Zhan S.-H.,Henan Province Armed Police Hospital |
Zhang L.-F.,Henan Province Armed Police Hospital |
Yao W.-M.,Henan Province Armed Police Hospital |
Yang M.-H.,Henan Province Armed Police Hospital |
And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2013
BACKGROUND: Studies have shown that cord blood mesenchymal stem cells can be induced into hepatocyte-like cells in a specific environment, and the transplanted cells can exercise the normal function of liver cells. OBJECTIVE: To establish the rat model of hepatic cirrhosis induced with carbon tetrachloride, and to observe the therapeutic effect of umbilical cord blood mesenchymal stem cell transplantation on a rat model of hepatic cirrhosis. METHODS: Hepatic cirrhosis models were established through carbon tetrachloride inducing, then 20 rat models of hepatic cirrhosis were randomly divided into two groups. The control group was injected with 0.5 mL normal saline via the tail vein; the treatment group was injected with chloromethyl benzamide labeled umbilical cord blood mesenchymal stem cell suspension, and the number of the stem cells was 1×106. After 2 weeks, all animals were sacrificed. Liver tissues were obtained for the histological observation and the venous blood was collected to detected the liver function indicators. RESULTS AND CONCLUSION: The liver cells of the hepatic cirrhosis models were loose and cloudy swelling, part of the cells were degenerated and necrosis, the structure of the hepatic lobule was vague, and several pseudolobules with different sizes were formed which meet the diagnostic criteria of hepatic cirrhosis. Compared with the control group, the level of serum albumin in the treatment group was significantly increased (P < 0.05), while the levels of bilirubin and transaminases in the treatment group were significantly decreased (P < 0.05). There were lots of red chloromethyl benzamide labeled umbilical cord blood mesenchymal stem cells in the liver tissue of the treatment group. Human umbilical cord blood mesenchymal stem cells can effectively improve the physiological function of hepatic cirrhosis through tail vein.