Liu H.-B.,Peoples Hospital of Hengshui |
Wang W.-Z.,Peoples Hospital of Hengshui |
Liu Y.-C.,Fourth Peoples Hospital of Hengshui |
Liu X.-G.,Fourth Peoples Hospital of Hengshui |
Qiu L.,Peoples Hospital of Hengshui
Chinese Journal of Cancer Prevention and Treatment | Year: 2016
OBJECTIVE: Immunohistochemical double-staining is a advanced staining method, which can detect two different antigens' expression in one tissue section. Using this stain method to study the clinicopathologic significance, immunohistochemical phenotypes and differential diagnoses of primary adenosquamous carcinoma of the breast. METHODS: Using immunohistochemical method(SP) and (CK5/p63) immunohistochemical double stain to detect the expression of SM-MHC, p63, CK5, CK34βE12, EGFR, p53, ER, PR and C-erbB-2 in 4 cases who were diagnosed as primary adenosquamous carcinoma of the breast in the People's Hospital of Hengshui from January 1, 2011 to December 31, 2015, and the relevant literatures were also reviewed. RESULTS: Adenosquamous carcinoma of the breast accounted for 0.408%(4/979) of all breast cancer, with a median age of 55 years old. Four patients were all manifesting as painless mass. Two patients were in stage I, one in stage II A and the other in stage II B. Four patients were diagnosed breast cancer by B ultrasound, surgical procedure and intra-operative frozen section diagnosis, then underwent the modified radical mastectomy. After operation, one patient received pirarubicin plus docetaxel therapy, another patient received cyclophosphamide plus pirarubicin therapy. One patient was death after operation for only living nine days. The patient with strongly positive C-erbB-2 first received pirarubicin plus docetaxel therapy, and then targeted therapy. The follow-up ended in January 31, 2016, all of 3 cases had no metastasis and recurrence. Immunohistochemical double stain was successful. CONCLUSIONS: The incidence of primary adenosquamous carcinoma of the breast is rare, which is one subtype of metaplastic breast carcinoma. Cystic solid mass is its remarkable feature. The group of SM-MHC, p63, CK5 and CK34βE12 in immunohistochemical staining can help the diagnosis. Combination of mastectomy and adjuvant therapy are needed for patients. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.
Yu G.,Peoples Hospital of Hengshui |
Wang L.,Peoples Hospital of Hengshui |
Li Y.,Third Peoples Hospital of Hebei Province |
Ma Z.,Second Peoples Hospital of Tangshan |
Li Y.,Peoples Hospital of Hengshui
European Journal of Medical Research | Year: 2013
Background: Osteoporosis is a condition of bones that leads to an increased susceptibility to fracture and consequent painful morbidity. It has become a major issue of life quality worldwide. However, until now, the molecular mechanism of this disease is far from being clear. Methods: In this study, we obtained the gene expression profile of osteoporosis and controls from Gene Expression Omnibus and identified differentially expressed genes (DEGs) using classical t-Test method. Then, functional enrichment analyses were performed to identify the dysregulated Gene Ontology categories and dysfunctional pathways in osteoporosis patients compared to controls. Besides, the connectivity map was used to identify compounds that induced inverse gene changes to osteoporosis. Results: A total of 5581 DEGs were identified. We found these DEGs were enriched in 9 pathways by pathway enrichment analysis, including focal adhesion and MAPK signaling pathway. Besides, sanguinarine was identified as a potential therapeutic drug candidate capable of targeting osteoporosis. Conclusion: Although candidate agents identified by our approach may be premature for clinical trials, it is clearly a direction that warrants additional consideration. © 2013 Yu et al.; licensee BioMed Central Ltd.