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Ning C.-J.,Henan Agricultural University | Ning C.-J.,Shanxi Agricultural University | Ning C.-J.,Henan Key Laboratory of Fruit and Cucurbit Biology | Zhu C.,Henan Agricultural University | And 11 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2012

Combined forms, distribution of selenium in apple leaves, extraction and separation technologies of macromolecule containing selenium were studied through the selecting of best concentration of selenium for foliar application on 'Red Fuji' apple. The results showed that: (1) Selenium of 100 mg · L-1 was the optimal choice for 'Red Fuji' apple trees. Treated with this concentration, the total selenium content in apple leaves was 2.7283 mg · kg-1 (FW), including organic selenium 2.3384 mg · kg-11 (FW). Organic degree was 85.71%. (2) Organic selenium mainly existed as protein combined form, which accounted for 77.09% of total organic selenium, both in control group and treatment groups. (3) The combined percentages of selenium with protein, polysaccharide and nucleic acid were 77.42%, 18.46% and 0.54% respectively in apple leaves of optimum treatment 100 mg · L-1. Therefore, Se protein was the main combined form of selenium in apple leaves. (4) Among the protein components, salt-solubility protein combined the most selenium, accounting for protein selenium content 46.27% in optimum treatment 100 mg · L-1. Source

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