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Chen L.,Nanjing Southeast University | Miao W.,Nanjing Southeast University | Zhang H.,Heze Hospital of Traditional Chinese Medicine | Zeng F.,Xiamen University | And 7 more authors.
Journal of Biomedical Nanotechnology | Year: 2014

We investigated the inhibitory effects of anti-Neuropilin-1 monoclonal antibody (NRP-1 MAb) on adhesion of glioma cells to Fibronectin (FN) and the associated mechanisms. The effects of NRP-1 MAb on adhesion of U251 glioma cells and formation of stress fibers were studied using adhesion assays and confocal fluorescence microscopy. After treatment of U251 glioma cells with NRP-1 MAb, changes of related proteins adhering to FN were detected by co- immunoprecipitation and Western blot. Adhesion assays showed that NRP-1 MAb played an inhibitory role in adhesion of U251 glioma cells. Confocal fluorescence microscopy, co-immunoprecipitation and Western blot showed that NRP-1 MAb can inhibit the formation of stress fibers adhering to U251 cells. NRP-1 MAb can also affect the formation of Integrin α5β1-NRP-1 complex, and also reduce the level of phosphorylation of FAK and p130cas. In conclusion, NRP-1 MAb has the inhibitory effects on adhesion of glioma cells to FN. This effect is related to the inhibition of NRP-1 MAb on the formation of Integrin α5β1-NRP-1 complex, which leads to the inhibition of FAK/p130cas signaling pathway. Copyright © 2014 American Scientific Publishers All rights reserved.


PubMed | Qingdao Jiaozhou District Central Hospital, Qingdao Huangdao District Hospital of Traditional Chinese Medicine, Xian Health Management Service Center, Juye County Peoples Hospital and 3 more.
Type: | Journal: Biochemical and biophysical research communications | Year: 2016

The regulatory transcriptional factor PATZ1 is abnormally up-regulated in diabetic endothelial cells (ECs) where it acts as an anti-angiogenic factor via modulation of fatty acid-binding protein 4 (FABP4) signaling. The aim of the present work was to elucidate the upstream molecular events regulating PATZ1 expression in diabetic angiogenesis. The bioinformatics search for microRNAs (miRNAs) able to potentially target PATZ1 led to the identification of several miRNAs. Among them we focused on the miR-24 since the multiple targets of miR-24, which have so far been identified in beta cells, cardiomyocytes and macrophages, are all involved in diabetic complications. miR-24 expression was significantly impaired in the ECs isolated from diabetic hearts. Functionally, endothelial migration was profoundly inhibited by miR-24 suppression in Ctrl ECs, whereas miR-24 overexpression by mimics treatment effectively restored the migration rate in diabetic ECs. Mechanistically, miR-24 directly targeted the 3untranslated region (3UTR) of PATZ1, and miR-24 accumulation potentiated endothelial migration by reducing the mRNA stability of PATZ1. Together, these results suggest a novel mechanism regulating endothelial PATZ1 expression based on the down-regulation of miR-24 expression caused by hyperglycemia. Interfering with PATZ1 expression via miRNAs or miRNA mimics could potentially represent a new way to target endothelial PATZ1-dependent signaling of vascular dysfunction in diabetes.


Shen Y.,Central South University | Tang M.-L.,Central South University | Tang M.-L.,Heze Hospital of Traditional Chinese Medicine | Wu X.-P.,Central South University | And 8 more authors.
Bone | Year: 2016

Femoral neck geometric parameters (FNGPs) are closely related to the strength of the femoral neck and the risk of fragility fractures. No reference database is available for FNGPs for Chinese population, and gender-related differences in FNGPs as well as their association with the risk of femoral neck fractures are unknown. This investigation aimed to set up reference databases for FNGPs, understand gender-related differences in FNGPs, and examine the association between FNGPs and the risk of osteoporotic fractures of the femoral neck. This study included 5268 females and 2156 males (aged 15–91 years) from Chinese population. A total of 384 patients (282 females and 102 males) had sustained femoral neck fractures; 384 age- and sex-matched individuals without any fractures served as controls. Femoral neck DXA images were used to measure bone mineral density (BMD) and eight FNGPs. Our results showed that the age-related trends of FNGPs were fitted with the best goodness-of-fit by applying the cubic regression model. The trends shown by FNGPs were significantly different between male and female subjects, and the fitting curves were significantly higher in male subjects. After adjustments were made for age, height, weight, and body mass index, Cox regression analysis showed that changes in all FNGPs were related to increased hazard ratios (HRs) of femoral neck fractures. After further adjustment was made for BMD of the femoral neck, the HRs related to a cortical thickness (CT) decrease and buckling ratio (BR) increase in females went up by 3.35-folds (95% CI: 2.75–4.07) and 1.86-folds (95% CI: 1.33–2.60), respectively. In males, the HRs related to the decrease in CT and cross-sectional area (CSA) increased by 3.21-folds (95% CI: 2.32–4.45) and 1.88-folds (95% CI: 1.03–3.44), respectively. In conclusions, the reference databases of FNGPs established in this study will assist in the evaluation and prediction of femoral neck fracture risk in the clinic. The decrease in CT and increase in BR of the femoral neck were independent risk factors for osteoporotic fractures of the femoral neck in females from mainland China, while a decrease in CT and CSA were risk factors in male. © 2016 Elsevier Inc.


Sun J.,Anhui Medical University | Sun J.,Heze Hospital of Traditional Chinese Medicine | Yu N.,Anhui Medical University | Yu N.,University of Southern California | And 6 more authors.
International Journal of Rheumatic Diseases | Year: 2016

Aim: The dysfunction of T regulatory cells is important for the pathogenesis of systemic lupus erythematosus (SLE). Glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR) is expressed at low levels on resting responder T lymphocytes (Tresps) and is up-regulated on T regulatory cells (Tregs) and activated T cells, diminishing suppressive activity of Tregs and/or leading to resistance to suppression of Tregs by activated effector T cells. We aimed to explore whether SLE patients had an aberrant expression of GITR on Tregs and responder T cells (Tresps) and the regulation by glucocorticoids. Methods: The surface GITR expression on Tregs and Tresps cells were analyzed by flow cytometry in 32 patients and 15 normal controls. Purified Tregs or Tresps were cultured with glucocorticoid. Apoptosis of the cells were determined by the staining of Annexin V. Results: Systemic lupus erythematosus patients had higher levels of GITR expressed on CD4+CD25+, CD4+CD25high and CD4+CD25+CD127low/- Tregs as well as on CD4+CD25- Tresps compared to healthy controls. The expression of GITR on Tregs and Tresps were positively correlated with score of SLE disease activity index (SLEDAI). In vitro glucocorticoid induced GITR expression on purified Tresp cells, but not on Tregs, and almost all of the GITR positive cells induced by glucocorticoid encountered apoptosis. Conclusion: Aberrant expression of GITR may contribute to SLE pathogenesis. Glucocorticoid may achieve its therapeutic effect partly by inducing GITR expression on Tresps rather than Tregs, which initiates the apoptosis of Tresp cells in SLE patients. © 2016 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Ltd.


Zhang Z.Y.,Heze Hospital of Traditional Chinese Medicine | Xu R.Q.,Heze Hospital of Traditional Chinese Medicine | Guo T.J.,Heze Hospital of Traditional Chinese Medicine | Zhang M.,Heze Hospital of Traditional Chinese Medicine | And 2 more authors.
Genetics and Molecular Research | Year: 2014

The purpose of this study was to identify differentially expressed genes and analyze biological processes related to leukemia. A meta-analysis was performed using the Rank Product package of Gene Expression Omnibus datasets for leukemia. Next, Gene Ontology-enrichment analysis and pathway analysis were performed using the Gene Ontology website and Kyoto Encyclopedia of Genes and Genomes. A protein-protein interaction network was constructed using the Cytoscape software. Using the Rank Product package for leukemia, we identified a total of 1294 differentially expressed genes, 357 of which were not involved in individual differentially expressed genes. Gene Ontology-enrichment analyses showed that these 357 genes were enriched in biological processes such as mRNA metabolism, RNA splicing, and mRNA processing. Pathway-enrichment analysis showed that the genes were involved in the intestinal immune network for IgA production, endocytosis, and the mitogen-activated protein kinase signaling pathway. The protein-protein interaction network indicated that HRAS, CD44, STAT1, SMAD2, and COPS5 were important in many interactions. Our study revealed genes that were consistently differentially expressed in leukemia, as well as the biological pathways and protein-protein interaction network associated with these genes. © FUNPEC-RP.


PubMed | Heze Hospital of Traditional Chinese Medicine
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2014

The purpose of this study was to identify differentially expressed genes and analyze biological processes related to leukemia. A meta-analysis was performed using the Rank Product package of Gene Expression Omnibus datasets for leukemia. Next, Gene Ontology-enrichment analysis and pathway analysis were performed using the Gene Ontology website and Kyoto Encyclopedia of Genes and Genomes. A protein-protein interaction network was constructed using the Cytoscape software. Using the Rank Product package for leukemia, we identified a total of 1294 differentially expressed genes, 357 of which were not involved in individual differentially expressed genes. Gene Ontology-enrichment analyses showed that these 357 genes were enriched in biological processes such as mRNA metabolism, RNA splicing, and mRNA processing. Pathway-enrichment analysis showed that the genes were involved in the intestinal immune network for IgA production, endocytosis, and the mitogen-activated protein kinase signaling pathway. The protein-protein interaction network indicated that HRAS, CD44, STAT1, SMAD2, and COPS5 were important in many interactions. Our study revealed genes that were consistently differentially expressed in leukemia, as well as the biological pathways and protein-protein interaction network associated with these genes.

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