Herbal Garden and Herbarium Research Institute in Indian System of Medicine

Rāmganj Mandi, India

Herbal Garden and Herbarium Research Institute in Indian System of Medicine

Rāmganj Mandi, India
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Guleria S.,Jammu University | Tiku A.K.,Jammu University | Singh G.,Jammu University | Rana S.,Herbal Garden and Herbarium Research Institute in Indian System of Medicine
Indian Journal of Agricultural Biochemistry | Year: 2011

In search of new sources of antioxidant compounds, the antioxidant activities of 20 selected medicinal plants were determined using ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays, and the total phenolic contents of these plants were measured by Folin-Ciocalteu method. Plants were extracted by the traditional method, boiling in water and also in methanol. Significant and linear correlation between antioxidant activity and phenolic content was observed in both aqueous (R 2 FRAP = 0.8889; R 2 DPPH = 0.8271) and methanol (R 2 FRAP = 0.9109; R 2 DPPH = 0.8932) extracts. Phenolic compounds are thus major contributor of antioxidant activities of these plants. Comparison of extraction efficiency of the two methods revealed that the methanol method extracted phenolic compounds efficiently, and antioxidant activity of extract was higher. Medicinal plants Syzygium cumini, Ocimum sanctum, Picrorhiza kurroa, Cinnamomum camphora and Cinnamomum tamala showed highest antioxidant activities and thus could be potential rich sources of natural antioxidants.


Guleria S.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Tiku A.K.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Singh G.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Koul A.,Sher-e-Kashmir University of Agricultural Sciences and Technology | And 2 more authors.
Journal of Plant Biochemistry and Biotechnology | Year: 2013

Antioxidant activities and phenolic contents of 26 species extracts from 20 botanical families grown in north-western Himalaya were investigated. Antioxidant activities were determined using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging and ferric reducing antioxidant power (FRAP) assays. Total phenolic content (TPC) was determined using a Folin-Ciocalteu assay. Quantitative and qualitative analysis of phenolic compounds was also carried out by reverse phase high performance liquid chromatography (RP-HPLC) using diode array detector (DAD). Major phenolics determined using RP-HPLC in analyzed species were gallic acid, chlorogenic acid, p-hydroxy benzoic acid, caffeic acid, vanillic acid, syringic acid, p-coumaric acid and ferulic acid. Antiradical efficiency (1/EC50) determined using DPPH radical scavenging assay ranged from 0. 13 to 5. 46. FRAP values ranged from 8. 66 to 380. 9 μmol Fe(II)/g dw. Similarly, the total phenolic content in the analyzed species varied from 3. 01 to 69. 96 mg of gallic acid equivalents (GAE)/g dry weight. Gallic acid was found in the majority of the samples, being most abundant compound in Syzygium cumini bark (92. 64 mg/100 g dw). Vanillic acid was the predominant phenolic compound in Picrorhiza kurroa root stolen (161. 2 mg/100 g dry weight). The medicinal plants with highest antioxidant activities were Taxus baccata and Syzygium cumini. A significant positive correlation, R2 = 0. 9461 and R2 = 0. 9112 was observed between TPC determined using Folin-Ciocalteu method and antiradical efficiency and FRAP values respectively, indicating that phenolic compounds are the major contributor of antioxidant activity of these medicinal plants. © 2012 Society for Plant Biochemistry and Biotechnology.


Guleria S.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Tiku A.K.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Rana S.,Herbal Garden and Herbarium Research Institute in Indian System of Medicine
Indian Journal of Biochemistry and Biophysics | Year: 2010

Terminalia bellerica Roxb. (Family: Combretaceae) has been valued in Indian system of medicine for treatment of wide range of diseases and reported to have antioxidant properties. In the present study, the free radical scavenging activity and antioxidant potential of acetone extract/fractions of its fruit was investigated using in vitro assays, including scavenging ability against 2, 2'-diphenyl-2-picrylhydrazyl (DPPH), β-carotene bleaching inhibition, reducing power and chelating ability on Fe2+ ions. The fruit powder was extracted at room temperature with different solvents in the order of increasing and decreasing polarity to obtain crude acetone extract which was further partitioned with ethyl acetate and water (1:1). It was found that ethyl acetate fraction was more effective than crude acetone extract in all antioxidant assays, except chelating power which was highest in water fraction. Maximum antioxidant activities (expressed as EC50 values) observed were 14.56 μg/ml, 27.81 μg/ml and 67.8 μg/ml in DPPH, β-carotene bleaching and reducing power assays, respectively. The antioxidant potential was compared with known antioxidant (butylated hydroxyl toluene) and correlated with total phenolic and flavonoid content in crude extract and fractions. Fractions rich in polyphenolic content were more effective than the crude extract.

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