Jones T.G.,Heritage Labs International LLC |
Warber K.D.,Heritage Labs International LLC |
Roberts B.D.,Heritage Labs International LLC
Journal of Diabetes Science and Technology | Year: 2010
Background: Hemoglobin A1c (HbA1c) has been endorsed as a tool for the diagnosis of diabetes. This test requires instrumentation that may not be available in underdeveloped areas. Dried blood spot (DBS) samples collected by finger stick procedures offer a mechanism to transport samples to laboratories that do measure HbA1c. Methods: Whole blood (ethylenediaminetetraacetic acid) was applied to Ahlstrom 226 filter paper. These DBS samples were compared to whole blood samples using the Roche Tina-quant® II immunoturbidometric assay. Hemoglobin A1c stability on DBS was assessed at three temperatures - 4, 25, and 40°C - for up to 9 days. A 44-day study was also done for DBS at 20-25°C. Results: The Tina-quant® II DBS method showed excellent agreement with whole blood HbA1c results (r2 = 0.99) with a slight positive mean bias of 0.08 ± 0.04% HbA1c (95% confidence interval). The variation in HbA1c on DBS samples subjected to different temperatures and times did not exceed 5.6%. Conclusions: Dried blood spot samples represent an alternative to whole blood for HbA1c by measurement when transporting whole blood is not feasible. © Diabetes Technology Society.