Henan Entry Exit Inspection and Quarantine Bureau

Zhengzhou, China

Henan Entry Exit Inspection and Quarantine Bureau

Zhengzhou, China

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Zhang Y.Y.,University of Science and Technology of China | He C.X.,University of Science and Technology of China | Zhu M.M.,HeNan Entry Exit Inspection and Quarantine Bureau
Advanced Materials Research | Year: 2013

In this paper Flexible flywheel EP6's several working conditions had be Simulation, Under a certain Factor of safety conditions. The reasonable Structure design had be found, through Compared with the results of fatigue test. Therefore, the Objective had be Achieved of improve design, reduce the times of experiment and shorten the cycle of research. © (2013) Trans Tech Publications, Switzerland.


Zhang Y.Y.,University of Science and Technology of China | Zhu M.M.,Henan Entry Exit Inspection and Quarantine Bureau
Advanced Materials Research | Year: 2011

Based on the large analysis software ANSYS, the FEA model of feeder was be setted up, the mechanical properties of the material was considered ,and the stress and deformation under loaded of two type materials HDPE and LDPE were be Comparative analysied, Results show that: the funnel's stress smaller,the maximum stress only 2-3Mpa and no more than 7-31Mpa of two materials' tensile strength.The funnel's deformation in allowing strain range about 2-3 mm, so structure is safe.In addition,the feeder legs bearred bigger force about 60Mpa, because the material was used Q345 steel, so the strength was enough and HDPE material feeder have leeway of reduced materials. © 2011 Trans Tech Publications.


Zhu W.,Henan Entry Exit Inspection and Quarantine Bureau | Zhang L.,Henan Entry Exit Inspection and Quarantine Bureau | Li S.,Henan University | Liu Y.,Henan Entry Exit Inspection and Quarantine Bureau | Yang J.,Henan Entry Exit Inspection and Quarantine Bureau
Chinese Journal of Chromatography (Se Pu) | Year: 2016

A confirmative method was developed for determining β-methylphenethylamine (BMPEA) and picamilon in health products by ultra performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry (UPLC-Q Trap MS). The sample was ultrasonically extracted with methanol. The purification was carried out on a new reversed phase solid-phase extraction column (PRiME HLB). The detection of compounds was in the positive mode. Multiple technologies were applied to solve the identification and quantification for the two compounds, such as multiple reaction monitoring (MRM), online enhanced product ion full scan (EPI) and library search. Methanol and 0.1% (v/v) formic acid aqueous solution were used as the mobile phases with gradient elution. A SHISEIDO CAPCELL MG II C18 column was used to separate the analytes. The LODs and LOQs for BMPEA and picamilon in liquid, table and capsule samples were 0. 2-0.5 μg/kg and 0.5-2.0 μg/kg, respectively. The average spiked recoveries of the method varied from 79.5% to 103.8% and the RSDs were between 3.6% and 15.4%. In the range of 0.2-200.0 μg/kg, the peak areas and concentrations of matrix standard solution showed good linear relationship (r≥0.9969). The qualitative confirmation results were required based on the search-match MRM and EPI mass spectra simultaneously. The method is simple and accurate for the determination of BMPEA and picamilon in imported and commercial health products.


Zhu W.,Henan Entry Exit Inspection and Quarantine Bureau | Yang J.,Henan Entry Exit Inspection and Quarantine Bureau | Yuan P.,Henan Entry Exit Inspection and Quarantine Bureau | Sun Z.,Henan Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Se pu = Chinese journal of chromatography / Zhongguo hua xue hui | Year: 2013

An analytical method was developed for the determination of total residues of ribavirin and its phosphorylated metabolites in chicken and its products by hydrophilic interaction chromatography-tandem mass spectrometry. The samples were extracted with acetonitrile containing 1% (v/v) acetic acid under ultrasonic condition and then enzymatically hydrolysed with acid phosphatase at 37 degrees C. After liposoluble substances being removed with hexane, C18 and PSA dispersion solid phase extraction (DSPE) was introduced to cleanup procedures. The separation was performed on an ultra-performance hydrophilic interaction chromatographic (HILIC) amide column under a gradient elution using acetonitrile and 0.1% formic acid. The analytes were detected in the positive electrospray ionization and multi-reaction monitoring (MRM) mode. In the range of 1-200 microg/kg, the correlation coefficient was greater than 0.999. The limit of detection (LOD, S/N > or = 3) was 1 microg/kg and the limit of quantification (LOQ, S/N > or = 10) was 5 microg/kg. The recoveries of ribavirin spiked at three levels ranged from 67.8% to 112.7% with the relative standard deviations (RSDs) of 6.1%-13.6%. The results of the determination of ribavirin in various real samples showed that the method is simple, rapid, accurate and suitable for the determination of total residues of ribavirin and its metabolites in chicken and its products.


Zhang Y.Y.,University of Science and Technology of China | Zhu M.M.,Henan Entry Exit Inspection and Quarantine Bureau
Advanced Materials Research | Year: 2011

Asphalt need asphalt box to preservate heat in the production and use process,if stored appropriate, asphalt can repeated heating namely its performance won't be damaged badly under higer temperature keep a very long time,So, the asphalt box's performance is very important. in this paper, the mechanical properties of the material was considered, the FEA model of asphalt box was be setted up,the stress and strain of unloading entrance was be analysed when in the 4g longitudinal accelerated speed and a standpoint was be determined that the structure performance of asphalt box is Meets the related standard requirements. © 2011 Trans Tech Publications.


Zhu W.,Henan Entry Exit Inspection and Quarantine Bureau
Se pu = Chinese journal of chromatography / Zhongguo hua xue hui | Year: 2010

A quick confirmative method was developed for determining the residues of 17 glucocorticoids, 11 progesterones, 3 androgens and 8 estrogens in infant formula by liquid chromatography-tandem quadrupole mass spectrometry (LC-MS/MS). The sample was extracted with acetonitrile at first. Then the lipid substances were removed by centrifugation under freezing condition and liquid-liquid extraction with hexane of the extract. The purification was carried out on hydrophilic-lipophilic solid-phase extraction columns and methanol was used as the eluted solvent. The detection of 39 analytes was carried out in the positive or negative multi-reaction monitoring (MRM) mode, separately. Acetonitrile-0.1% formic acid was used as the mobile phase and an ordinary silica gel C18 column was selected to separate the analytes in the positive mode. Acetonitrile-0.1% aqueous ammonia as mobile phase and the separation was carried out on an ultra-performance C18 column with a wide pH range in the negative mode. The limits of quantification (LOQ, S/N > or = 10) were 0.02-5 microg/kg. The overall recoveries varied from 59.5% to 117.9%, and the relative standard deviations (RSD) were between 6.4% and 16.3%. The real sample tests showed that the simple and accurate method can be used for determining the residues of multi-endogenous and chemically synthesized hormones in milk powders.


Sun W.,Henan Entry Exit Inspection and Quarantine Bureau | Zhao B.,Henan Entry Exit Inspection and Quarantine Bureau | Zhang S.,Henan Entry Exit Inspection and Quarantine Bureau | Sun Z.,Henan Entry Exit Inspection and Quarantine Bureau | Zhang L.,Henan Entry Exit Inspection and Quarantine Bureau
Chinese Journal of Chromatography (Se Pu) | Year: 2012

A method was developed for the determination of the residue of phenylethanolamine A in feeds by ultra performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS) The phenylethanolamine A in sample was extracted with phosphoric acid methanol solution. The extract was purified and concentrated by an MCX column.In the positive mode, an ultra-performance BEH C18 column (100 mm × 2.1 mm, 1.7 μm) was used to separate the phenylethanolamine A, and the separation was carried out on a Waters UPLC system by using gradient elution with acetonitrile and 0.1% (v/v)formic acid aqueous solution as the mobile phases at a flow rate of 0.4 mL/min.The qualitative and quantitative analysis was carried out by multi-reaction monitoring (MRM) mode, in which the analyte was ionized by electrospray ionization interface (ESI). The results showed that, the calibration curve was linear in the range of 0.5-100 μg/kg (r>0.999), the limit of quantification (S/N>10) was 10 μg/kg, the recoveries ranged from 79.6% to 86.2%, and the relative standard deviations (RSDs) were between 3.1% and 6.7%. The real sample tests showed that the method is convenient and reliable.


Zhu W.,Henan Entry Exit Inspection and Quarantine Bureau | Liu Y.,Henan Entry Exit Inspection and Quarantine Bureau | Yuan P.,Henan Entry Exit Inspection and Quarantine Bureau | Yang J.,Henan Entry Exit Inspection and Quarantine Bureau
Chinese Journal of Chromatography (Se Pu) | Year: 2010

A quick confirmative method was developed for determining the residues of 17 glu-cocorticoids 11 progesterones 3 androgens and 8 estrogens in infant formula by liquid chromatography-tandem quadrupole mass spectrometry (LC-MS/MS). The sample was extracted with acetonitrile at first. Then the lipid substances were removed by centrifugation under freezing condition and liquid-liquid extraction with hexane of the extract. The purification was carried out on hydrophilic-lipophilic solid-phase extraction columns and methanol was used as the eluted solvent. The detection of 39 analytes was carried out in the positive or negative multi-reaction monitoring (MRM/ mode, separately. Acetonitrile-O. 1% formic acid was used as the mobile phase and an ordinary silica gel C18 column was selected to separate the analytes in the positive mode. Acetonitrile-O. 1% aqueous ammonia as mobile phase and the separation was carried out on an ultra-performance C18 column with a wide pH range in the negative mode. The limits of quantification (LOQ, S/N≥10 were 0.02-5 μg/kg. The overall recoveries varied from 59.5% to 117. 9%, and the relative standard deviations CRSD) were between 6. 4% and 16. 3%. The real sample tests showed that the simple and accurate method can be used for determining the residues of multi-endogenous and chemically synthesized hormones in milk powders.


PubMed | Henan Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Se pu = Chinese journal of chromatography | Year: 2010

A confirmative method was developed for the determination of four triorganotins (TOTs) included tributyltin (TBT), triphenyltin (TPhT), di-triphenyltin oxide (DTPhT), and cyhexatin (CYT) in leather products by cation solid-phase extraction (SPE) and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The organotin residues were extracted with HCl solution. The ionized compounds were purified with SPE. The reconstituted sample solution was analyzed by UPLC-MS/MS in positive ion electrospray and multi-reaction monitor (MRM) mode, with a UPLC C18 column as the separation column. The limits of quantification were 10 microg/kg for TBT, TPhT, DTPhT (calculated as TPhT) and 20 microg/kg for CYT. The method was validated at three levels for each compound. The recoveries were from 54.1% to 101.4%, and the relative standard deviations (RSD) were between 4.3% and 9.8%. The method is simple, reliable and accurate. It can meet the requirements of the domestic and international legislations. The method adapts to simultaneously confirm the residues of the four TOTs in leather products.


PubMed | South China Agricultural University, Guangdong Academy of Agricultural Sciences, Guangdong Dahuanong Animal Health Products Co. and Henan Entry Exit Inspection and Quarantine Bureau
Type: | Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases | Year: 2014

Complete genome characterization of porcine circovirus type 2 (PCV2) for bovid origins was still unclear in China. Therefore, in this study, PCV2 full-length genome of buffalo-origin was amplified and analyzed using PCR, DNAStar and MEGA 5.1. Genome size of three distinct PCV2 strains (buffalo1, buffalo2 and buffalo3) was 1767 bp (48.56% G+C), 1767 bp (48.67% G+C) and 1768 bp (48.08% G+C), respectively. At the nucleotide level, their identity varied from 95% to 96% for complete genome, from 97% to 97.8% for ORF1, and from 90.6% to 94.4% for ORF2. At the amino acid level, their identity varied from 98.7% to 99% for ORF1, and from 88% to 94.9% for ORF2. Online Blast analysis showed that buffalo1, buffalo2 and buffalo3 had highest nucleotide identity (varied from 99.77% to 99.83%) with porcine-origin PCV2 strains. Moreover, in the phylogenetic tree, they were divided into three different clusters and belonged to the worldwide accepted genotypes of PCV2b, PCV2c and PCV2a, respectively. To summarize, this study first recorded complete genome information of PCV2 for non-porcine origins in China.

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