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Xu J.-L.,Henan Provincial Peoples Hospital | Li Y.-L.,Henan Provincial Peoples Hospital | Lian J.-M.,Henan Provincial Peoples Hospital | Dou S.-W.,Henan Provincial Peoples Hospital | And 3 more authors.
Neuroradiology | Year: 2010

Introduction: This study aims to evaluate the differentiated effectiveness of MR diffusion tensor imaging (DTI) to postoperative recurrent glioma and radiation injury. Methods: Conventional MRI and DTI examination were performed using Siemens 3.0 T MR System for patients with new contrast-enhancing lesions at the site of treated tumor with postoperative radiotherapy. The region of interest was manually drawn on ADC and FA maps at contrast-enhancing lesion area, peri-lesion edema, and the contra-lateral normal white matter. Then ADC and FA values were measured and, the ADC ratio and FA ratio were calculated. Twenty patients with recurrent tumor and 15 with radiation injury were confirmed by histopathologic examination (23 patients) and clinical imaging follow-up (12 patients), respectively. The mean ADC ratio and FA ratio were compared between the two lesion types. Results: The mean ADC ratio at contrast-enhancing lesion area was significantly lower in patients with recurrent tumor (1.34∈±∈0.15) compared to that with radiation injury (1.62∈±∈0.17; P∈<∈0.01). The mean FA ratio at contrast-enhancing lesion area was significantly higher in patients with recurrent tumor (0.45∈±∈0.03) compared to that with radiation injury (0.32∈±∈0.03; P∈<∈0.01). Neither mean ADC ratio nor FA ratio in edema areas had statistical difference between the two groups. A recurrent tumor was suggested when either ADC ratio∈<1.65 or/and FA ratio >0.36 at contrast-enhancing lesion area according to the receiver operating characteristics curve analysis. Three patients with recurrent tumor and two with radiation injury were misclassified. Conclusion: DTI is a valuable method to distinguish postoperative recurrent glioma and radiation injury. © 2010 Springer-Verlag.


Han L.,Henan Provincial Tumor Hospital
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2011

This study was purposed to explore the effect of hyperthermia on sensitivity of multiple myeloma cells RPMI 8226 to adriamycin (ADM) and its mechanism. The working concentration of ADM against RPMI 8226 cells was defined by MTT assay. RPMI 8226 cells were divided into 4 groups: control group, hyperthermia (42°C) group, chemotherapy (ADM) group and combination group (42°C + ADM), the survival rate of RPMI 8226 cells in 4 groups was detected by trypan blue exclusion, the inhibitory effect of hyperthermia on proliferation of RPMI 8226 cells was detected by MTT assay, the cell cycle distribution, apoptosis rate of cells, intracellular ADM concentration and P-gp expression level were measured by flow cytometry. The 1/4 IC(50) of ADM was defined as the working concentration in the experiment. The results indicated that the hyperthermia promoted the entering the cells from in G(0)/G(1) phase into S and G(2)/M phases, the expression of P-gp protein on cells in hyperthermia and combination groups was down-regulated, the intracellular ADM concentration in combination group obviously increased. It is concluded that the hyperthermia combined with ADM obviously enhance the inhibitory effect on proliferation of RPMI 8226 cells. The hyperthermia increases the sensitivity of RPMI 8226 cells to chemotherapy through down-regulating the expression of P-gp protein on cells and increasing the intracellular ADM concentration.


Jiang S.-Q.,Henan TCM College | Sun H.-X.,Henan Provincial TCM Hospital | Xu Y.-M.,Henan TCM College | Jiang Y.-L.,Henan Provincial TCM Hospital | And 2 more authors.
Journal of Traditional Chinese Medicine | Year: 2011

Objective: To observe the therapeutic effects of Jingyuankang capsules for leukopenia in AIDS patients. Methods: In this randomized double-blind trial, 58 patients orally took Jingyuankang capsule, analog Leucogen tablet and the HAART (highly active anti-retroviral therapy) drugs, and the other 58 patients took Leucogen tablet, analog Jingyuankang capsule and the HAART drugs all for 6 months, during which the peripheral hemogram was periodically examined to observe the therapeutic effects of Jingyuankang capsule for leukopenia of the AIDS patients. Results: With good therapeutic effect for leukopenia of the AIDS patients, Jingyuankang capsule can enhance leukocyte level as effective as Leucogen tablet in treating grade I and grade II leukopenia, and more effectively than Leucogen tablet in treating grade III leukopenia. No toxic side-effects and adverse reactions were found during the treatment and in the follow-up visit. Conclusion: Jingyuankang capsule can effectively treat leukopenia of the AIDS patients.


Zhao Y.Z.,Henan Provincial Tumor Hospital
Zhonghua yi xue za zhi | Year: 2011

To explore the effects of splenic flexure and sigmoid colon variation on anastomosis after left colectomy. The clinical data of 76 descending colon patients were collected retrospectively from March 2004 to April 2011 at our hospital. Statistical analysis was performed for the types of splenic flexure and sigmoid colon with regards to the choice of anastomosis. There were mesenteric type (n = 55), mobile type (n = 7) and adhesive type (n = 14) for splenic flexure. And among 61 regular types, 15 were of variable type for sigmoid colon variation. There was significant difference of anastomosis between the types of sigmoid colon variation [43 (78.2%) vs 5 (71.4%) vs 9 (64.3%), P > 0.05] while no significant difference existed between the types of splenic flexure [I type 56(91.8%) vs II type 1 (14.3%), III or IV type 0, P < 0.05]. A clinician should pay more attention to the types of sigmoid colon variation. And it helps to select the right approach of anastomosis after left colectomy.


Zhang J.,Henan Provincial Tumor Hospital | Lu J.,Email
Zhonghua zhong liu za zhi [Chinese journal of oncology] | Year: 2014

To summarize the results of endoscopic screening of esophageal, gastric cardiac and gastric cancers in the high-risk population, and analyze the influencing factors such as age, gender and biopsy rate on their detection and early diagnosis rates. Nine high incidence cities and counties of esophageal cancer in Henan province were included in this study. People aged 40-69 years were set to the target population. Excluding contraindications for gastroscopy, in accordance with the national technical scheme of early cancer diagnosis and treatment, gastroscopic screening and biopsy pathology for human esophageal, cardiac and gastric cancers were carried out. During the 3-year period, a total of 40 156 subjects were screened. Among them, 18 459 cases of various precancerous lesions (46.0%) were detected. The cancer detection rate was 2.3% (916 cases), including 763 cases of early cancers. The diagnosis rate of early cancers was 83.3%. Precancerous lesions were detected in 9297 cases (23.2%) for esophagus and 9162 cases (22.8%) for gastric cardia as well as stomach, respectively. The results of this study demonstrate that endoscopic screening is feasible for early detection, diagnosis and treatment of esophageal, gastric cardia and gastric cancers among high risk population in high incidence area. Exploration analysis of relevant affecting factors may help to further improve the screening project for early diagnosis and treatment of those cancers.


Xu J.-L.,Henan Provincial Peoples Hospital | Li Y.-L.,Henan Provincial Peoples Hospital | Lian J.-M.,Henan Provincial Peoples Hospital | Dou S.-W.,Henan Provincial Peoples Hospital | And 2 more authors.
Chinese Journal of Medical Imaging Technology | Year: 2010

Objective: To evaluate the differentiated effectiveness of two-dimensional proton MR spectroscopy (2D 1H-MRS) to post-operative recurrent glioma and radiation injury. Methods: Conventional MR and 2D 1H-MRS examinations were performed with Siemens 3.0T MR system for patients with recurrent contrast-enhancing lesions at the site of the treated glioma. The metabolite peaks were measured at the regions of enhanced nodule and edema, including N-acetylaspartate (NAA), choline-containing compounds (Cho) and creatine (Cr), while the Cho/Cr, Cho/NAA, NAA/Cr ratios were calculated. The new lesions were proved with histo-pathologic examination in 23 patients and clinical-imaging following-up in 12 patients. Recurrent gliomas were found in 20 patients, and radiation injury in 15 patients. Each mean metabolite ratio above was compared between two lesion types. Results: At contrast-enhancing regions and edema regions, the mean Cho/Cr and Cho/NAA ratios were all significantly higher in patients with tumor recurrence compared with those with radiation injury. Tthe mean NAA/Cr ratio was significantly lower in patients with tumor recurrence compared with that with radiation injury at contrast-enhancing regions, but no significant difference was found at edema regions. Taking Cho/Cr and (or) Cho/NAA ratios >1.77 based on ROC curves of metabolite ratios as standards, the diagnostic sensitivity, specificity and accuracy was 90.00% (18/20), 93.33% (14/15) and 91.43% (32/35), respectively. Conclusion: 2D 1H-MRS is a valuable method to distinguish postoperative recurrent glioma and radiation injury.


Yang H.,Henan Provincial Tumor Hospital | Liu H.,Fudan University | Peng W.,Fudan University | Hua Y.,Fudan University
Journal of Computer Assisted Tomography | Year: 2012

OBJECTIVE: To evaluate the role of magnetic resonance imaging (MRI) in the detection of residual disease for patients after excisional biopsy of breast carcinoma, before re-excision. MATERIALS AND METHODS: We performed a retrospective review of 97 patients who underwent MRI soon after excisional biopsy of breast carcinoma with undeterminable margin status before further surgical intervention to determine the value of MRI in detecting residual disease. RESULTS: The positive predictive value was 50.0%. The negative predictive value was 78.9%. The MRI detected 54.5% of the residual disease for invasive ductal carcinoma and 100% for ductal carcinoma in situ. Positive predictive value and sensitivity for MRI conducted within 14 days of the original surgery were lower than those for MRI conducted after 14 days of the original surgery, being 31.6% and 46.2% versus 66.7% and 73.4%, respectively. The difference between the positive predictive value within 14 days versus after 14 days was statistically significant. CONCLUSION: Magnetic resonance imaging in detecting residual disease soon after excisional biopsy for breast carcinoma had a low positive predictive value and moderately high negative predictive value. Magnetic resonance imaging was more sensitive in detecting residual ductal carcinoma in situ than invasive ductal carcinoma. Magnetic resonance imaging conducted more than 14 days after the original biopsy had a significantly higher positive predictive value than MRI done within 14 days. © 2012 Lippincott Williams & Wilkins, Inc.


Li H.,Peking Union Medical College | Gao Q.,Peking Union Medical College | Gao Q.,Henan Provincial Tumor Hospital | Guo L.,Peking Union Medical College | Lu S.H.,Peking Union Medical College
Cancer Biology and Therapy | Year: 2011

Recent reports have shown that cancer stem cells exist in many malignancies. Side population (SP ) cells are used to enrich cancer stem-like cells in many cell lines and fresh tumor specimens. In this study, we cultured primary esophageal squamous cell carcinoma (ES CC) cells from ES CC tissue specimens. SP cells from primary ES CC cells were more resistant to chemotherapeutic reagents and formed more colonies in vitro than non-SP cells. In addition, xenograft experiments revealed that SP cells were more tumorigenic in vivo. Further results indicated that the PI3K/Akt pathway is essential to SP cells through the regulation of ABCG2 transporter function. Furthermore, PTEN, rather than mTOR, was found to be involved in SP cell regulation in primary ES CC cells. These findings reveal that SP cells are enriched for cancer stem-like cells in primary ES CC cells and that the PTEN/PI3K/Akt pathway regulates this stem-like population. This study indicates that SP cells in primary culture cells from tissue specimens could be a promising model for cancer stem cell research and may help researchers develop novel therapeutic strategies or efficient drugs that target ESCC stem-like cells. © 2011 Landes Bioscience.


PubMed | Henan Provincial Tumor Hospital
Type: Journal Article | Journal: Journal of computer assisted tomography | Year: 2012

To evaluate the role of magnetic resonance imaging (MRI) in the detection of residual disease for patients after excisional biopsy of breast carcinoma, before re-excision.We performed a retrospective review of 97 patients who underwent MRI soon after excisional biopsy of breast carcinoma with undeterminable margin status before further surgical intervention to determine the value of MRI in detecting residual disease.The positive predictive value was 50.0%. The negative predictive value was 78.9%. The MRI detected 54.5% of the residual disease for invasive ductal carcinoma and 100% for ductal carcinoma in situ. Positive predictive value and sensitivity for MRI conducted within 14 days of the original surgery were lower than those for MRI conducted after 14 days of the original surgery, being 31.6% and 46.2% versus 66.7% and 73.4%, respectively. The difference between the positive predictive value within 14 days versus after 14 days was statistically significant.Magnetic resonance imaging in detecting residual disease soon after excisional biopsy for breast carcinoma had a low positive predictive value and moderately high negative predictive value. Magnetic resonance imaging was more sensitive in detecting residual ductal carcinoma in situ than invasive ductal carcinoma. Magnetic resonance imaging conducted more than 14 days after the original biopsy had a significantly higher positive predictive value than MRI done within 14 days.


PubMed | Henan Provincial Tumor Hospital
Type: Journal Article | Journal: Zhongguo shi yan xue ye xue za zhi | Year: 2011

This study was purposed to explore the effect of hyperthermia on sensitivity of multiple myeloma cells RPMI 8226 to adriamycin (ADM) and its mechanism. The working concentration of ADM against RPMI 8226 cells was defined by MTT assay. RPMI 8226 cells were divided into 4 groups: control group, hyperthermia (42C) group, chemotherapy (ADM) group and combination group (42C + ADM), the survival rate of RPMI 8226 cells in 4 groups was detected by trypan blue exclusion, the inhibitory effect of hyperthermia on proliferation of RPMI 8226 cells was detected by MTT assay, the cell cycle distribution, apoptosis rate of cells, intracellular ADM concentration and P-gp expression level were measured by flow cytometry. The 1/4 IC(50) of ADM was defined as the working concentration in the experiment. The results indicated that the hyperthermia promoted the entering the cells from in G(0)/G(1) phase into S and G(2)/M phases, the expression of P-gp protein on cells in hyperthermia and combination groups was down-regulated, the intracellular ADM concentration in combination group obviously increased. It is concluded that the hyperthermia combined with ADM obviously enhance the inhibitory effect on proliferation of RPMI 8226 cells. The hyperthermia increases the sensitivity of RPMI 8226 cells to chemotherapy through down-regulating the expression of P-gp protein on cells and increasing the intracellular ADM concentration.

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