Henan Academy of Medical science

Zhengzhou, China

Henan Academy of Medical science

Zhengzhou, China
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Wang G.,Henan University of Science and Technology | Zeng G.,Henan University of Science and Technology | Wang C.,Henan University of Science and Technology | Wang H.,People Hospital of Zhengzhou | And 4 more authors.
Biomedical Papers | Year: 2015

Background and Aim. Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling. Methods. Using 0.75, 1.5 and 3.0 μL between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers. Results. Quantum dot concentration 0.75 μg/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106. Conclusions. Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility. © 2015 PALACKY UNIV. All rights reserved.


PubMed | Zhengzhou University, Henan University of Science and Technology, People Hospital of Zhengzhou and Henan Academy of Medical science
Type: Journal Article | Journal: Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia | Year: 2015

Amniotic membrane-derived mesenchymal stem cells (hAM-dMSCs) are a potential source of mesenchymal stem cells which could be used to repair skin damage. The use of mesenchymal stem cells to repair skin damage requires safe, effective and biocompatible agents to evaluate the effectiveness of the result. Quantum dots (QDs) composed of CdSe/ZnS are semiconductor nanocrystals with broad excitation and narrow emission spectra, which have been considered as a new chemical and fluorescent substance for non-invasively labeling different cells in vitro and in vivo. This study investigated the cytotoxic effects of QDs on hAM-dMSCs at different times following labeling.Using 0.75, 1.5 and 3.0 L between quantum dots, labeled human amniotic mesenchymal stem cells were collected on days 1, 2 and 4 and observed morphological changes, performed an MTT cell growth assay and flow cytometry for mesenchymal stem cells molecular markers.Quantum dot concentration 0.75 g/mL labeled under a fluorescence microscope, cell morphology was observed, The MTT assay showed cells in the proliferative phase. Flow cytometry expression CD29, CD31, CD34, CD44, CD90, CD105 and CD106.Within a certain range of concentrations between quantum dots labeled human amniotic mesenchymal stem cells has good biocompatibility.


Jiang J.,Zhengzhou University | Jiang J.,Key Laboratory of Tumor Epidemiology of Henan Province | Deng S.,University of South Carolina | Chen Y.,Zhengzhou University | And 14 more authors.
International Journal of Cardiology | Year: 2016

Background The aim of the study was to compare the efficiency of bioelectrical indices (percentage body fat, PBF; visceral fat index, VFI) and various anthropometric measures (body mass index, BMI; waist circumference, WC; waist-to-height ratio, WHtR) on determining hypertension in Chinese. Methods We conducted the community-based cross-sectional survey during August of 2013 to August of 2015 in 66 sample sites selected by multistage random sampling method from Henan province. 14,364 residents were included in the study. Results In both genders, VFI and PBF tended to rise with age. However, for each age-specific group, men consistently had significantly greater VFI than women (all P < 0.0001) and women had considerably higher PBF (all P < 0.0001). The odds ratios and area under the ROC curves (AUCs) for hypertension associated with adiposity indices decreased with age. In younger (15 ∼ 34 year) men and women, VFI had the highest crude (2.43-7.95) and adjusted (2.40-11.63) odds ratio for hypertension. The AUCs for PBF, VFI and WHtR were significantly larger than those for BMI and WC (all P < 0.01). Whereas no statistically significant difference were found in AUCs among PBF, VFI and WHtR (all P > 0.10). Additionally, VFI and PBF yielded the greatest Youden index in identifying hypertension in men (0.27) and women (0.34), respectively. Optimal cutoffs for VFI/PBF were 11.70/24.45 and 7.55/33.65 in men and women, respectively. Conclusions VFI and PBF could be better candidates for identifying hypertension in men and women, respectively. Adolescents and young adults should be highlighted in preventing hypertension by control of excess body and visceral fat. © 2016 Elsevier Ireland Ltd.


Cheng T.,Zhengzhou University | Cheng T.,Johns Hopkins University | Yang B.,Zhengzhou University | Li D.,Zhengzhou University | And 8 more authors.
Cellular and Molecular Neurobiology | Year: 2015

Traumatic brain injury (TBI), which can lead to disability, dysfunction, and even death, is a prominent health problem worldwide. Effective therapy for this serious and debilitating condition is needed. Human umbilical cord matrix, known as Wharton’s jelly (WJ), provides a natural, interface scaffold that is enriched in mesenchymal stem cells. In this study, we tested the efficacy of WJ tissue transplantation in a weight-drop model of TBI in rats. WJ tissue was cultured and transplanted into the injury site 24 h after TBI. The modified neurologic severity score, body weight, brain edema, and lesion volume were evaluated at various time points after TBI. Cognitive behavior was assessed by the novel object recognition test and the Morris water maze test. Expression of brain-derived neurotrophic factor (BDNF) in the perilesional brain area was measured at day 14 after TBI. We found that WJ tissue transplantation lessened TBI-induced brain edema (day 3), reduced lesion volume (day 28), improved neurologic function (days 21–28), and promoted memory and cognitive recovery. Additionally, expression of BDNF mRNA and protein was higher in WJ tissue-treated rats than in sham-operated or vehicle-treated rats. These data suggest that WJ tissue transplantation can reduce TBI-induced brain injury and may have therapeutic potential for the treatment of TBI. © 2015, Springer Science+Business Media New York.


Zhao Y.,Zhengzhou University | Wang B.,Zhengzhou University | Hu K.,Henan Academy of Medical science | Wang J.,Zhengzhou University | And 5 more authors.
Oncology Letters | Year: 2015

The GSTT1 gene encodes a key enzyme involved in the metabolism of xenobiotics and its polymorphisms have been associated with individual susceptibility to various malignancies. Numerous molecular epidemiological studies have been performed to investigate the association between GSTT1 gene polymorphisms and lung cancer susceptibility; however, the results of previous studies were inconsistent. Therefore, the aim of the present study was to conduct a meta-analysis in order to derive a more precise estimation of the association in the East Asian populations. The meta-analysis included 7,415 lung cancer cases and 6,084 controls from 26 published studies in East Asia, which were selected from the PubMed and China National Knowledge Infrastructure databases, up to March 20, 2014. Using crude odds ratios (ORs) with 95% confidence intervals (CIs), a statistically significant association was identified between the GSTT1 null genotype and lung cancer in the East Asian populations (OR=1.17; 95% CI, 1.09-1.25; Pheterogeneity=0.003). Furthermore, subgroup analyses revealed that the lung cancer risk in smokers carrying the GSTT1 null genotype was significantly increased compared with non-smokers (OR=1.71; 95% CI, 1.04-2.81; Pheterogeneity=0.002). Thus, the GSTT1 null genotype may increase the risk of lung cancer among the East Asian populations © 2015, Spandidos Publications. All Rights Reserved.


Wang Y.,Henan Academy of Medical science | Dai Y.,Zhengzhou University | Li X.,Zhengzhou University | Chen C.,Zhengzhou University | And 2 more authors.
Acta Biologica Hungarica | Year: 2011

The effect of all-trans retinoic acid (atRA) on palatal fusion and the underlying mechanisms were investigated using organ culture. Compared with control group, the atRA-treated group (1 μM and 5 μM) had more medial edge epithelium (ME) remaining within the midline epithelial seam (MES). At 10 μM atRA, the opposing shelves were not in contact at the culture end (72 h). Cell death detection by TUNEL and laminin immunohistochemistry demonstrated that atRA (5 μM) induced apoptosis in mesenchyme and inhibited degradation of basal lamina within MES. Notably, migration and apoptosis of ME cells and degradation of basal lamina within MES markedly represented vehicle control palatal shelves in culture. Additionally, apoptosis was not detected in mesenchyme of control palatal shelves. Immunoblotting analysis revealed that Smad2 and Smad3 were endogenously activated and expression of Smad7 was inhibited during the fusion process. In contrast, atRA treatment abrogated phosphorylation of Smad2 and Smad3 and inducible expression of Smad7 in ME. From these data, it is assumed that inhibition of Smad pathway by atRA in ME may play a critical role in abrogation of the ME cell apoptosis and degradation of the basal laminin, which might contribute to failure of palatal fusion. © 2011 Akadé miai Kiadó, Budapest.


Ma S.,Zhengzhou University | Liang S.,Zhengzhou University | Liang S.,Henan University of Science and Technology | Jiao H.,Zhengzhou University | And 6 more authors.
Molecular and Cellular Biochemistry | Year: 2014

Mesenchymal stem cells (MSCs) represent a potential therapeutic target for glioma. We determined the molecular mechanism of inhibitory effect of human umbilical cord-derived MSCs (hUC-MSCs) on the growth of C6 glioma cells. We demonstrated that hUC-MSCs inhibited C6 cell growth and modulated the cell cycle to G0/G1 phase. The expression of β-catenin and c-Myc was downregulated in C6 cells by conditioned media from hUC-MSCs, and the levels of secreted DKK1 were positively correlated with concentrations of hUCMSCs-CM. The inhibitory effect of hUC-MSCs on C6 cell proliferation was enhanced as the concentration of DKK1 in hUCMSCs-CM increased. When DKK1 was neutralized by anti-DKK1 antibody, the inhibitory effect of hUC-MSCs on C6 cells was attenuated. Furthermore, we found that conditioned media from hUC-MSCs transfection with siRNA targeting DKK1 mRNA or pEGFPN1-DKK1 plasmid lost or enhanced the abilities to regulate the Wnt signaling in C6 cells. Therefore, hUC-MSCs inhibited C6 glioma cell growth via secreting DKK1, an inhibitor of Wnt pathway, may represent a novel therapeutic strategy for malignant glioma. © 2013 Springer Science+Business Media New York.


Ma N.,Henan Academy of Medical science | Nie W.,Henan Academy of Medical science | Wang T.,Henan Academy of Medical science | Li C.,Henan Academy of Medical science
Life Science Journal | Year: 2013

Through the description of the epidemic characteristics of rare disease, we have realized the properties of the rare diseases and disease spectrum. In the US and EU, legislation including the Orphan Drug Act (1983) and the Orphan Regulation No 141/2000 has brought many rare disease treatments into clinical practice. Many problems in China on rarity including: less society's attention; difficulties in obtaining timely, accurate diagnoses; lack of experienced healthcare providers; useful, reliable and timely information may be hard to find; research activities are less common; developing new medicines may not be economically feasible; treatments are sometimes very expensive. Emphasis is required to support appropriate research and development leading to better prevention, diagnosis and treatments of rare diseases. Conclusions: In this article, the primary tasks faced by China have been proposed: to call on the government to legislate as soon as possible; to establish information platform of rare diseases and orphan drugs for sharing the global rare diseases resources; to establish Rare Disease Outpatient Service(RDOPS) for improving the level of diagnosis and treatment; to carry out tertiary prevention of the rare diseases; to establish the rare diseases epidemiological surveillance system in our country.


PubMed | Tianjin Medical University, Henan Academy of Medical science and Zhengzhou University
Type: Journal Article | Journal: Oncology letters | Year: 2015

The


PubMed | Henan Academy of Medical science
Type: Journal Article | Journal: Acta biologica Hungarica | Year: 2011

The effect of all-trans retinoic acid (atRA) on palatal fusion and the underlying mechanisms were investigated using organ culture. Compared with control group, the atRA-treated group (1 M and 5 M) had more medial edge epithelium (ME) remaining within the midline epithelial seam (MES). At 10 M atRA, the opposing shelves were not in contact at the culture end (72 h). Cell death detection by TUNEL and laminin immunohistochemistry demonstrated that atRA (5 M) induced apoptosis in mesenchyme and inhibited degradation of basal lamina within MES. Notably, migration and apoptosis of ME cells and degradation of basal lamina within MES markedly represented vehicle control palatal shelves in culture. Additionally, apoptosis was not detected in mesenchyme of control palatal shelves. Immunoblotting analysis revealed that Smad2 and Smad3 were endogenously activated and expression of Smad7 was inhibited during the fusion process. In contrast, atRA treatment abrogated phosphorylation of Smad2 and Smad3 and inducible expression of Smad7 in ME. From these data, it is assumed that inhibition of Smad pathway by atRA in ME may play a critical role in abrogation of the ME cell apoptosis and degradation of the basal laminin, which might contribute to failure of palatal fusion.

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