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Karachi, Pakistan

Siddiqui F.,University of Karachi | Abidi L.,University of Karachi | Lubna,University of Karachi | Poh C.F.,Niger Delta University | And 4 more authors.
Records of Natural Products | Year: 2016

Opuntia dillenii (Nagphana) traditionally used against inflammation and also possess analgesic effect. Thus in the present study analgesic properties of O. dillenii cladode methanol extract, its fractions obtained via vacuum liquid chromatography along with isolated α-pyrones, opuntiol and its glucoside, opuntioside were analyzed. The acetic acid-induced writhes were reduced by O. dillenii test agents with opuntioside being most effective (IC50 26 ± 0.9 mg/kg) and equipotent to diclofenac and β-sitosterol. Consistently, it also elicited most potent effect (IC50: 28 ± 1.1 and 24 ± 1.2 mg/kg) during early and late phases of formalin-induced paw licking, producing effect similar to diclofenac and indomethacin. It was also most effective in hot plate test. Naloxone (opioid antagonist) reversed the analgesic effects of extract and fractions but failed to antagonize the opuntiol and opuntioside analgesic effects. In conclusion, edible O. dillenii extract, its fractions, opuntiol and opuntioside reduced peripheral and centrally mediated pain via opioid dependent and independent systems. Among them opuntioside emerged as most effective analgesic possibly due to the presence of glucose moiety at position 7 of its α-pyrone ring. This is the first report of opuntiol and opuntioside analgesic effect which may serve as lead compounds in designing of new analgesics. © 2016 ACG Publications. All rights reserved.


Sheikh Z.A.,Herbion Pakistan Pvt. Ltd. | Shakeel S.,Dow University of Health Sciences | Gul S.,Jinnah University for Women | Zahoor A.,Herbion Pakistan Pvt. Ltd. | And 4 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2015

Objective: To explore the quantitative estimation of biomarkers gallic acid and berberine in polyherbal formulation Entoban syrup. Methods: High performance thin layer chromatography was performed to evaluate the presence of gallic acid and berberine employing toluene: ethyl acetate: formic acid: methanol 12:9:4:0.5 (v/v/v/v) and ethanol: water: formic acid 90:9:1 (v/v/v), as a mobile phase respectively. Results: The Rf values (0.58) for gallic acid and (0.76) for berberine in both sample and reference standard were found comparable under UV light at 273 nm and 366 nm respectively. The high performance thin layer chromatography method developed for quantization was simple, accurate and specific. Conclusions: The present standardization provides specific and accurate tool to develop qualifications for identity, transparency and reproducibility of biomarkers in Entoban syrup. © 2015 Hainan Medical University.


Shakeel S.,Dow University of Health Sciences | Gul S.,Dow University of Health Sciences | Zahoor A.,Dow University of Health Sciences | Khan S.S.,Herbion Pakistan Pvt. Ltd. | And 3 more authors.
Journal of Planar Chromatography - Modern TLC | Year: 2015

The technological improvement in the structural elucidation of natural compounds has made it probable to generate appropriate strategies for the analysis and standardization of plant-based medicines. An appliance of highly oriented hyphenated techniques provides a definite tool for herbal investigations. Therefore, the present study was directed towards the standardization of biomarkers gallic acid and berberine in polyherbal formulation Entoban capsules to ensure the quality of the herbal drugs. A rapid, simple, accurate, and specific high-performance thin-layer chromatography (HPTLC) method for the quantitative estimation of biomarkers berberine and gallic acid has been developed. HPTLC was performed to evaluate the presence of gallic acid and berberine applying toulene-ethyl acetate-formic acid-methanol (12:9:4:0.5 v/v) and ethanol-water-formic acid (90:9:1 v/v), as the mobile phase, respectively. The Rf values (0.58 for gallic acid and 0.76 for berberine) in both sample and reference standard were found comparable under ultraviolet (UV) light at 273 nm and 366 nm, respectively. The method developed resulted in good-quality peak shape and enabled high-quality resolution of biomarkers. The present standardization undertaken reveals compliance with the analytical procedure; therefore, it is concluded that Entoban capsule is a well-standardized product. Standardization falls under the specific guidelines of quality herbal medicine following the prerequisite for global harmonization.

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