the highest effective multiplex ratio (56.9) and marker index (45.2). The effective marker index was recorded highest (4.19) for AFLP markers and lowest (0.70) for the SSR ones. Our results demonstrate that (1) independent as well as combined analyses of cluster analyses of SSR and AFLP fragments showed that cultivars are clustered independently from their geographical origin, horticultural classifications and tree sex; (2) the analysis of molecular variance allowed the partitioning of genetic variation within and among fig groups and showed greater variation within groups and (3) AFLP and SSR markers datasets showed positive correlation. This study suggests the SSR and AFLP markers are suitable for diversity analysis and cultivars fingerprinting. An understanding of the genetic diversity and population structure of F. carica in Tunisia can also provide insight into the conservation and management of this species. © 2010 Springer-Verlag.


PubMed | Higher Agronomic Institute
Type: Journal Article | Journal: Gene | Year: 2012

Pomegranate (Punica granatum L.) is one of the oldest known edible fruits and more and more it arouse interest of scientific community given its numerous biological activities. However, information about its genetic resources and characterization using reliable molecular markers are still scarce. In the present study, we report the development of 4 new polymorphic SSR markers. They have been used in addition to 11 SSRs previously published to investigate molecular diversity of 33 P. granatum ecotypes. Based on the multi-locus profiles, twenty-two distinctive genotypes were identified. Globally, quite low genetic diversity has been revealed, as measured by allele richness (2.83 per locus) and heterozygosity (He=0.245; Ho=0.243), reflecting the narrow genetic background of the plant material. Four synonymous groups could be detected involving 15 accessions. Results of ordination and cluster analysis suggested that almost all the Tunisian cultivars share similar genetic background, and are likely derived from a small number of introductions in ancient times. Results issued from this study provide essential information to project a pomegranate core-collection without plant material duplication and for sustainable management of pomegranate landraces at national and international level. Furthermore, these SSR markers are powerful tool for marker assisted selection (MAS) program and for QTL studies.

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