Key Laboratory for Animal derived Food Safety of Henan Province

Zhenzhou, China

Key Laboratory for Animal derived Food Safety of Henan Province

Zhenzhou, China
SEARCH FILTERS
Time filter
Source Type

Zhou Y.,Henan Agricultural University | Jin X.-H.,Henan Agricultural University | Jing Y.-X.,Key Laboratory for Animal derived Food Safety of Henan Province | Song Y.,Key Laboratory for Animal derived Food Safety of Henan Province | And 7 more authors.
Veterinary Microbiology | Year: 2017

Porcine parvovirus virus (PPV) is an animal virus that has caused high economic losses for the swine industry worldwide. Previous studies demonstrated that PPV infection induced significant production of interleukin 6 (IL-6) in vitro and in vivo. However, the inflammatory cytokines and specific signaling pathways induced during PPV infection remain largely unknown. In the present study, we analyzed the expression levels of IL-6 in PPV-infected porcine kidney 15 (PK-15) and the results showed that PPV infection induced the increase of IL-6 mRNA expression in a dose-dependent manner. We also detected the expression of toll-like receptor 9 (TLR9) signaling proteins in the mRNA expressing level, when compared with the control group, the TLR9 expression in mRNA level increased at 24 h in PK-15 cells after PPV infection and reached the peak level at 48 h. In addition, the transcript profile of nuclear factor kappa B (NF-κB) signal pathway relating genes (MyD88, IRAK1, TRAF6, TAK1α, IκBκB and NF-κB) expression levels increased at different times. Furthermore, to verify the IL-6 expression was specific with the TLR9 expression and then by activating the NF-κB signal pathway, TLR9 and NF-κB specific inhibitors were applied during PPV infection, separately, the result indicated that the expression of IL-6 was decreased after inhibitor treatment. Taken together, PPV infection significantly induced IL-6 expression and this induction depended on NF-κB activation and TLR9 signaling pathways in PK-15 cell. © 2017 Elsevier B.V.


Zheng L.-L.,Henan Agricultural University | Wang Y.-B.,Key Laboratory for Animal derived Food Safety of Henan Province | Li M.-F.,Henan Agricultural University | Chen H.-Y.,Key Laboratory for Animal derived Food Safety of Henan Province | And 5 more authors.
Journal of Virological Methods | Year: 2013

The development of a SYBR Green-based duplex real-time PCR is described for simultaneous detection of porcine parvovirus (PPV) and porcine circovirus type 2 (PCV-2) genomes. Viral genomes were identified in the same sample by their distinctive melting temperature (Tm) which is 77.5°C for PPV VP2 313bp amplicon and 82.3°C for PCV-2 ORF2 171bp amplicon, respectively. The detection limit of the method was 0.01TCID50/mL for PPV and PCV-2, about 10 times more sensitive than conventional PCR. In addition, PPV and PCV-2 viral load were measured in 126 field samples, confirming the sensitivity and specificity, and the result showed that 70/126 samples were positive for PPV and 92/126 samples were positive for PCV2 by the duplex real-time PCR. This method may be a useful alternative rapid and reliable method for the detection of PPV/PCV-2 co-infection. © 2012 Elsevier B.V.


Wang R.-N.,Henan Agricultural University | Wang Y.-B.,Henan Agricultural University | Geng J.-W.,Henan Agricultural University | Guo D.-H.,Henan Agricultural University | And 5 more authors.
Vaccine | Year: 2012

Inactivated porcine parvovirus (PPV) vaccines are available commercially and widely used in the breeding herds. However, inactivated PPV vaccines have deficiencies in induction of specific cellular immune response. Transfer factor (TF) is a material that obtained from the leukocytes, and is a novel immune-stimulatory reagent that as a modulator of the immune system. In this study, the immunogenicity of PPV oil emulsion vaccine and the immuno-regulatory activities of TF were investigated. The inactivated PPV oil emulsion vaccines with or without TF were inoculated into BALB/c mice by subcutaneous injection. Then humoral and cellular immune responses were evaluated by indirect enzyme-linked immunosorbent assays (ELISA), fluorescence-activated cell sorter analyses (FACS). The results showed that the PPV specific immune responses could be evoked in mice by inoculating with PPV oil emulsion vaccine alone or by co-inoculation with TF. The cellular immune response levels in the co-inoculation groups were higher than those groups receiving the PPV oil emulsion vaccine alone, with the phenomena of higher level of IFN-γ, a little IL-6 and a trace of IL-4 in serum, and a vigorous T-cell response. However, there was no significant difference in antibody titers between TF synergy inactivated vaccine and the inactivated vaccine group (P> 0.05). In conclusion, these results suggest that TF possess better cellular immune-enhancing capability and would be exploited into an effective immune-adjuvant for inactivated vaccines. © 2012 Elsevier Ltd.

Loading Key Laboratory for Animal derived Food Safety of Henan Province collaborators
Loading Key Laboratory for Animal derived Food Safety of Henan Province collaborators