Heilongjiang Vocational Institute of Ecological Engineering

Harbin, China

Heilongjiang Vocational Institute of Ecological Engineering

Harbin, China
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Wang Y.,Heilongjiang Vocational Institute of Ecological Engineering | Shao Y.,Heilongjiang Vocational Institute of Ecological Engineering | Zou X.,Heilongjiang Vocational Institute of Ecological Engineering | Yang M.,Heilongjiang Vocational Institute of Ecological Engineering | Guo L.,Heilongjiang Vocational Institute of Ecological Engineering
Bioengineered | Year: 2017

With a set of perfect extracellular lignocellulolytic enzymes, white-rot fungus has been recognized as playing an important role in the degradation of lignocellulose materials, which leads to the possibility of creating a composite enzymatic system with high hydrolysis efficiency in vitro. Echinodontium taxodii is a promising white-rot fungus for biologic pretreatment. In this study, we extracted the extracellular products of E. taxodii under solid-state fermentation conditions, mixed the extracellular products with cellulase to build a composite enzymatic system, and systematically evaluated the effect of this system on the hydrolysis of acid-pretreated and raw maize stovers. We found that the extracellular products from E. taxodii could significantly improve the hydrolysis efficiency of cellulase, with a synergistic action between the extracellular products and cellulase. Corn stovers treated with extracellular products were suitable for the enzymatic hydrolysis of cellulase. Furthermore, we found that pure proteins from the extracellular products were not sufficient to generate synergistic action. This finding suggests that non-protein substances may also be involved in the synergistic action between the extracellular products and cellulase. © 2017 Taylor & Francis


Zhang J.-J.,Harbin University of Commerce | Li W.-L.,Harbin University of Commerce | Ding Z.-D.,Heilongjiang Vocational Institute of Ecological Engineering | Zang B.-S.,Harbin University of Commerce | Zhu F.-F.,Harbin University of Commerce
Chinese Journal of New Drugs | Year: 2013

Metabonomics, a newly developed branch of genomics, has been becoming an important component in researching the material foundation for the effectiveness of traditional Chinese medicine. Metabonomics is one of the important platforms in this research field. It can be directly applied to wholly understand the physiological and biochemical states by its "metabolome profile". Especially, it is of important theoretical significance and application value in studies of toxicology, diagnosis, traditional Chinese medicine and plants including natural medicines. Metabonomics will extend research ideas on material basis of the traditional Chinese medicine compounds.


An F.-X.,Northeast Agricultural University | An F.-X.,Heilongjiang Academy of science | Lu B.-W.,Heilongjiang Vocational Institute of Ecological Engineering | Liang M.,Heilongjiang Academy of science | And 2 more authors.
Zhiwu Shengli Xuebao/Plant Physiology Journal | Year: 2014

MicroRNAs (miRNAs), as endogenous small non-coding single-stranded RNAs of 16-29 nt, play a prominent role in the process of growth, development and responses to environmental stresses in plants. The miRNAs in response to low temperature in Hemerocallis fulva roots were identified using deep-sequencing technique in combination with bioinformatics prediction. A total of 14 843 184 and 16 072 575 RNA sequences were explored under normal and low temperature conditions, which represented 14 064 385 and 15 309 725 types of small RNA (sRNA), respectively. The sRNA showed a normal distribution. Through GenBank and Rfam comparison analysis, rRNA and tRNA accounts for a larger proportion in non-coding RNA. Totally 799 994 sRNA in 67 411 types were annotated under low temperature, and 1 055 466 sRNAs in 66 524 types were annotated under normal temperature. miR393, miR397 and miR396 were up-regulated and miR319 was downregulated at low temperature. This research provides rich data for illuminating the regulatory mechanism of protein synthesis and screening the key regulatory genes in response to low temperature.


Huang S.,Northeast Forestry University | Huang S.,Heilongjiang Vocational Institute of Ecological Engineering | Sun X.,Northeast Forestry University | Zhang Y.,Northeast Forestry University | And 2 more authors.
Journal of Forestry Research | Year: 2016

Nutrient retranslocation in trees is important in nutrient budgets and energy flows in forest ecosystems. We investigated nutrient retranslocation in the fine roots of a Manchurian Ash (Fraxinus mandshurica) and a Larch (Larix olgensis) plantation in northeastern China. Nutrient retranslocation in the fine roots was investigated using three methods, specifically, nutrient concentration, the ratio of Ca to other elements (Ca/other elements ratio) and nutrient content. The method based on nutrient content proved most suitable when investigating nutrient retranslocation from fine roots of the two species. The nutrient-content-based method showed that there were retranslocations of N, P, K and Mg from the fine roots of Manchurian Ash, with retranslocation efficiencies of 13, 25, 65, and 38 %, respectively, whereas there were no Ca retranslocations. There were retranslocations of N, P, K, Ca and Mg from the fine roots of Larch, with retranslocation efficiencies of 31, 40, 52, 23 and 25 %, respectively. © 2016 Northeast Forestry University and Springer-Verlag Berlin Heidelberg


Lu Q.,Northeast Forestry University | Zhu M.,Heilongjiang Vocational Institute of Ecological Engineering | Zu Y.,Northeast Forestry University | Liu W.,Northeast Forestry University | And 5 more authors.
Food Chemistry | Year: 2012

Nowadays, lignin antioxidant study is a hot spot. But some properties of lignin make it hard for a wide range of application. To improve lignin antioxidant activity, we employed supercritical antisolvent (SAS) method for preparation of nanoscale lignin (0.144 ± 0.03 μm) using acetone as a solvent and supercritical carbon dioxide as an antisolvent. The nanoscale lignin was characterised by Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR) and X-ray Diffraction (XRD). The results showed that SAS process did not result in lignin degradation or chemical structure change. Due to solubility increase, many antioxidant parameters of the nanoscale lignin were obviously enhanced, including DPPH radical scavenging activity, superoxide radical scavenging activity and reducing power. As an antioxidant, the nanoscale lignin was a better material than the non-nanoscale lignin. Our study was to promote the lignin application in animal husbandry, pharmaceutical and food processing industries. © 2012 Elsevier Ltd. All rights reserved.


Zhu M.,Northeast Forestry University | Fang G.,Northeast Forestry University | Han S.,Northeast Forestry University | Rong H.,Heilongjiang Vocational Institute of Ecological Engineering | And 3 more authors.
Advanced Materials Research | Year: 2011

Lignin was extracted from Acanthopanax senticosus remainders after pharmaceutical production process, with acetic acid-water as extracting reagent. Four factors effects, namely reaction temperature from 160°C to 220°C, reaction time from 40 min to 100 min, acetic acid concentration from 50% to 80% and solid-liquid ratio from 1:40 to 1:5, were studied and optimized by a four-level orthogonal array design (OAD) on lignin antioxidant capacity. The results indicated the reaction temperature and reaction time were significant factors among these factors. At the same lignin solution concentration, the acetic acid lignin antioxidant capacity was better than that of Kraft lignin, but much lower than that of butylated hydroxyanisole (BHA). © (2011) Trans Tech Publications, Switzerland.


Hu Y.,Northeast Forestry University | Wang S.,Northeast Forestry University | Li R.,Heilongjiang Vocational Institute of Ecological Engineering
Taiyangneng Xuebao/Acta Energiae Solaris Sinica | Year: 2011

In order to find the reasonable logistics distribution timing and optimun material processing method, this paper starts researching various desert shrub's water ratios by kinds of processing methods on the basis of a lot of measurements, analytical and statistical work and aims at giving a reference and a feasible method to biomass generation plants, which pursue the goal of reducing generation cost and improving their market competitiveness.


Zhu M.-H.,Northeast Forestry University | Zhu M.-H.,Heilongjiang Vocational Institute of Ecological Engineering | Fang G.-Z.,Northeast Forestry University | Han S.-Y.,Northeast Forestry University | And 4 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2012

Lignin was extracted from Acanthopanax senticosus remainders coming from pharmaceutical production process with acetone as extracting reagent. Lignin antioxidant capacity and yield were tested under single-factor experiment, such as reaction temperature, reaction time, solid-liquid ratio, acetic acid concentration, and solution concentration of acetone. The optimal conditions by a four-level orthogonal array design were as followings: reaction temperature 180°C, solution concentration of acetone 80%, solid-liquid ratio 1:20, and reaction time 80 min. The EC 50 of the acetone organic lignin on DPPH was 1.518 mg/mL by verification experiments. The results indicate that the acetone organic lignin antioxidant capacity is better than that of Kraft lignin, but much lower than that of butylated hydroxyanisole (BHA).


Dai M.,Shenzhen University | Wang P.,University of Sydney | Zhang S.,Shenzhen University | Chen B.,Shenzhen University | And 3 more authors.
Transactions on Emerging Telecommunications Technologies | Year: 2014

Cooperative communication has the potential of providing better throughput and reliability to wireless systems when compared with direct communication. To realise the potential gain, it is important to design cooperative strategies for some representative scenarios. This survey deals with three basic wireless relay channels, namely, the parallel relay channel, the multiple-access relay channel and the broadcast relay channel. For the first channel, which models a single unicast connection, various forwarding strategies are studied. For the second and third channels, which model, respectively, the uplink and downlink scenarios with multiple unicast connections; network codes that exploit the possibility of coding among the connections are studied. The common aim pertaining to the studies of all these three channels is to use the limited radio resource in the most efficient way. Beside the aforementioned conventional works, studies that have state-of-the-art assumptions for relay networks, including outdated channel state information at the transmitter, full duplex relay and practical rateless-coded cooperation, are also extensively reviewed. Copyright © 2014 John Wiley & Sons, Ltd.

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