Henan Provincial Academy of Agricultural science

Xinyang, China

Henan Provincial Academy of Agricultural science

Xinyang, China
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Wang J.,South China Agricultural University | Huang S.,South China Agricultural University | Li C.,South China Agricultural University | Ding W.,South China Agricultural University | And 2 more authors.
Chemistry of Natural Compounds | Year: 2015

A new coumarin, 7-(γ,γ-dimethylallyloxy)-6-hydroxy-4-methylcoumarin (1), was isolated from the mixed fermentation broth of two mangrove fungi (strain No. K38 and E33) isolated from the South China Sea coast. The structure of 1 was determined by comprehensive spectroscopic methods, especially 2D NMR techniques. Primary bioassays showed that compound 1 at 0.25 mM has no inhibitory activity against Fusarium graminearum, Gloeosporium musae, Rhizoctonia solani, and Phytophthora sojae. © 2015 Springer Science+Business Media New York

Zhang G.,Northwest University, China | Zhang G.,Shaanxi University of Chinese Medicine | Li Y.-M.,Northwest University, China | Li Y.-M.,Shaanxi University of Chinese Medicine | And 10 more authors.
Biologia Plantarum | Year: 2011

A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and designated as TaHIR2. The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) were 31. 05 kD and 5. 18, respectively. Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein. Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2. Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells. Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage. These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust. © 2011 Springer Science+Business Media B.V.

Sang S.,Nanjing Agricultural University | Sang S.,Henan Provincial Academy of Agricultural science | Li X.,Nanjing Agricultural University | Gao R.,Nanjing Agricultural University | And 6 more authors.
Plant Molecular Biology | Year: 2012

Harpin proteins secreted by phytopathogenic bacteria have been shown to activate the plant defense pathway, which involves transduction of a hydrogen peroxide (H 2O 2) signal generated in the apoplast. However, the way in which harpins are recognized in the pathway and what role the apoplastic H 2O 2 plays in plant defenses are unclear. Here, we examine whether the cellular localization of Hpa1 Xoo, a harpin protein produced by the rice bacterial leaf blight pathogen, impacts H 2O 2 production and pathogen resistance in Arabidopsis thaliana. Transformation with the hpa1 Xoo gene and hpa1 Xoo fused to an apoplastic localization signal (shpa1 Xoo) generated hpa1 Xoo- and shpa1 Xoo-expressing transgenic A. thaliana (HETAt and SHETAt) plants, respectively. Hpa1 Xoo was associated with the apoplast in SHETAt plants but localized inside the cell in HETAt plants. In addition, Hpa1 Xoo localization accompanied H 2O 2 accumulation in both the apoplast and cytoplasm of SHETAt plants but only in the cytoplasm of HETAt plants. Apoplastic H 2O 2 production via nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) located in the plasma membrane is a common feature of plant defenses. In SHETAt plants, H 2O 2 was generated in apoplasts in a NOX-dependent manner but accumulated to a greater extent in the cytoplasm than in the apoplast. After being applied to the wild-type plant, Hpa1 Xoo localized to apoplasts and stimulated H 2O 2 production as in SHETAt plants. In both plants, inhibiting apoplastic H 2O 2 generation abrogated both cytoplasmic H 2O 2 accumulation and plant resistance to bacterial pathogens. These results suggest the possibility that the apoplastic H 2O 2 is subject to a cytoplasmic translocation for participation in the pathogen defense. © 2012 Springer Science+Business Media B.V.

Li X.,Nanjing Agricultural University | Li X.,Henan Provincial Academy of Agricultural science | Han B.,Nanjing Agricultural University | Xu M.,Nanjing Agricultural University | And 5 more authors.
Planta | Year: 2014

The harpin protein Hpa1 produced by the bacterial blight pathogen of rice induces several growth-promoting responses in plants, activating the ethylene signaling pathway, increasing photosynthesis rates and EXPANSIN (EXP) gene expression levels, and thereby enhancing the vegetative growth. This study was attempted to analyze any mechanistic connections among the above and the role of gibberellin in these responses. Hpa1-induced growth enhancement was evaluated in Arabidopsis, tomato, and rice. And growth-promoting responses were determined mainly as an increase of chlorophyll a/b ratio, which indicates a potential elevation of photosynthesis rates, and enhancements of photosynthesis and EXP expression in the three plant species. In Arabidopsis, Hpa1-induced growth-promoting responses were partially compromised by a defect in ethylene perception or gibberellin biosynthesis. In tomato and rice, compromises of Hpa1-induced growth-promoting responses were caused by a pharmacological treatment with an ethylene perception inhibitor or a gibberellin biosynthesis inhibitor. In the three plant species, moreover, Hpa1-induced growth-promoting responses were significantly impaired, but not totally eliminated, by abolishing ethylene perception or gibberellin synthesis. However, simultaneous nullifications in both ethylene perception and gibberellin biosynthesis almost canceled the full effects of Hpa1 on plant growth, photosynthesis, and EXP2 expression. Theses results suggest that ethylene and gibberellin coregulate Hpa1-induced plant growth enhancement and associated physiological and molecular responses. © 2014 The Author(s).

Zhang G.,Northwest University, China | Zhang G.,Shaanxi University of Chinese Medicine | Sun Y.F.,Northwest University, China | Sun Y.F.,Shihezi University | And 11 more authors.
Biologia Plantarum | Year: 2013

Plant C2 domain proteins play important roles in diverse cellular processes including growth, development, and membrane targeting, as well as in abiotic and biotic stress adaptations by sensing intracellular Ca2+ signals. In this study, we isolated a novel C2 domain protein gene, TaERG3, from wheat infected by Puccinia striiformis f. sp. tritici. TaERG3 was predicted to encode a 144 amino acid protein with molecular mass of 15.68 kD and isoelectric point of 3.93. Analysis of the deduced amino acid sequence of TaERG3 using InterProScan revealed the presence of an N-terminal calciumdependent phospholipid-binding module (C2 domain, 5 to 103). Transient expression analysis showed that the TaERG3 protein was predominately and uniformly localized in the plasmalemma and nucleus of onion epidermal cells. Quantitative real-time PCR analyses indicated that TaERG3 transcript was differentially induced in both incompatible and compatible interactions, as well as by applied abscisic acid (ABA) and CaCl2. However, the significant transcript changes induced by methyl jasmonate, ethylene, and salicylic acid treatments were not as dramatic as those induced by ABA. TaERG3 was also up-regulated by environmental stimuli including low temperature and high salinity. These results imply that TaERG3 might be involved in wheat defence responses against stripe rust and abiotic stresses in an ABA-dependent signalling pathway. © 2013 Springer Science+Business Media Dordrecht.

PubMed | South China Agricultural University, University of Mississippi and Henan Provincial academy of Agricultural science
Type: Journal Article | Journal: Pharmacognosy magazine | Year: 2014

Myoporum bontioides A. Gray, an evergreen shrub from the Myoporaceae family, is a commonly used medicinal plant. Many studies have been conducted on the biologically active constituents of whole parts of M. bontioides. However, the endophytes of M. bontioides have not been intensively investigated. A new chlorine-containing isocoumarin, named dichlorodiaportinol A (1) was isolated from the endophytic fungus Trichoderma sp. 09 isolated from the root of M. bontioides. Its cytotoxic activity against human breast cancer (MCF-7) and human liver cancer (HepG2) cell lines was evaluated.Different open silica gel column chromatographic techniques with different solvent systems were used for the separation of the constituents of the ethyl acetate extract of the culture broth of the endophytic fungus Trichoderma sp. 09. The structure of compound one was identified by analysis of spectroscopic data [one-dimensional (1D), two-dimensional (2D)-nuclear magnetic resonance (NMR), ultraviolet (UV), infrared (IR) and Mass spectrometry (MS)]. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay method was used for the evaluation of cytotoxic activity of compound one against MCF-7 and HepG2 cell lines.Compound one was identified as 3-(3,3-dichloro-2,3-dihydroxy-propyl)-8-hydroxy-6- methoxy-isochromen-1-one. It inhibited MCF-7 and HepG2 cell lines, with half maximal inhibitory concentration (IC50) values of 17.8 and 39.6 g/mL, respectively.Compound one is a new chlorine-containing isocoumarin with moderate cytotoxic activity against MCF-7 and HepG2 cell lines. Thus, endophytes of M. bontioides are worthy of consideration for the development and research of antitumor agents.

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