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Akhidova E.V.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Volkova T.D.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Koroev D.O.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Yakupov I.Yu.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | And 6 more authors.
Russian Journal of Bioorganic Chemistry | Year: 2013

Tumor-associated protein survivin is a bifunctional protein that can participate either in the regulation of cell division or in the inhibition of apoptosis depending on its location in the cell and its structural state. The aim of the present work was to obtain monospecific antibodies to survivin targeting various sites in the protein and suitable for the study of its structural and functional features. The ability of the antibodies to detect survivin in tumor cells and mammary tumor tissues has been assessed. Antibodies targeting the (1-22) and (95-105) fragments of the protein have been shown to have the highest specific activity. Antibodies targeting the (1-22) site preferentially bound to survivin-containing complex (presumably a dimer of survivin) in immunoblotting, whereas antibodies targeting the (95-105) site detected only the monomeric form of the protein. Analysis of malignant mammary tumor samples showed that monomeric survivin was present only in tumor tissue samples, whereas survivin-containing complex was expressed both in tumor tissues and in the tissues adjacent to the tumor. Antibodies targeting the (1-22) site were shown to preferentially detect survivin localized in the cell nuclei (and involved in the regulation of mitosis) in immunocytochemistry, while those targeting the (95-105) site stained the nucleoplasm and cytoplasm at all stages of the cell cycle. Therefore, the antibodies obtained can serve as a useful tool for structural and functional research on survivin. © 2013 Pleiades Publishing, Ltd.

Mestechkina N.M.,RAS A.N. Bach Institute of Biochemistry | Bezborodova O.A.,Herzen Cancer Research Institute | Il'ina A.V.,Russian Academy of Sciences | Levov A.N.,Russian Academy of Sciences | And 5 more authors.
Applied Biochemistry and Microbiology | Year: 2011

The influence of neutral and ionic polysaccharides on the antioxidant (AOA) and detoxifying activities of lactoferrin (LF) and the duration of its circulation in the body was studied. In addition to natural polymers, we studied chitosan synthetic derivatives with different functional groups. On the basis of AOA test, five polysaccharides were selected. The study of the detoxifying effect of LF in two models of induced toxicity revealed polysaccharides that maintained or increased the detoxifying activity of LF. We established that the formation of a complex of lactoferrin with two galactomannans and succinyl chitosan caused positive changes in LF properties: the detoxifying activity of the protein remained unchanged or increased, whereas its elimination from the body was decelerated. © 2011 Pleiades Publishing, Ltd.

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