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Hengshui, China

Sun J.-X.,Heng shui College | Wei L.-Q.,Heng shui College | Zhu H.-X.,Heng shui College
Modern Food Science and Technology

The ergosterol content in fermentation of Ganoderma lucidum was determined by HPLC at 282 nm. The results showed that the maximum standard deviation was 0.92% and the linear regression equation y=28685x-382.61 (R=0.9998) in the range of 20-500 μg. The precision test showed that Ganoderma lucidum extracellular and intracellular ergosterol content of RSD were 0.40% and 1.96%, respectively. The spike recoveries for ergosterol were between 96.82 and 102.50%. The method was feasibility and accurate for determination of ergosterol content in fermentation of Ganoderma lucidum. The ergosterol content of Ganoderma lucidum extracellular and intracellular were 0.07 mg/mL and 0.64 mg/g, respectively, after fermentation for 120 h. Source

Sun J.,Tianjin University of Science and Technology | Sun J.,Heng shui College | Zhu H.X.,Tianjin University of Science and Technology | Zhu H.X.,Heng shui College | And 2 more authors.
African Journal of Biotechnology

In this paper, the bacteriostatic action of purified raspberry flavonoid was explored. A study was conducted on the effect of raspberry flavonoid on Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Saccharomyces cerevisiae, Rhizopus, Mucor and Penicillium. Raspberry flavonoid had the best inhibition effect on S. aureus, followed by B. subtilis, E. coli and Penicillium. However, it cannot inhibit the growth of S. cerevisiae, Rhizopus and Mucor. The minimal inhibitory concentration was 0.16 mg/ml for E. coli, 0.08 mg/ml for B. subtilis, 0.04 mg/ml for S. aureus and 0.64 mg/ml for Penicillium. At 100°C, temperature does not have an obvious effect on the bacteriostatic action of raspberry flavonoid. The rate of inhibition increases with time and the increase of concentration of raspberry flavonoid. Raspberry flavonoid has a stable bacteriostatic action pH from 4 to 8. © 2012 Academic Journals. Source

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