Hemato onkologicka Klinika LF UP

Olomouc, Czech Republic

Hemato onkologicka Klinika LF UP

Olomouc, Czech Republic
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Szotkowski T.,Hemato onkologicka klinika LF UP | Cernan M.,Hemato onkologicka klinika LF UP | Hubacek J.,Hemato onkologicka klinika LF UP | Holzerova M.,Hemato onkologicka klinika LF UP | Papajik T.,Hemato onkologicka klinika LF UP
Onkologie (Czech Republic) | Year: 2017

Advances achieved in anticancer treatment are partly redeemed by adverse events. So called therapy-related acute myeloid leukemia belongs to the most serious ones. Their incidence growth is one of the most rapid among neoplastic disorders nowadays. Causes of developement of these complications are not clearly clarified. Several pathogenetic mechanisms involvement is anticipated. Generally speaking, prognosis of these disorders is very poor, partly due to toxicity of previous antitumour treatment, partly due to adverse biological character of this leukemia group. The therapeutic approach depends on patient`s performance status as well as genetic prognosis of the disease itself. Allogeneic hematopoietic cells transplantation is the only potentially curative option for majority of patients. Reduction of incidence of the disease and expansion of therapeutic possibilities are very important tasks of both oncology and heamatology.


Pika T.,Hemato onkologicka Klinika LF UP | Lochman P.,Oddeleni Klinicke Biochemie | Latalova P.,Ustav Klinicke A Molekularni Patologie | Minarik J.,Hemato onkologicka Klinika LF UP | And 3 more authors.
Klinicka Biochemie a Metabolismus | Year: 2015

Introduction: AL amyloidosis is a rare disease belonging to the group of monoclonal gammopathies. The basis of the disease is the deposition of fibrils formed by molecules of monoclonal immunoglobulin light chains into the form of amyloid - amorphous proteinaceous material. Detection and quantification of monoclonal immunoglobulin (MIg) or immunoglobulin light chains is a key aspect in the diagnosis and monitoring of patients with AL amyloidosis. Aim: The content of this paper is our own experience with the determination of MIg in patients with AL amyloidosis. Patients and methods: The analyzed group included 27 patients with systemic AL amyloidosis. In all patients we performed the detection and quantification of monoclonal immunoglobulins in serum and urine using electrophoresis and immunofixation, serum free light chain levels were determined by FreeLite™ system. In 13 patients we performed determination of heavy / light chain pairs (HLC) of immunoglobulins, using HevyLite™ system. Results: Immunofixation and electrophoresis of samples showed the presence of molecules MIg in 17/27 (63%) patients and the median concentration was 3.8 g/l (0.3 to 14.2 g/l). By immunofixation there were 5 cases of complete molecules of the IgG isotype (1x IgG-κ, 4x IgG-λ), in 4 cases IgA-λ and in one case IgD-λ. In 7 patients we detected only λ light chains in serum. When analyzing the urine excretion, Bence - Jones protein was detected in 15 (56%) patients. In all cases the quantity was over 200 mg/24 hours, while in 3 patients we found the presence of complete molecules of MIg in urine (IgA λ 1x, 2x IgG-λ). Analysis of serum free light chains showed abnormal values in all patients with median of 310 mg/l (53.6 - 1562 mg/l), pathology of κ/λ ratio was found in 25 patients (92.5%). Analyses of the HLC levels were performed in 13 patients. In the case of IgA isotype we detected pathology of IgA-λ HLC levels along with the change in HLC ratio in 3/4 patients. In IgG isotype, pathology of IgG-λ levels and HLC ratio was detected in 2/2 patients. In the case of λ and immunofixation negative sera there was no elevation of HLC levels in any of the isotypes. Conversely, in 5/7 patients we observed suppression of IgG-κ HLC combined with the change of HLC ratio. IgA and IgM isotype pair suppression was not observed. Conclusion: Detection, quantification and complex analysis of monoclonal immunoglobulin belongs to the key aspects of care for patients with AL amyloidosis. The combination of standard techniques of electrophoresis and immunofixation together with determination of serum free light chains allows monitoring of majority of the patients with AL amyloidosis. To clarify the contribution of HLC test, analysis of more extensive group of patients is needed.

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