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Zheng C.-X.,Sun Yat Sen University | Wang Z.-Q.,Sun Yat Sen University | Lin W.-B.,Hexian Memorial Hospital | Chu Z.-H.,Sun Yat Sen University | And 2 more authors.
Chinese Medical Journal | Year: 2011

Background Little attention has been paid to the expression of heat shock protein 27 (HSP27) in patients with reflux esophagitis (RE), and few studies of the importance of HSP27 in esophagitis have been carried out in animal models. This study aimed to explore the expression of HSP27 in the esophageal tissue of rats with RE. Methods Eighty female Wistar rats were randomly divided into experimental groups A and B and control groups C and D (n=20 in each group). To establish RE, rats in the two experimental groups received pylorus and forestomach ligations, while rats in the control group received gastrostomy and gastric perforation repair. The rats in groups A and C were sacrificed 7 days after surgery, and the rats in groups B and D were sacrificed 14 days after surgery. In groups A and B, 10 and 8 rats were diagnosed with RE by pathological examination, respectively (they were included in groups A' and B', respectively). The histopathological diagnosis of all the lower esophageal tissues in groups C and D was normal and 20 normal specimens were randomly selected for groups C' and D' with 10 specimens in each group. Macroscopic and microscopic esophagitis scores were assessed for the specimens in groups A' and B'. Lower esophageal tissues were collected from groups A', B', C', and D', and paraffin-embedded slices were made using part of the tissues. The expression of HSP27 in the tissues was detected using the two-step streptavidin-peroxidase immunohistochemical method. Some collected tissues were frozen, and expressions of HSP27 mRNA were detected using fluorescence quantitative polymerase chain reaction (FQ-PCR). Results Median macroscopic and microscopic esophagitis scores in groups A' (n=10) and B' (n=8) were 1.0 and 1.5, and 2.0 and 2.5, respectively. There were no significant differences in the macroscopic or microscopic esophagitis scores between the two groups (Z=-0.330, P=0.741; Z=-0.142, P=0.887, respectively). Immunohistochemical staining showed that HSP27 was expressed in all layers of the esophageal epithelia in RE and control rats. FQ-PCR showed that HSP27 mRNA levels in the lower esophageal tissue in RE group (groups A' and B') were higher than those in control group (groups C' and D') (Z=-0.249, P=0.001). HSP27 mRNA expression in the lower esophageal tissue was significantly different in groups B' and D' (Z=-3.027, P=0.002). And the levels of HSP27 mRNA expression in severe RE group (microscopic esophagitis score: 3) were higher than in mild RE group (microscopic esophagitis score: 1-2) and control group (Z=-3.396,P=0.001; Z=-3.855, P <0.001). Conclusions HSP27 mRNA expression in the lower esophageal tissue of rats with RE is significantly higher than in the normal controls. Although reflux is a persistent stimulating factor, increased expression of HSP27 in the lower esophageal tissue of rats with RE requires aggravated esophageal injury.

Qu Y.,Guangdong Academy of Medical science | Liu X.,Guangdong Academy of Medical science | Zhuang J.,Surgery Academy | Chen G.,Dongguan Houjie Hospital | And 8 more authors.
PLoS ONE | Year: 2016

There are 16.5 million newborns in China annually. However, the incidence of congenital heart disease (CHD) has not been evaluated. In 2004, we launched an active province-wide hospital-based CHD registry in the Guangdong Province of southern China. In this study, we examined the incidence of CHD and its subtypes from 2004 to 2012 and compared our findings to the literature. Our results indicate there is an increasing trend of CHD incidence. The increase in incidence occurred mainly for single lesion and the most common subtypes (e.g., ventricular or atrial septal defect, patent ductus arteriosus). There were no increases found for multiple lesions or more complex subtypes. The proportion of CHD cases that were detected early (e.g., 1 week) increased over time. The incidence of CHD stabilized in 2010-2012 with the average cumulative incidences of 9.7, 9.9, and 11.1 per 1,000 live births at 1 week, 1 month, and 1 year, respectively. The incidences of CHD subtypes were comparable with recent international results. The data did not support previous reports that Asian children have a higher incidence of pulmonary outflow obstructions and lower incidence of transposition of the great arteries. However, there was a lower incidence of left ventricular outflow tract obstructions observed in our series. The increase in CHD incidence observed over time was due to improved detection and diagnosis. The true incidence of CHD in China was approximately 11.1 per 1,000 live births, which is higher than previously reported. © 2016 Qu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Zheng C.-X.,Sun Yat Sen University | Lin W.-B.,Hexian Memorial Hospital | Yu J.-F.,Sun Yat Sen University | Chen L.-H.,Sun Yat Sen University
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010

BACKGROUND: Partial pylorus ligation is a common method for establishment of reflux esophagitis (RE) model. The diameter of pylorus ligation is closely related to the efficacy of establishment. OBJECTIVE: To investigate the diversity of partial pylorus ligation with different diameters of stents plus forestomach ligation and to find a rational method to induce RE in rats. MATERIALS: Forty female Wistar rats were randomly divided into 2 groups, and 2.9 mm diameter and 3.7 mm diameter stents were inserted through the incision to sustain the pylorus and extracted after pylorus ligation. Then ligation of the transitional region between the forestomach and stomach was performed additionally to establish RE model. The body weights and 7-day survival rates of rats in each group were observed. The rats were sacrificed 7 days after the procedures, and the incidences of esophagitis of two groups were counted. RESULTS AND CONCLUSION: The 7-day survival rates of 2.9 mm diameter and 3.7 mm diameter stents groups were 35% (7/20) and 68.4% (13/19), respectively (P = 0.038). The incidences of esophagitis were 85.7% (6/7) and 76.9% (10/13), respectively. There were no significant differences in the body weights of everyday postoperatively in survival rats between two groups (P > 0.05). Successful RE model in rats can be established by partial pylorus ligation plus forestomach ligation. The pylorus ligation diameter is closely related to the survival rates of rats.

Du W.,General Hospital of Guangzhou Military Command | Ye D.,Hexian Memorial Hospital | Wang Y.,Southern Medical University | Gong X.,Karolinska Institutet
American Journal of Chinese Medicine | Year: 2016

Inhaled corticosteroids (ICS) are widely used to manage chronic obstructive pulmonary disease (COPD). However, withdrawal of ICS generally causes various adverse effects, warranting careful management of the ICS withdrawal. Pinellia ternata, a traditional Chinese herbal medicine, has been used to treat respiratory diseases in China for centuries. Here, we investigated its role in antagonizing ICS withdrawal-induced side effects, and explored the underlying mechanisms. The rat COPD model was established using a combination of passive cigarette smoking and intratracheal instillation of lipopolysaccharide (LPS). COPD rats were treated with saline or budesonide inhalation, or with budesonide inhalation followed by saline inhalation or Pinellia ternata gavage. The number of goblet cells and the level of mucin 5AC (MUC5AC) were enhanced by budesonide withdrawal. Pinellia ternata treatment significantly blocked these effects. Further, Pinellia ternata treatment reversed budesonide withdrawal-induced increase of interleukin 1(Formula presented.) (IL-1(Formula presented.) and tumor necrosis factor (Formula presented.) (TNF-(Formula presented.)) levels in bronchoalveolar lavage fluid (BALF). Extracellular signal-regulated kinase (ERK), but neither p38 nor c-Jun N-terminal kinase (JNK), was activated by budesonide withdrawal, and the activation was blocked by Pinellia ternata treatment. The MUC5AC expression was positively correlated with goblet cell number, IL-1(Formula presented.) and TNF-(Formula presented.) levels, and ERK activity. Pinellia ternata treatment protected the airway from ICS withdrawal-induced mucus hypersecretion and airway inflammation by inhibiting ERK activation. Pinellia ternata treatment may represent a novel therapeutic strategy to prevent ICS withdrawal-induced side effects in COPD patients. © 2016 World Scientific Publishing Company

Xie Q.,Hexian Memorial Hospital | Li X.-X.,Laizhou Peoples Hospital | Zhang P.,Hexian Memorial Hospital | Li J.-C.,Hexian Memorial Hospital | And 5 more authors.
Molecular Medicine Reports | Year: 2014

Ischemia or hypoxia-induced myocardial injury is closely associated with oxidative stress. Scavenging free radicals and/or enhancing endogenous antioxidative defense systems may be beneficial for the impediment of myocardial ischemic injury. Hydrogen (H2) gas, as a water- and lipid-soluble small molecule, is not only able to selectively eliminate hydroxyl (·OH) free radicals, but also to enhance endogenous antioxidative defense systems in rat lungs and arabidopsis plants. However, thus far, it has remained elusive whether H2 gas protects cardiomyocytes through enhancement of endogenous antioxidative defense systems. In the present study, the cardioprotective effect of H2 gas against ischemic or hypoxic injury was investigated, along with the underlying molecular mechanisms. H9c2 cardiomyoblasts (H9c2 cells) were treated in vitro with a chemical hypoxia inducer, cobalt chloride (CoCl2), to imitate hypoxia, or by serum and glucose deprivation (SGD) to imitate ischemia. Cell viability and intracellular ·OH free radicals were assessed. The role of an endogenous antioxidative defense system, the NF-E2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) signaling pathway, was evaluated. The findings revealed that treatment with CoCl2 or SGD markedly reduced cell viability in H9c2 cells. H 2 gas-rich medium protected against cell injury induced by SGD, but not that induced by CoCl2. When the cells were exposed to SGD, levels of intracellular ·OH free radicals were markedly increased; this was mitigated by H2 gas-rich medium. Exposure of the cells to SGD also resulted in significant increases in HO-1 expression and nuclear Nrf2 levels, and the HO-1 inhibitor ZnPP IX and the Nrf2 inhibitor brusatol aggravated SGD-induced cellular injury. H2 gas-rich medium enhanced SGD-induced upregulation of HO-1 and Nrf2, and the HO-1 or Nrf2 inhibition partially suppressed H2 gas-induced cardioprotection. Furthermore, following genetic silencing of Nrf2 by RNA interference, the effects of H2 gas on the induction of HO-1 and cardioprotection were markedly reduced. In conclusion, H2 gas protected cardiomyocytes from ischemia-induced myocardial injury through elimination of ·OH free radicals and also through activation of the Nrf2/HO-1 signaling pathway.

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