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Cai X.-H.,Xinxiang Medical University | Cai X.-H.,Henan Universities | Zhao G.-A.,Xinxiang Medical University | Wang L.,Xinxiang Medical University | And 2 more authors.
Acta Anatomica Sinica | Year: 2012

Objective: To probe the change of myocardial cells and collagen I/III protein expression of the left ventricular induced by isoprenaline hydrochloride (ISO) in rats. Methods: Adult rats were treated with intraperitoneal injection of ISO by 4mg/(kg · d) for 1, 4 and 8 weeks to prepare SD rats model of myocardial ischemia. The control group were treated with the same volume of the physiologic saline. The tissue structure of the left ventricular was observed with the electron and light microscopes. The expression of collagen I/III protein was detected with immunohistochemistry and Western blotting. The expressive intension and the area percentage were statistically treated with the HAPIS-2000 image analysis system and SPSS10.0 software. Results: Under the light microscope, the necrotic area of myocardial ischemia appeared near the endocardium when induced by ISO. In the necrotic area of ischemia collagen I/III was of high expression, and the percentage of the necrotic area was of the decreasing tendency. The difference between groups of 1 week and 4 weeks was of no statistical significance (P > 0.05), and the difference between the groups of 4 weeks and 8 weeks was of statistical significance (P < 0.05). The difference of the expression intensity when compared among groups was of no statistical significance (P > 0.05). The early period of myocardial cells in the non-necrotic area of ischemia was the mass collection of glycogenosome, and the characteristic of the later period was the increase of the mitochondria and the loose myofibrilla. There was no obvious change in the area percentage of collagen I protein when detected with immunochemistry, and the difference among groups was of no statistical difference (P > 0.05). The difference of the expression intensity when compared among the experimental groups was of no statistical significance (P > 0.05), but compared with the control group, the expression intensity decreased and the difference was of statistical difference (P < 0.05). The area percentage of collagen III protein was of the increasing tendency when detected with immunochemistry, and the difference between the groups of 1 week and 4 weeks was of statistical significance (P < 0.05). The expression intensity was stable and the difference among groups was of no statistical significance (P < 0.05). The result of Western blotting showed that the collagen I/III protein among the experimental groups was of a tendency from increasing to decreasing, and the difference among the groups was of statistical significance (P < 0.05). Conclusion: Long-time inducement may lead to the ischemic necrosis of the myocardial cells near the endocardium. The necrotic area of ischemia is generally of a decreasing tendency and fibrous change appears in this area, which is associated with the increase of stiffness of the left ventricular wall. The hypertrophy of the myocardial cells in the non-necrotic area of ischemiais associated with the energy metabolism. Collagen I/III protein does not involve in the stiffness and the compliance of left ventricular wall. Source


Tian X.-Q.,Henan Universities | Wang L.,Henan Universities | Guo Z.-K.,Henan Universities | Cai X.-H.,Henan Universities | And 2 more authors.
Acta Anatomica Sinica | Year: 2012

Objective: To explore the network remodeling of collagen I and III protein in the heart of renal hypertension rats. Methods: The animal model was prepared by bilateral renal artery stenosis. Thirty-two SD rats were randomly divided into sham operation group, two weeks group, five weeks group and seven weeks group after operation. The model quality of hypertensive rats was assessed with noninvasive blood pressure analysis. The variation characteristics of collagen I and III in left ventricular wall were detected by immunohistochemistry and Western blotting, and the quantitive analysis was performed with the microscopic image analysis system. Results: The collagen I and III networks had no noticeable change at the second week after operation, but at the fifth week after operation, the thick fibers of collagen I around cardiomyocyte bundle were seen markedly broken and gathered together, or absent. The broken or accumulated fibers with irregular arrangement in collagen I sheath in tunica adventitia of arteriole were also detected. The thick and thin fiber bundles of the collagen III around cardimyocyte became loose and disorder at the fifth week after operation, and the collagen III sheath of tunica adventitia of arteriole turned to be loose. The results of microscopic image analysis showed that the difference of the mean optical density value and the average area ratio of collagen I had both significance between the fifth week and the seventh week after operation (P < 0.05). The difference of mean optical density value of collagen III had no significance between groups (P < 0.05), and the difference of the average area ratio had significance between the second week and the fifth week group after operation (P < 0.05). The results of Western boltting showed that the expression level of collagen I was gradually increased, and collagen III was gradually decreased from the fifth week after operation. Conclusion: The hypertension may cause severe damage to collagen I and III network of the left ventricular wall and the tunica adventitia of the vessels. Source


Li X.,Henan Universities | Sun S.,Henan Universities | Yang F.,Henan Universities | Kang J.,Henan Universities | And 3 more authors.
Organic and Biomolecular Chemistry | Year: 2015

An efficient and generally applicable protocol for palladium-catalyzed oxidative deacetonative coupling of 4-aryl-2-methyl-3-butyn-2-ols with dialkyl H-phosphonates has been developed. This methodology provides a new and practical route to alkynylphosphonates using the inexpensive 4-aryl-2-methyl-3-butyn-2-ols as the alkyne sources. This reaction could also be performed with aryl bromides, 2-methyl-3-butyne-2-ol and dialkyl H-phosphonates using the cheap 2-methyl-3-butyne-2-ol as an alkyne source. This journal is © The Royal Society of Chemistry 2015. Source

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