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Liu F.-L.,Hengshui Central Blood Station | Liu N.,Hengshui Central Blood Station | Li C.-W.,Hengshui Central Blood Station
Chinese Journal of Biologicals | Year: 2011

Objective: To analyze the clinical significance of irregular antibodies in Rh blood group system. Methods: A patient carrying anti-Ce combined antibody was subjected to blood grouping by saline method as well as direct antiglobulin test, then determined for antibody potency by antiglobulin test and for combined antibody by absorption elution test, based on which blood matching was performed by saline method, antiglobulin method and polybrene method. Results: The serum antibody of the said patient was identified, based on which blood matching was successfully performed. No adverse reaction was observed after blood transfusion, and the curative effect was satisfactory. Conclusion: Besides the routine antibody screening and blood matching, it is recommended to determine various factor other than RhD for the patients needing regular blood transfusion and the women without history of giving birth to ensure the compatible transfusion of Rh system and avoid the irregular antibodies so as to ensure the safety of clinical blood transfusion.


Liu F.-L.,Hengshui Central Blood Station | Du X.-N.,Hengshui Central Blood Station | Sun J.-H.,Hengshui Central Blood Station | Liu N.,Hengshui Central Blood Station | And 2 more authors.
Chinese Journal of Biologicals | Year: 2012

Objective: To develop a method and a standard for quality control of inverse ABO blood grouping reagents. Methods: Plasma samples of group O were determined by anti-P, anti-M, anti-N, anti-C, anti-c, anti-E and anti-e reagents respectively, of which two strong positive samples with reaction results of ++ with anti-P, anti-M, anti-C and anti-e reagents and two strong positive samples with reaction results of ++ anti-P, anti-N, anti-c and anti-E reagents were selected to prepare blood cells of group O for inverse ABO blood grouping. The blood cells of group O for inverse ABO blood grouping, manufactured by two manufacturers and prepared by the authors, were tested for antigenicity, observed for appearance, and determined for specificity, ABH antigenicity, affinity, haemolysis rate and stability. Thirty-five plasma samples positive for irregular IgMs were determined by the prepared reagents, and the detection rates were compared. Results: All the blood cells of group O prepared by the authors showed reaction results of more than ++ with anti-P, anti-M, anti-N, anti-C, anti-c, anti-E and anti-e reagents. However, partial antibodies failed to be tested by the inverse ABO blood grouping reagents provided by two manufacturers. All the appearances, specificities, ABH anti-genicities, affinities, haemolysis rates and stabilities of the reagents met the relevant requirements and showed no significant difference. All the irregular IgMs were detected by the prepared reagent, partial of which failed to be detected by the reagents provided by two manufacturers. Conclusion: The method and standard for quality control of inverse ABO blood grouping established by the authors were suitable for detection of irregular IgMs.

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