Hellenic Agriculture Organization Veterinary Research Institute of Thessaloniki

Thérmi, Greece

Hellenic Agriculture Organization Veterinary Research Institute of Thessaloniki

Thérmi, Greece

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Mastranestasis I.,University of Aegean | Mastranestasis I.,Hellenic Agriculture Organization Veterinary Research Institute of Thessaloniki | Kominakis A.,National and Kapodistrian University of Athens | Hager-Theodorides A.L.,National and Kapodistrian University of Athens | And 3 more authors.
Small Ruminant Research | Year: 2016

The aim of the present study was to investigate the potential associations between polymorphisms of microsatellite markers and various traits in the Lesvos dairy sheep. In total, 350 individuals using 11 microsatellite markers were genotyped and various traits including six body anatomical measurements as well as milk yield and litter size were considered. The adaptive Least Absolute Shrinkage and Selection Operator method was employed to detect significant associations between markers’ alleles and the various phenotypic traits. In all but one microsatellite loci, rare alleles were detected. Markers OARJMP29, OARFCB128 and OARCP38 were found to be associated with milk yield. Marker OARJMP29 was also associated with body weight, girth circumference and horn length. Marker OARFCB128 was also significant for tail length, tail width and girth circumference while marker OARCP38 was significant for body length, as well. Current results shed more light in the associations between marker polymorphisms with various phenotypic traits in dairy sheep, including traits of economic importance that could potentially be implemented in a marker assisted selection program for increasing production efficiency in the Lesvos breed. © 2016 Elsevier B.V.


Mastranestasis I.,University of Aegean | Mastranestasis I.,Hellenic Agriculture Organization Veterinary Research Institute of Thessaloniki | Ekateriniadou L.V.,Hellenic Agriculture Organization Veterinary Research Institute of Thessaloniki | Ligda C.,Hellenic Agriculture Organization Veterinary Research Institute of Thessaloniki | Theodorou K.,University of Aegean
Small Ruminant Research | Year: 2015

We investigated, through the use of 11 microsatellite molecular markers, the genetic diversity and structure of 350 individuals from ten flocks of the Lesvos sheep breed population (Greece). The results revealed that although the breed shows particularly high levels of observed heterozygosity (H o =0.83), the mean number of alleles of 6.8 is somewhat lower than in other sheep breeds. F IS (-0.143±0.065) was significantly negative over loci for the five out of the ten flocks and the observed heterozygosity was consistently higher than the expected for all flocks. The low value of F ST (0.021±0.003) implies that the quasi-totality of genetic variability is due to within-flock variability, suggesting a strong genetic cohesion of the total population. Furthermore, we revealed a breeding practice that comprises an important exchange of animals: the number of migrants between flocks ranged from 6.41 to 53.92. These exchanges happen both between neighboring and more distant farms as indicated by the correspondence analysis and the neighbor-joining dendrogram. This is also reflected in the clustering obtained by STRUCTURE analysis according to which all flocks had comparable proportions of membership to the five clusters detected. In conclusion, the implemented management practice confers high gene variability both at the flock level and in the total population which is very promising for the conservation of the breed as a genetic resource. However, in conjunction with the isolation of the breed on the island of Lesvos, the high gene flow among flocks may also be responsible for the rather low mean number of alleles as it decreases the overall effective size. Moreover, it is still unknown whether the present breeding practice meets adequately production objectives of the breeders, which is the other important facet of the breed management. © 2015 Elsevier B.V.

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