Chen L.,Nanjing Southeast University |
Miao W.,Nanjing Southeast University |
Zhang H.,Heze Hospital of Traditional Chinese Medicine |
Zeng F.,Xiamen University |
And 7 more authors.
Journal of Biomedical Nanotechnology | Year: 2014
We investigated the inhibitory effects of anti-Neuropilin-1 monoclonal antibody (NRP-1 MAb) on adhesion of glioma cells to Fibronectin (FN) and the associated mechanisms. The effects of NRP-1 MAb on adhesion of U251 glioma cells and formation of stress fibers were studied using adhesion assays and confocal fluorescence microscopy. After treatment of U251 glioma cells with NRP-1 MAb, changes of related proteins adhering to FN were detected by co- immunoprecipitation and Western blot. Adhesion assays showed that NRP-1 MAb played an inhibitory role in adhesion of U251 glioma cells. Confocal fluorescence microscopy, co-immunoprecipitation and Western blot showed that NRP-1 MAb can inhibit the formation of stress fibers adhering to U251 cells. NRP-1 MAb can also affect the formation of Integrin α5β1-NRP-1 complex, and also reduce the level of phosphorylation of FAK and p130cas. In conclusion, NRP-1 MAb has the inhibitory effects on adhesion of glioma cells to FN. This effect is related to the inhibition of NRP-1 MAb on the formation of Integrin α5β1-NRP-1 complex, which leads to the inhibition of FAK/p130cas signaling pathway. Copyright © 2014 American Scientific Publishers All rights reserved.
Zhang Z.Y.,Heze Hospital of Traditional Chinese Medicine |
Xu R.Q.,Heze Hospital of Traditional Chinese Medicine |
Guo T.J.,Heze Hospital of Traditional Chinese Medicine |
Zhang M.,Heze Hospital of Traditional Chinese Medicine |
And 2 more authors.
Genetics and Molecular Research | Year: 2014
The purpose of this study was to identify differentially expressed genes and analyze biological processes related to leukemia. A meta-analysis was performed using the Rank Product package of Gene Expression Omnibus datasets for leukemia. Next, Gene Ontology-enrichment analysis and pathway analysis were performed using the Gene Ontology website and Kyoto Encyclopedia of Genes and Genomes. A protein-protein interaction network was constructed using the Cytoscape software. Using the Rank Product package for leukemia, we identified a total of 1294 differentially expressed genes, 357 of which were not involved in individual differentially expressed genes. Gene Ontology-enrichment analyses showed that these 357 genes were enriched in biological processes such as mRNA metabolism, RNA splicing, and mRNA processing. Pathway-enrichment analysis showed that the genes were involved in the intestinal immune network for IgA production, endocytosis, and the mitogen-activated protein kinase signaling pathway. The protein-protein interaction network indicated that HRAS, CD44, STAT1, SMAD2, and COPS5 were important in many interactions. Our study revealed genes that were consistently differentially expressed in leukemia, as well as the biological pathways and protein-protein interaction network associated with these genes. © FUNPEC-RP.