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Orlova N.A.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Kovnir S.V.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Vorobiev I.I.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Gabibov A.G.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Vorobiev A.I.,Research Center for Hematology
Acta Naturae | Year: 2013

Recombinant blood clotting factor VIII is one of the most complex proteins for industrial manufacturing due to the low efficiency of its gene transcription, massive intracellular loss of its proprotein during post-translational processing, and the instability of the secreted protein. Improvement in hemophilia A therapy requires a steady increase in the production of factor VIII drugs despite tightening standards of product quality and viral safety. More efficient systems for heterologous expression of factor VIII can be created on the basis of the discovered properties of its gene transcription, post-translational processing, and behavior in the bloodstream. The present review describes the deletion variants of factor VIII protein with increased secretion efficiency and the prospects for the pharmaceutical development of longer acting variants and derivatives of factor VIII. © 2013 Park-media, Ltd.


Iserkapov A.V.,Lomonosov Moscow University of Fine Chemical Technology | Berkovskiy A.L.,Research Center for Hematology | Gurvits I.D.,Research Center for Hematology | Shastina N.S.,Lomonosov Moscow University of Fine Chemical Technology | Shvets V.I.,Lomonosov Moscow University of Fine Chemical Technology
Russian Journal of Biopharmaceuticals | Year: 2014

In this article the study of method for producing of activated prothrombin complex having factor VIII- bypassing activity has been presented. The method consists in the isolation of prothrombin complex with a high content of Factor VII by anion exchange chromatography from plasma at volume on DEAE-Sephadex A50 and its subsequent activation by the action of low concentrations of calcium ions. Also the influence of different conditions (calcium ion concentration, pH, temperature, time) on the efficiency of the activation process has been studied. The optimal conditions are calcium concentration 0,85 mM, pH 7,60, temperature 15°C during the process within 24 hours in the studied range. © 2014 Folium Ltd. All rights reserved.

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